Human VSTM1/SIRL1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

VSTM1 (V-set and transmembrane domain-containing protein 1) or SIRL1 (Signal inhibitory receptor on leukocytes-1) is a single pass type I transmembrane protein belonging to the Immunoglobulin superfamily (IgSF).  VSTM1 contains a 119 aa extracellular region (ECD) with 1 V-type Ig­-like (IgV) domain, a transmembrane (TM) domain and a 80 aa cytoplasmic tail with 2 copies of immunoreceptor tyrosine-based inhibitor motif (ITIM). The ITIM is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. Both motives are required for full inhibition of FCER1A-mediated degranulation.  VSTM1 is expressed on myeloid (neutrophils, eosinophils and monocytes) but not on lymphoid cells.  VSTM1 may act as an inhibitory immune receptor involved in the regulation of phagocytes.  The Isoform 2 of VSTM1 (v2) which lacks the TM domain behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17).

 

 

Gene Symbol: VSTM1; SIRL1; SIRL-1; VSTM-1; UNQ3033

 

NCBI Gene ID: 284415

 

Uniprot Entry: Q6UX27

 

Construct Details: Full length human VSTM1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 711 (bp)

 

Gene Insert Sequence:  

ATGACCGCAGAATTCCTCTCCCTGCTTTGCCTCGGGCTGTGTCTGGGCTACGAAGATGAGAAAAAGAATG

AGAAACCGCCCAAGCCCTCCCTCCACGCCTGGCCCAGCTCGGTGGTTGAAGCCGAGAGCAATGTGACCCT

GAAGTGTCAGGCTCATTCCCAGAATGTGACATTTGTGCTGCGCAAGGTGAACGACTCTGGGTACAAGCAG

GAACAGAGCTCGGCAGAAAACGAAGCTGAATTCCCCTTCACGGACCTGAAGCCTAAGGATGCTGGGAGGT

ACTTTTGTGCCTACAAGACAACAGCCTCCCATGAGTGGTCAGAAAGCAGTGAACACTTGCAGCTGGTGGT

CACAGATAAACACGATGAACTTGAAGCTCCCTCAATGAAAACAGACACCAGAACCATCTTTGTCGCCATC

TTCAGCTGCATCTCCATCCTTCTCCTCTTCCTCTCAGTCTTCATCATCTACAGATGCAGCCAGCACAGTT

CATCATCTGAGGAATCCACCAAGAGAACCAGCCATTCCAAACTTCCGGAGCAGGAGGCTGCCGAGGCAGA

TTTATCCAATATGGAAAGGGTATCTCTCTCGACGGCAGACCCCCAAGGAGTGACCTATGCTGAGCTAAGC

ACCAGCGCCCTGTCTGAGGCAGCTTCAGACACCACCCAGGAGCCCCCAGGATCTCATGAATATGCGGCAC

TGAAAGTGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.