Human EDA2R Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
EDA2R (ectodysplasin A2 receptor), also known as XEDAR (Xlinked Ectodysplasin Receptor), is a single-pass, type III transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF27). The extracellular domain (ECD) of EDA2R contains three TNFR cysteinerich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. The cytoplasmic region of EDA2R lacks a death domain (DD) that is found in EDAR and other TNFRSF members. EDA2R is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Other than a 2 aa deletion in its TNFlike domain, EDAA2 is identical to EDAA1. Mutations in EDA give rise to a clinical syndrome hypohidrotic ectodermal dysplasia (HED), which is characterized by loss of hair, sweat glands, and teeth. EDA2R specifically binds to EDA-A2 isoform but not EDA-A1. EDA2R itself is strongly associated with androgenetic alopecia (male hair loss). Even though it does not contain a cytoplasmic death domain, EDA2R can associate with Fas and induce p53-dependent cell death. EDA2R is down-regulated in breast, colon, and lung cancers, particularly in patients with p53 mutations. EDA2R also plays a role in EDA-A2 induced skeletal muscle degeneration and osteoblast differentiation.
Gene Symbol: XEDAR; EDAA2R; EDA-A2R; TNFRSF27; UNQ2448/PRO5727/PRO34080
NCBI Gene ID: 60401
Uniprot Entry: Q9HAV5
Construct Details: The recombinant human EDA2R-Fc fusion is expressed as a 366 amino acid protein consisting of Asp2 - Thr138 region of EDA2R (UniProt accession #Q9HAV5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source: Human cells stably expressing human EDA2R-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
MDCQENEYWD QWGRCVTCQR CGPGQELSKD CGYGEGGDAY CTACPPRRYK SSWGHHRCQS
70 80 90 100 110 120
CITCAVINRV QKVNCTATSN AVCGDCLPRF YRKTRIGGLQ DQECIPCTKQ TPTSEVQCAF
130 140 150 160 170 180
QLSLVEADTP TVPPQEATST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC
190 200 210 220 230 240
VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC
250 260 270 280 290 300
KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW
310 320 330 340 350 360
ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL
SLSPGK
M.W.: Calculated molecular mass (kDa): 40.9; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI: 6.74
Calculated extinction coefficients (M-1 cm-1, at 280nm): 60735
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")
Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity: Binds to its ligand EDA-A2 and blocks EDA-A2-mediated signaling activity.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
1. Science 290:523 (2000).
2. J. Biol. Chem. 277:44953 (2002).
3. Mol. Cell. Biol. 24:1608 (2004).
4. Oncogene 28:3081 (2009).
5. Clin. Cancer Res. 16:1140 (2010).
Product datasheet (pdf) can be downloaded here: FCL0301-PDS.pdf
Additional supporting documents, including COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Human EDA2R Full-length Gene in Lentivector, Endotoxin-free DNA
Human EDAR ECD Protein, (Extracellular Domain), Fc-fusion, Recombinant