Human GPR61 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
GPR61 (G-protein coupled receptor 61) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family. GPR61 is most closely related to biogenic amine receptors. GPR61 is an orphan GPCR that constitutively activates the G(s)-alpha/cAMP signaling pathway. GPR61 shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B through receptor heteromerization. GPR61 may be involved in the regulation of food intake and body weight.
Gene Symbol: GPR61; BALGR; GPCR3
NCBI Gene ID: 83873
Uniprot Entry: Q9BZJ8
Construct Details: Full length human GPR61 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 1356 (bp)
Gene Insert Sequence:
ATGGAGTCCTCACCCATCCCCCAGTCATCAGGGAACTCTTCCACTTTGGGGAGGGTCCCTCAAACCCCAG
GTCCCTCTACTGCCAGTGGGGTCCCGGAGGTGGGGCTACGGGATGTTGCTTCGGAATCTGTGGCCCTCTT
CTTCATGCTCCTGCTGGACTTGACTGCTGTGGCTGGCAATGCCGCTGTGATGGCCGTGATCGCCAAGACG
CCTGCCCTCCGAAAATTTGTCTTCGTCTTCCACCTCTGCCTGGTGGACCTGCTGGCTGCCCTGACCCTCA
TGCCCCTGGCCATGCTCTCCAGCTCTGCCCTCTTTGACCACGCCCTCTTTGGGGAGGTGGCCTGCCGCCT
CTACTTGTTTCTGAGCGTGTGCTTTGTCAGCCTGGCCATCCTCTCGGTGTCAGCCATCAATGTGGAGCGC
TACTATTACGTAGTCCACCCCATGCGCTACGAGGTGCGCATGACGCTGGGGCTGGTGGCCTCTGTGCTGG
TGGGTGTGTGGGTGAAGGCCTTGGCCATGGCTTCTGTGCCAGTGTTGGGAAGGGTCTCCTGGGAGGAAGG
AGCTCCCAGTGTCCCCCCAGGCTGTTCACTCCAGTGGAGCCACAGTGCCTACTGCCAGCTTTTTGTGGTG
GTCTTTGCTGTCCTTTACTTTCTGTTGCCCCTGCTCCTCATACTTGTGGTCTACTGCAGCATGTTCCGAG
TGGCCCGCGTGGCTGCCATGCAGCACGGGCCGCTGCCCACGTGGATGGAGACACCCCGGCAACGCTCCGA
ATCTCTCAGCAGCCGCTCCACGATGGTCACCAGCTCGGGGGCCCCCCAGACCACCCCACACCGGACGTTT
GGGGGAGGGAAAGCAGCAGTGGTTCTCCTGGCTGTGGGGGGACAGTTCCTGCTCTGTTGGTTGCCCTACT
TCTCTTTCCACCTCTATGTTGCCCTGAGTGCTCAGCCCATTTCAACTGGGCAGGTGGAGAGTGTGGTCAC
CTGGATTGGCTACTTTTGCTTCACTTCCAACCCTTTCTTCTATGGATGTCTCAACCGGCAGATCCGGGGG
GAGCTCAGCAAGCAGTTTGTCTGCTTCTTCAAGCCAGCTCCAGAGGAGGAGCTGAGGCTGCCTAGCCGGG
AGGGCTCCATTGAGGAGAACTTCCTGCAGTTCCTTCAGGGGACTGGCTGTCCTTCTGAGTCCTGGGTTTC
CCGACCCCTACCCAGCCCCAAGCAGGAGCCACCTGCTGTTGACTTTCGAATCCCAGGCCAGATAGCTGAG
GAGACCTCTGAGTTCCTGGAGCAGCAACTCACCAGCGACATCATCATGTCAGACAGCTACCTCCGTCCTG
CCGCCTCACCCCGGCTGGAGTCATGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.