Human Oct4, Sox2, Klf4, c-Myc Genes in Lentivector, Endotoxin-free DNA

Description

Terminally differentiated somatic cells can be reprogrammed to generate induced pluripotent stem (iPS) cells by overexpression of a defined set of transcription factors. Typically OCT4, SOX2, c-Myc and KLF4 (OKSM) are the 4 reprogramming factors that together are sufficient to reprogram differentiated cells to an embryonic-like state termed iPS (induced pluripotent stem) cells. The OKSM combination was identified initially by screening 24 pre-selected factors in mouse embryonic fibroblasts (MEFs). The iPS cells are morphologically and phenotypically similar to embryonic stem (ES) cells and offer exciting possibilities in stem cell research and regenerative medicine. This lentivector gene clone contains all these 4 human reprogramming transcription factors that are expressed from a single transcript using 2A peptide-mediated self-cleaving elements: Oct4-2A-Sox2-2A-Klf4-2A-c-Myc. 

 

 

Gene Symbol: Oct-4; Sox2;Klf4; c-Myc

 

NCBI Gene ID: 5460; 6657; 9314; 4609

 

Uniprot Entry: Q01860; P48431; O43474; P01106

 

Construct Details: Full length human Oct4, Sox2, Klf4 and c-Myc genes linked by self-cleaving 2A-elements is subcloned into the Lentiviral expression vector pLTE with an an upstream EF1α promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. 

 

Vector Type: pLTE or pLTE-IRES-Marker (lentiviral expression vector containing a heterologous  EF1α promoter which is active in both human and murine cells, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 5070 (bp)

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Cell. 126:663 (2006)

2. Cell 131:861 (2007)

3. Science. 318:1917 (2007)

4. Cell 136:411 (2009)

5. Stem Cells 27:543 (2009)

 

 

 

 

Product datasheet (pdf) can be downloaded here: LTE1099-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.