Human VSIG10L Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

VSIG10L (V-set and immunoglobulin domain-containing protein 10-like) is a single pass type I transmembrane protein belonging to the Immunoglobulin superfamily (IgSF).  VSIG10L contains a 749 aa extracellular region (ECD) with 2 C2-type Ig­-like (IgC2) domains, a transmembrane (TM) domain and a 70 aa cytoplasmic tail. VSIG10L contains multiple glycosylation sites at the N-terminal ECD region.  The function of VSIG10L is unclear.

 

 

Gene Symbol: VSIG10L

 

NCBI Gene ID: 147645

 

Uniprot Entry: Q86VR7

 

Construct Details: Full length human VSIG10L gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2604 (bp)

 

Gene Insert Sequence:  

ATGGACAACCCACAGGCTCTGCCACTCTTCCTACTCCTGGCCTCCTTGGTAGGGATCCTCACCCTCAGAG

CCTCTTCTGGACTTCAGCAAACCAACTTCTCCTCTGCCTTCTCTTCAGACTCAAAGAGCTCTTCCCAGGG

GCTGGGTGTGGAAGTTCCCTCCATCAAACCTCCCAGCTGGAAAGTTCCAGATCAGTTCCTGGATTCAAAA

GCCTCTGCTGGAATCTCTGATTCCAGCTGGTTTCCTGAGGCCCTGAGTTCCAACATGTCTGGGTCCTTCT

GGTCAAATGTTTCTGCTGAGGGCCAAGATTTGAGCCCGGTTTCCCCCTTCTCTGAAACCCCTGGTTCTGA

AGTATTTCCTGATATTTCGGATCCTCAAGTTCCTGCCAAAGACCCCAAGCCTTCCTTCACTGTTAAGACC

CCAGCTTCAAACATTTCTACTCAAGTCTCCCATACCAAACTGTCTGTTGAGGCCCCAGATTCAAAATTCT

CCCCGGATGATATGGATCTTAAACTCTCTGCCCAGAGCCCTGAATCCAAATTTTCTGCAGAGACCCACTC

AGCTGCAAGCTTTCCCCAGCAGGTGGGGGGCCCACTCGCTGTGCTGGTGGGGACCACCATCCGGCTCCCC

CTAGTCCCAATCCCCAACCCTGGGCCCCCCACCTCTCTGGTGGTCTGGCGCCGGGGCTCAAAGGTGCTGG

CAGCTGGGGGCCTGGGGCCAGGGGCACCTCTGATCAGCCTGGACCCTGCTCACCGAGACCACCTGCGATT

TGACCAGGCCCGGGGGGTTCTGGAGCTCGCCTCTGCCCAGCTGGACGATGCAGGGGTCTACACGGCTGAG

GTCATCCGGGCAGGGGTCTCCCAGCAGACTCACGAGTTCACGGTGGGTGTGTATGAGCCCCTACCCCAGC

TGTCGGTTCAGCCCAAGGCTCCAGAGACAGAGGAGGGGGCGGCCGAGCTCCGGCTGCGCTGCCTGGGGTG

GGGGCCAGGTCGCGGGGAGCTGAGCTGGAGCCGGGACGGACGCGCCCTGGAGGCGGCGGAATCGGAGGGA

GCCGAGACGCCCCGGATGCGCTCAGAGGGCGACCAGCTGCTCATCGTGCGCCCTGTGCGCAGCGACCACG

CCCGGTACACTTGCCGCGTCCGCAGCCCCTTCGGCCACAGGGAGGCTGCCGCCGACGTCAGCGTCTTCTA

CGGCCCGGACCCGCCGACCATCACGGTCTCCTCGGACCGCGACGCCGCGCCTGCCCGCTTTGTCACCGCG

GGCAGTAACGTGACCTTGCGCTGCGCCGCCGCCTCGCGGCCGCCCGCCGACATCACGTGGAGCCTGGCGG

ACCCGGCCGAGGCCGCGGTGCCCGCGGGGTCGCGCCTCCTGCTGCCCGCGGTCGGACCGGGCCACGCAGG

CACCTACGCCTGCCTGGCGGCGAACCCGCGTACCGGCCGCCGCCGCCGCTCGCTGCTCAACCTTACAGTG

GCGGACCTGCCCCCCGGGGCCCCACAGTGCTCAGTTGAAGGGGGTCCCGGGGACCGCAGCCTCCGCTTCC

GCTGCTCGTGGCCCGGCGGGGCCCCTGCTGCCTCCCTGCAGTTCCAGGGTCTCCCCGAAGGCATCCGCGC

CGGGCCAGTGTCCTCTGTGCTGCTGGCGGCCGTCCCCGCCCACCCCCGGCTCAGCGGCGTCCCCATCACC

TGCCTTGCTCGCCACCTGGTGGCCACGCGTACCTGCACAGTCACGCCGGAGGCCCCCCGAGAGGTGCTGC

TGCATCCGCTGGTGGCAGAGACACGGTTGGGGGAGGCAGAGGTGGCACTGGAGGCCTCTGGTTGTCCCCC

ACCCTCACGGGCATCCTGGGCCCGGGAAGGGAGGCCCCTGGCTCCAGGAGGCGGGAGTCGCCTGCGGCTC

AGTCAAGATGGGCGGAAACTCCACATCGGCAACTTCAGCCTGGATTGGGACCTGGGAAATTACTCCGTGC

TGTGCAGTGGGGCGCTGGGTGCTGGCGGTGACCAGATCACCCTCATTGGACCCTCCATATCCTCGTGGAG

GCTTCAGAGAGCCAGAGATGCAGCCGTGCTGACTTGGGATGTGGAGCGCGGGGCCCTGATCAGCAGTTTT

GAGATCCAGGCATGGCCAGATGGGCCTGCTCTGGGCAGGACTTCCACCTACAGGGACTGGGTCTCCCTGC

TCATCCTGGGGCCTCAGGAGCGGTCAGCCGTGGTGCCCCTTCCACCTCGGAACCCAGGGACCTGGACCTT

TCGGATCCTGCCCATCCTGGGGGGCCAGCCAGGGACTCCATCACAAAGCCGGGTCTACCGGGCCGGCCCC

ACGTTGAGCCATGGGGCCATCGCTGGCATCGTCCTGGGCTCCCTGCTGGGCCTGGCGCTGCTAGCCGTAC

TTCTCCTCCTTTGCATCTGCTGCCTGTGCCGCTTTCGTGGAAAGACTCCTGAGAAAAAGAAGCATCCTTC

TACCTTGGTCCCCGTGGTCACCCCCTCAGAAAAGAAGATGCATAGTGTGACCCCAGTGGAGATTTCATGG

CCTCTGGACCTCAAAGTCCCTCTGGAGGACCACAGCTCAACTAGGGCCTACCAAGCACAGACCCCAGTTC

AGTTGTCTCTGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.