Human GPR149/R35/IEDA/PGR10 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA
GPR149 (G-protein coupled receptor 149) is a 7 transmembrane receptor belonging to the G protein-coupled receptor 1 (GPCR1) family. GPR149 is an orphan GPCR with unknown function.
Gene Symbol: GPR149; R35; IEDA; PGR10
NCBI Gene ID: 344758
Uniprot Entry: Q86SP6
Construct Details: Full length human GPR149 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2196 (bp)
Gene Insert Sequence:
ATGTCTTTATTTCTCAGTAACTTATCAACAAATGACTCTAGCCTGTGGAAAGAGAATCATAATTCTACGG
ACCTTTTAAATCCGCCAGGAACCCTGAATATCTATCTTTTTTGCTTGACATGTCTCATGACTTTTGCAGC
CTTGGTGGGCAGCATTTATTCACTAATTTCCCTGCTGAAAATGCAGAACAGAACTGTTGTGTCCATGCTT
GTGGCTTCCTGGTCTGTGGATGATCTCATGAGCGTCCTGTCGGTGACCATCTTCATGTTTTTGCAGTGGC
CAAACGAGGTCCCCGGTTACTTCCAATTTCTGTGCACCACCTCTGCCTTAATGTATTTATGCCAGGGCCT
CTCTAGCAACTTGAAGGCGACTCTCCTAGTCTCTTACAACTTTTATACGATGCACAGAGGTGTGGGGAGC
CAGACAGCCTCCAGAAGATCGGGCCAGGTGCTCGGCGTGGTGCTGACCGTGTGGGCAGCCAGTCTGCTGC
TCTCGGCGCTCCCGCTGTGCGGCTGGGGCGCCTTCGTGCGCACGCCCTGGGGCTGCCTGGTGGACTGCTC
CAGCTCCTACGTACTATTCCTCTCTATCGTGTACGCTTTGGCCTTCGGACTCCTCGTGGGCCTCTCAGTC
CCACTCACTCACCGATTGCTGTGTTCGGAGGAGCCGCCGAGACTCCACTCCAACTACCAGGAAATTTCCC
GTGGAGCTTCAATTCCTGGGACCCCTCCTACTGCGGGGAGAGTGGTTTCCCTGTCCCCAGAGGATGCTCC
AGGCCCGAGTCTGCGGCGCTCTGGGGGATGCTCTCCGAGCTCCGACACCGTGTTCGGACCGGGTGCGCCC
GCTGCCGCTGGGGCTGAAGCCTGCAGGCGTGAGAACCGGGGGACTCTCTATGGCACCAGGAGCTTCACCG
TGAGCGTAGCGCAGAAGCGCTTCGCTTTGATCCTAGCGCTTACAAAAGTCGTCCTTTGGCTGCCCATGAT
GATGCACATGGTGGTCCAGAACGTCGTGGGGTTTCAGAGCCTTCCCTTGGAGACATTCAGCTTTCTACTT
ACCCTGCTGGCCACCACTGTAACCCCAGTGTTTGTCTTGTCCAAACGCTGGACCCACTTGCCCTGTGGCT
GCATCATCAACTGCAGGCAGAACGCATATGCAGTGGCGTCCGATGGGAAAAAAATCAAGAGAAAAGGCTT
TGAATTCAATCTATCATTCCAAAAAAGTTATGGGATTTATAAAATAGCACATGAAGATTACTATGATGAT
GATGAAAATTCCATATTCTATCACAACCTGATGAACTCTGAGTGTGAAACTACAAAAGACCCTCAGAGAG
ACAACCGTAACATCTTCAATGCTATAAAAGTAGAAATCAGCACCACGCCCTCTCTGGACAGCTCCACACA
AAGAGGCATCAACAAATGCACAAATACTGATATTACAGAAGCTAAACAGGATTCCAACAACAAAAAGGAT
GCGTTTTCTGACAAAACAGGAGGTGATATTAACTATGAAGAAACTACCTTTTCTGAAGGGCCAGAAAGAA
GACTGTCTCATGAAGAGAGTCAGAAACCAGATCTTTCAGACTGGGAGTGGTGTAGGAGTAAATCAGAAAG
AACCCCTCGTCAGCGTTCCGGTTATGCCCTTGCCATTCCCTTGTGTGCATTCCAGGGGACTGTGTCTCTC
CATGCACCTACAGGGAAAACCCTATCTCTTTCTACCTATGAGGTAAGCGCAGAAGGGCAAAAAATAACTC
CAGCCTCTAAGAAAATAGAAGTCTATCGATCCAAAAGTGTTGGCCATGAACCAAACTCAGAAGATTCTTC
ATCCACGTTTGTGGACACCAGTGTGAAAATACACTTGGAGGTTCTTGAAATTTGTGATAATGAAGAGGCC
TTGGACACTGTGTCAATCATTAGTAACATCAGTCAGTCCTCCACACAAGTCAGATCTCCATCCCTACGTT
ACTCCAGGAAAGAAAACAGATTTGTTTCATGTGACCTAGGGGAAACAGCCTCATACTCCCTCTTTTTGCC
CACCAGTAATCCTGATGGTGATATTAATATCTCCATTCCAGACACAGTAGAAGCACACAGGCAGAACAGT
AAAAGGCAGCATCAAGAGAGGGATGGCTACCAGGAGGAAATCCAGTTGTTAAATAAAGCTTACAGAAAAA
GAGAGGAAGAAAGCAAGGGTAGTTAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.