Human LSR/ILDR3/LISCH7/Angulin-1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

LSR (lipolysis stimulated lipoprotein receptor), also known as angulin-1, ILDR3 (immunoglobulin (Ig)-like domain containing receptor 3) and LISCH/LISCH7, belongs to the LISCH7 family of the Ig superfamily (IgSF).  LSR is a single-pass type I transmembrane proteins with an extracellular domain containing an Ig-like V-type motif, a transmembrane domain (TM), and a cytoplasmic tail enriched with basic/acidic/polar residues.  LSR is located in plasma membrane and tight junction.  LSR may be involved in establishment of skin barrier; protein localization to tricellular tight junction; and tricellular tight junction assembly. It is predicted to act upstream of or within maintenance of blood-brain barrier.  LSR maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions.  LSR also binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells. LSR may play a role in the clearance of triglyceride-rich lipoprotein from blood. Defects in LSR may be the cause of familial intrahepatic cholestasis.  

 

 

Gene Symbol: LSR; ILDR3; LISCH7; LISCH; Angulin-1

 

NCBI Gene ID: 51599

 

Uniprot Entry: Q86X29

 

Construct Details: Full length human LSR gene (encoding UniProt #Q86X29-1) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1950 (bp)

 

Gene Insert Sequence:  

ATGCAACAGGACGGACTTGGAGTAGGGACAAGGAACGGAAGTGGGAAGGGGAGGAGCGTGCACCCCTCCTGGCCTTGGTG

CGCGCCGCGCCCCCTAAGGTACTTTGGAAGGGACGCGCGGGCCAGACGCGCCCAGACGGCCGCGATGGCGCTGTTGGCCG

GCGGGCTCTCCAGAGGGCTGGGCTCCCACCCGGCCGCCGCAGGCCGGGACGCGGTCGTCTTCGTGTGGCTTCTGCTTAGC

ACCTGGTGCACAGCTCCTGCCAGGGCCATCCAGGTGACCGTGTCCAACCCCTACCACGTGGTGATCCTCTTCCAGCCTGT

GACCCTGCCCTGTACCTACCAGATGACCTCGACCCCCACGCAACCCATCGTCATCTGGAAGTACAAGTCTTTCTGCCGGG

ACCGCATCGCCGATGCCTTCTCCCCGGCCAGCGTCGACAACCAGCTCAATGCCCAGCTGGCAGCCGGGAACCCAGGCTAC

AACCCCTACGTTGAGTGCCAGGACAGCGTGCGCACCGTCAGGGTCGTGGCCACCAAGCAGGGCAACGCTGTGACCCTGGG

AGATTACTACCAGGGCCGGAGGATTACCATCACCGGAAATGCTGACCTGACCTTTGACCAGACGGCGTGGGGGGACAGTG

GTGTGTATTACTGCTCCGTGGTCTCAGCCCAGGACCTCCAGGGGAACAATGAGGCCTACGCAGAGCTCATCGTCCTTGGG

AGGACCTCAGGGGTGGCTGAGCTCTTACCTGGTTTTCAGGCGGGGCCCATAGAAGACTGGCTCTTCGTGGTTGTGGTATG

CCTGGCTGCCTTCCTCATCTTCCTCCTCCTGGGCATCTGCTGGTGCCAGTGCTGCCCGCACACTTGCTGCTGCTACGTCA

GGTGCCCCTGCTGCCCAGACAAGTGCTGCTGCCCCGAGGCCCTGTATGCCGCCGGCAAAGCAGCCACCTCAGGTGTTCCC

AGCATTTATGCCCCCAGCACCTATGCCCACCTGTCTCCCGCCAAGACCCCACCCCCACCAGCTATGATTCCCATGGGCCC

TGCCTACAACGGGTACCCTGGAGGATACCCTGGAGACGTTGACAGGAGTAGCTCAGCTGGTGGCCAAGGCTCCTATGTAC

CCCTGCTTCGGGACACGGACAGCAGTGTGGCCTCTGAAGTCCGCAGTGGCTACAGGATTCAGGCCAGCCAGCAGGACGAC

TCCATGCGGGTCCTGTACTACATGGAGAAGGAGCTGGCCAACTTCGACCCTTCTCGACCTGGCCCCCCCAGTGGCCGTGT

GGAGCGGGCCATGAGTGAAGTCACCTCCCTCCACGAGGACGACTGGCGATCTCGGCCTTCCCGGGGCCCTGCCCTCACCC

CGATCCGGGATGAGGAGTGGGGTGGCCACTCCCCCCGGAGTCCCAGGGGATGGGACCAGGAGCCCGCCAGGGAGCAGGCA

GGCGGGGGCTGGCGGGCCAGGCGGCCCCGGGCCCGCTCCGTGGACGCCCTGGACGACCTCACCCCGCCGAGCACCGCCGA

GTCAGGGAGCAGGTCTCCCACGAGTAATGGTGGGAGAAGCCGGGCCTACATGCCCCCGCGGAGCCGCAGCCGGGACGACC

TCTATGACCAAGACGACTCGAGGGACTTCCCACGCTCCCGGGACCCCCACTACGACGACTTCAGGTCTCGGGAGCGCCCT

CCTGCCGACCCCAGGTCCCACCACCACCGTACCCGGGACCCTCGGGACAACGGCTCCAGGTCCGGGGACCTCCCCTATGA

TGGGCGGCTACTGGAGGAGGCTGTGAGGAAGAAGGGGTCGGAGGAGAGGAGGAGACCCCACAAGGAGGAGGAGGAAGAGG

CCTACTACCCGCCCGCGCCGCCCCCGTACTCGGAGACCGACTCGCAGGCGTCCCGAGAGCGCAGGCTCAAGAAGAACTTG

GCCCTGAGTCGGGAAAGTTTAGTCGTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.