Human CD73/NT5E/NTE/5’-NT Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description

CD73, also known as 5’-NT, NT5 and NTE, is a glycosyl-phosphatidyl-inositol (GPI) anchored cell membrane protein that belongs to the 5'-nucleotidase (5’-NT) family. The native enzyme exists as a homodimer on the cell surface. CD73 is a purine salvage enzyme expressed on human T and B lymphocytes. CD73 converts extracellular nucleoside­5'­monophosphates to membrane permeable nucleosides, with AMP as the preferred substrate. It also exhibits AMP-, NAD-, and NMN-nucleosidase activities.  CD73 is one of several ectoenzymes responsible for the production of extracellular adenosine, a signaling molecule involved in many physiologic processes, including inflammation, tissue injury response, and cholinergic synapses. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors (GPCR) to induce intracellular responses. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'­nucleotidase activity. CD73 mediates lymphocyte-stromal cell interactions to facilitate lymphocyte development/function.  CD73 has also been considered as a lymphocyte maturation marker independent of its catalytic activity. CD73 serves as a co-stimulatory molecule in activating T cells. The mutation in CD73 gene is associated with calcification of joints and arteries (CALJA), a condition characterized by adult-onset calcification of the lower extremity arteries  and hand and foot capsule joints.

 

 

Gene Symbol: CD73; 5’-NT; NT; eN; NT5; NTE; eNT; E5NT; CALJA

 

NCBI Gene ID: 4907

 

Uniprot Entry: P21589

 

Construct Details: The recombinant human CD73-Fc fusion is expressed as a 759 amino acid protein consisting of Trp27 - Lys547 region of CD73 (Uniprot Accession #P21589) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing CD73-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ 

        70         80         90        100        110        120 

YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA 

       130        140        150        160        170        180 

KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK 

       190        200        210        220        230        240 

LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI 

       250        260        270        280        290        300 

VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK 

       310        320        330        340        350        360 

WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHA DETFWNHVSM 

       370        380        390        400        410        420 

CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST 

       430        440        450        460        470        480 

GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD 

       490        500        510        520        530        540 

GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI KSTTENLYFQ GSTGTHTCPP 

       550        560        570        580        590        600 

CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK 

       610        620        630        640        650        660 

TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV 

       670        680        690        700        710        720 

YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS 

       730        740        750 

KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 84.4; Estimated by SDS-PAGE under reducing condition (kDa): 85-90

 

Calculated PI: 6.41 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 93585

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: CD73 hydrolyzes the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP) with a specific activity over 20,000 pmoles/min/μg.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1.  Cell Signal. 9: 131 (1997)

2. Immunol. Rev. 161:95 (1998).

3. Eur J Immunol. 28: 2981 (1998)

4. J. Biol. Chem. 278:13468 (2003)

5. J Immunol. 180: 4246 (2008)

 

 

 

 

Product datasheet (pdf) can be downloaded here: FCL2199-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.