Human APLP1/APLP Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant

Description

APLP1 (Amyloid beta precursor like protein 1) is a single-pass type I transmembrane protein of the highly conserved APP (amyloid precursor protein) family. APP functions as a cell surface receptor and may play a role in neuron synaptic maturation during cortical development.  APLP1 binds zinc and copper in the extracellular domain (ECD) but with no Cu2+ reducing activity.  APLP1 can be cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates a C-terminal cytoplasmic fragment that may act as a transcriptional activator. APLP1 may interact with G-protein signaling pathways and regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.  The cleaved gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. 

 

 

Gene Symbol: APLP1;  APLP; C30

 

NCBI Gene ID: 333

 

Uniprot Entry: P51693

 

Construct Details: The recombinant human APLP1-Fc fusion protein is expressed as a 770 amino acid protein consisting of Ser39 - Glu580 region of APLP1 and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

   

Source: Human cells stably expressing APLP1-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

SLAGGSPGAA EAPGSAQVAG LCGRLTLHRD LRTGRWEPDP QRSRRCLRDP QRVLEYCRQM  

        70         80         90        100        110        120 

YPELQIARVE QATQAIPMER WCGGSRSGSC AHPHHQVVPF RCLPGEFVSE ALLVPEGCRF 

       130        140        150        160        170        180 

LHQERMDQCE SSTRRHQEAQ EACSSQGLIL HGSGMLLPCG SDRFRGVEYV CCPPPGTPDP 

       190        200        210        220        230        240 

SGTAVGDPST RSWPPGSRVE GAEDEEEEES FPQPVDDYFV EPPQAEEEEE TVPPPSSHTL 

       250        260        270        280        290        300 

AVVGKVTPTP RPTDGVDIYF GMPGEISEHE GFLRAKMDLE ERRMRQINEV MREWAMADNQ 

       310        320        330        340        350        360 

SKNLPKADRQ ALNEHFQSIL QTLEEQVSGE RQRLVETHAT RVIALINDQR RAALEGFLAA  

       370        380        390        400        410        420 

LQADPPQAER VLLALRRYLR AEQKEQRHTL RHYQHVAAVD PEKAQQMRFQ VHTHLQVIEE  

       430        440        450        460        470        480 

RVNQSLGLLD QNPHLAQELR PQIQELLHSE HLGPSELEAP APGGSSEDKG GLQPPDSKDD 

       490        500        510        520        530        540 

TPMTLPKGST EQDAASPEKE KMNPLEQYER KVNASVPRGF PFHSSEIQRD ELAPAGTGVS 

       550        560        570        580        590        600 

RESTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW 

       610        620        630        640        650        660 

YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS 

       670        680        690        700        710        720 

KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV 

       730        740        750        760        770 

LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

   

M.W.: Calculated molecular mass 86 kDa; estimated by SDS-PAGE under reducing condition: ~60-75 kDa major bands and ~90 kDa minor band  probably due to proteolytic cleavage

 

Calculated PI: 5.56

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  70455

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.