Human CD30 Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-CD30 (SKU#: LTP0815)

Price:

$1,096.00

Description

CD30/TNFRSF8 is a single-pass type I transmembrane glycoprotein of the TNF receptor superfamily (TNFRSF). The ligand for CD30 is CD30L/CD153, a member of the TNF superfamily (TNFSF8). CD30L-CD30 ligation mediates pleiotropic effects, including cell proliferation, activation, differentiation and apoptosis. CD30 is expressed in activated, but not resting, T and B cells. CD30 can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus (HIV) replication. As a regulator of apoptosis, CD30 protein induces cell death or proliferation, depending on the cell type, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells. In B cells, CD30 ligation promotes cellular proliferation and antibody production. CD30 is overexpressed in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs). CD30 is also found in leukocytes in patients with chronic inflammatory diseases, including lupus erythematosus, asthma, rheumatoid arthritis and atopic dermatitis.

Gene Symbol: CD30; Ki-1; TNFRSF8; D1S166E

NCBI Gene ID: 943

Uniprot Entry: P28908

Construct Details: Full length human CD30 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1788 (bp)

Gene Insert Sequence:

ATGCGCGTCCTCCTCGCCGCGCTGGGACTGCTGTTCCTGGGGGCGCTACGAGCCTTCCCACAGGATCGACCCTTCGAGGA

CACCTGTCATGGAAACCCCAGCCACTACTATGACAAGGCTGTCAGGAGGTGCTGTTACCGCTGCCCCATGGGGCTGTTCC

CGACACAGCAGTGCCCACAGAGGCCTACTGACTGCAGGAAGCAGTGTGAGCCTGACTACTACCTGGATGAGGCCGACCGC

TGTACAGCCTGCGTGACTTGTTCTCGAGACGACCTCGTGGAGAAGACGCCGTGTGCATGGAACTCCTCCCGTGTCTGCGA

ATGTCGACCCGGCATGTTCTGTTCCACGTCTGCCGTCAACTCCTGTGCCCGCTGCTTCTTCCATTCTGTCTGTCCGGCAG

GGATGATTGTCAAGTTCCCAGGCACGGCGCAGAAGAACACGGTCTGTGAGCCGGCTTCCCCAGGGGTCAGCCCTGCCTGT

GCCAGCCCAGAGAACTGCAAGGAACCCTCCAGTGGCACCATCCCCCAGGCCAAGCCCACCCCGGTGTCCCCAGCAACCTC

CAGTGCCAGCACCATGCCTGTAAGAGGGGGCACCCGCCTCGCCCAGGAAGCTGCTTCTAAACTGACGAGGGCTCCCGACT

CTCCCTCCTCTGTGGGAAGGCCTAGTTCAGATCCAGGTCTGTCCCCAACACAGCCATGCCCAGAGGGGTCTGGTGATTGC

AGAAAGCAGTGTGAGCCCGACTACTACCTGGACGAGGCCGGCCGCTGCACGGCCTGCGTGAGCTGTTCTCGAGATGACCT

TGTGGAGAAGACGCCATGTGCATGGAACTCCTCCCGCACCTGCGAATGTCGACCTGGCATGATCTGTGCCACATCAGCCA

CCAACTCCCGTGCCCGCTGTGTCCCCTACCCAATCTGTGCAGCAGAGACGGTCACCAAGCCCCAGGATATGGCTGAGAAG

GACACCACCTTTGAGGCGCCACCCCTGGGGACCCAGCCGGACTGCAACCCCACCCCAGAGAATGGCGAGGCGCCTGCCAG

CACCAGCCCCACTCAGAGCTTGCTGGTGGACTCCCAGGCCAGTAAGACGCTGCCCATCCCAACCAGCGCTCCCGTCGCTC

TCTCCTCCACGGGGAAGCCCGTTCTGGATGCAGGGCCAGTGCTCTTCTGGGTGATCCTGGTGTTGGTTGTGGTGGTCGGC

TCCAGCGCCTTCCTCCTGTGCCACCGGAGGGCCTGCAGGAAGCGAATTCGGCAGAAGCTCCACCTGTGCTACCCGGTCCA

GACCTCCCAGCCCAAGCTAGAGCTTGTGGATTCCAGACCCAGGAGGAGCTCAACGCAGCTGAGGAGTGGTGCGTCGGTGA

CAGAACCCGTCGCGGAAGAGCGAGGGTTAATGAGCCAGCCACTGATGGAGACCTGCCACAGCGTGGGGGCAGCCTACCTG

GAGAGCCTGCCGCTGCAGGATGCCAGCCCGGCCGGGGGCCCCTCGTCCCCCAGGGACCTTCCTGAGCCCCGGGTGTCCAC

GGAGCACACCAATAACAAGATTGAGAAAATCTACATCATGAAGGCTGACACCGTGATCGTGGGGACCGTGAAGGCTGAGC

TGCCGGAGGGCCGGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAGGAGGAGCTGGAGGCGGACCATACCCCCCACTAC

CCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGCAGCGATGTCATGCTCTCAGTGGAAGAGGAAGGGAAAGAAGACCC

CTTGCCCACAGCTGCCTCTGGAAAGTGA

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

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3. Proc. Natl. Acad. Sci. 93 (24): 14053 (1996).

4. J. Biol. Chem. 271 (22): 12852 (1996).

5. Immunology 118:143 (2006).

Product datasheet (pdf) can be downloaded here: LTP0815-PDS.pdf