Human FCRL1/FcRH1/CD307a/IFGP1/IRTA5 Protein, ECD (Extracellular Domain), Recombinant

FCRL1 (Fc receptor-like protein 1), also known as FcRH1 (Fc receptor homolog 1) and CD307a, is a single-pass type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (IgSF).  FCRL1 consists of an extracellular domain (ECD) with 3 C2-like immunoglobulin motifs, a transmembrane domain and a cytoplasmic domain with 5 immunoreceptor tyrosine-based inhibitory motifs (ITIM). FCRL1 represents one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. FCRL1 is preferentially expressed by B-cells that regulates BCR-mediated signaling responses and recruits ABL1 as the intracellular effector to enhance B-cell activation. FCRL1 may also play a role in the regulation of cancer cell growth.

Gene Symbol: FCRL1; FCRH1; IFGP1; hIFGP1; IRTA5; CD307a

NCBI Gene ID: 115350

Uniprot Entry: Q96LA6

Construct Details: The recombinant human FCRL1 ECD protein is expressed as a 313 amino acid protein consisting of Ala17 - Gly308 region of FCRL1 and a C-terminal His-tag.

Source: Human cells stably expressing FCRL1 ECD protein and growing in chemical-defined media with no animal component or antibiotics

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
AELFLIASPS HPTEGSPVTL TCKMPFLQSS DAQFQFCFFR DTRALGPGWS SSPKLQIAAM

        70         80         90        100        110        120
WKEDTGSYWC EAQTMASKVL RSRRSQINVH RVPVADVSLE TQPPGGQVME GDRLVLICSV

       130        140        150        160        170        180
AMGTGDITFL WYKGAVGLNL QSKTQRSLTA EYEIPSVRES DAEQYYCVAE NGYGPSPSGL

       190        200        210        220        230        240
VSITVRIPVS RPILMLRAPR AQAAVEDVLE LHCEALRGSP PILYWFYHED ITLGSRSAPS

       250        260        270        280        290        300
GGGASFNLSL TEEHSGNYSC EANNGLGAQR SEAVTLNFTV PTGARSNHLT SGSTGYPYDV

       310
PDYAGHHHHH HHH

M.W.: Calculated molecular mass 34.2 kDa; estimated by SDS-PAGE under reducing condition 45-50 kDa probably due to glycosylation

Calculated PI: 6.05

Calculated extinction coefficients (M-1 cm-1, at 280nm): 45755

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.