Human GPR183/EBI2/hEBI2 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
GPR183 (G-protein coupled receptor 183) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family. GPR183 was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. GPR183 is most closely related to the thrombin receptor. It is expressed in lymphocytes to act as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. GPCR183 is a receptor for oxysterol 7-alpha, 25-dihydroxycholesterol (7-alpha, 25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. GPR183 collaborates with CXCR5 to mediate B-cell migration. GPR183 also acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4+ dendritic cells. GPR183 regulates migration of astrocytes and promotes osteoclast precursor migration to bone surfaces.
Gene Symbol: GPR183; EBI2; hEBI2
NCBI Gene ID: 1880
Uniprot Entry: P32249
Construct Details: Full length human GPR183 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 1086 (bp)
Gene Insert Sequence:
ATGGATATACAAATGGCAAACAATTTTACTCCGCCCTCTGCAACTCCTCAGGGAAATGACTGTGACCTCT
ATGCACATCACAGCACGGCCAGGATAGTAATGCCTCTGCATTACAGCCTCGTCTTCATCATTGGGCTCGT
GGGAAACTTACTAGCCTTGGTCGTCATTGTTCAAAACAGGAAAAAAATCAACTCTACCACCCTCTATTCA
ACAAATTTGGTGATTTCTGATATACTTTTTACCACCGCTTTGCCTACACGAATAGCCTACTATGCAATGG
GCTTTGACTGGAGAATCGGAGATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTACATCAACACATATGC
AGGTGTGAACTTTATGACCTGCCTGAGTATTGACCGCTTCATTGCTGTGGTGCACCCTCTACGCTACAAC
AAGATAAAAAGGATTGAACATGCAAAAGGCGTGTGCATATTTGTCTGGATTCTAGTATTTGCTCAGACAC
TCCCACTCCTCATCAACCCTATGTCAAAGCAGGAGGCTGAAAGGATTACATGCATGGAGTATCCAAACTT
TGAAGAAACTAAATCTCTTCCCTGGATTCTGCTTGGGGCATGTTTCATAGGATATGTACTTCCACTTATA
ATCATTCTCATCTGCTATTCTCAGATCTGCTGCAAACTCTTCAGAACTGCCAAACAAAACCCACTCACTG
AGAAATCTGGTGTAAACAAAAAGGCTCTCAACACAATTATTCTTATTATTGTTGTGTTTGTTCTCTGTTT
CACACCTTACCATGTTGCAATTATTCAACATATGATTAAGAAGCTTCGTTTCTCTAATTTCCTGGAATGT
AGCCAAAGACATTCGTTCCAGATTTCTCTGCACTTTACAGTATGCCTGATGAACTTCAATTGCTGCATGG
ACCCTTTTATCTACTTCTTTGCATGTAAAGGGTATAAGAGAAAGGTTATGAGGATGCTGAAACGGCAAGT
CAGTGTATCGATTTCTAGTGCTGTGAAGTCAGCCCCTGAAGAAAATTCACGTGAAATGACAGAAACGCAG
ATGATGATACATTCCAAGTCTTCAAATGGAAAGTGA
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.