Human CD114/CSF3R/GCSFR Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description

CD114, also known as CSF3R and GCSFR (Granulocyte Colony Stimulating Factor Receptor), is a cell surface receptor for CSF3 (Colony Stimulating Factor 3) that plays a critical role in the proliferation, differentiation, and activation of hematopoietic cells of the neutrophil lineage. CD114 is a member of the cytokine receptor superfamily and the immunoglobulin (Ig) superfamily that may also function in some other cell surface adhesion or recognition processes. CD114 contains an immunoglobulin (Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region (ECD). CD114 is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of its ligand induces dimerization or oligomerization of this receptor and leads to the activation of several intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase (Lyn and Syk) pathways. CD114 thus plays important functional roles in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G­CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood.

  

 

Gene Symbol: CD114; GCSFR; CSF3R

 

NCBI Gene ID: 1441

 

Uniprot Entry: Q99062

 

Construct Details: The recombinant human CD114-Fc fusion is expressed as a 839 amino acid protein consisting of Glu25 - Glu625 region of CD114 (Uniprot Accession #Q99062) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD114-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

ECGHISVSAP IVHLGDPITA SCIIKQNCSH LDPEPQILWR LGAELQPGGR QQRLSDGTQE  

        70         80         90        100        110        120 

SIITLPHLNH TQAFLSCCLN WGNSLQILDQ VELRAGYPPA IPHNLSCLMN LTTSSLICQW  

       130        140        150        160        170        180 

EPGPETHLPT SFTLKSFKSR GNCQTQGDSI LDCVPKDGQS HCCIPRKHLL LYQNMGIWVQ 

       190        200        210        220        230        240 

AENALGTSMS PQLCLDPMDV VKLEPPMLRT MDPSPEAAPP QAGCLQLCWE PWQPGLHINQ 

       250        260        270        280        290        300 

KCELRHKPQR GEASWALVGP LPLEALQYEL CGLLPATAYT LQIRCIRWPL PGHWSDWSPS 

       310        320        330        340        350        360 

LELRTTERAP TVRLDTWWRQ RQLDPRTVQL FWKPVPLEED SGRIQGYVVS WRPSGQAGAI 

       370        380        390        400        410        420 

LPLCNTTELS CTFHLPSEAQ EVALVAYNSA GTSRPTPVVF SESRGPALTR LHAMARDPHS 

       430        440        450        460        470        480 

LWVGWEPPNP WPQGYVIEWG LGPPSASNSN KTWRMEQNGR ATGFLLKENI RPFQLYEIIV  

       490        500        510        520        530        540 

TPLYQDTMGP SQHVYAYSQE MAPSHAPELH LKHIGKTWAQ LEWVPEPPEL GKSPLTHYTI 

       550        560        570        580        590        600 

FWTNAQNQSF SAILNASSRG FVLHGLEPAS LYHIHLMAAS QAGATNSTVL TLMTLTPEGS 

       610        620        630        640        650        660 

ESTTENLYFQ GSTGTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH 

       670        680        690        700        710        720 

EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 

       730        740        750        760        770        780 

PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE 

       790        800        810        820        830 

NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 93.7; Estimated by SDS-PAGE under reducing condition (kDa): 100-110

 

Calculated PI: 6.22

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 178770

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds CSF3/G-CSF and inhibits CSF3/G­CSF­induced proliferation of NFS-60 myelogenous leukemia lymphoblast cells with a typical ED₅₀ of 0.5-3 μg/ml

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. J. Exp. Med. 172:1559 (1989) 

2. EMBO J. 10:2855 (1990) 

3. Proc. Natl. Acad. Sci. 91:4480 (1993)

4. J. Exp. Med. 190:497 (1998)

5. Front. Biosci. 12:800 (2006)

 

 

 

 

Product datasheet (pdf) can be downloaded here: FCL2197-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.