Human CEACAM16/CEAL2/DFNA4B Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CEACAM16 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 16) is a secreted glycoprotein of the CEA family of the immunoglobulin superfamily (IgSF). Members of the CEA family consist of a single N domain, with structural similarity to the Ig variable (IgV) domains, followed by a variable number of Ig constant-like 1 (IgC1) and/or 2 (IgC2) domains. Human CEACAM16 contains 2 C2-type (IgC2) domains with multiple sites for glycosylation. CEACAM16 is localized to the tip of cochlear outer hair cells and to the tectorial membrane.  CEACAM16 is required for proper hearing with a role in maintaining the integrity of the tectorial membrane. Defects in the CEACAM16 gene likely are a cause of non-syndromic autosomal dominant hearing loss, 4B (DFNA4B).

 

 

Gene Symbol: CEACAM16; CEAL2; CEAL-2; DFNA4B

 

NCBI Gene ID: 388551

 

Uniprot Entry: Q2WEN9

 

Construct Details: Full length human CEACAM16 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1278 (bp)

 

Gene Insert Sequence:  

ATGGCGCTGACTGGGTACAGCTGGCTGCTCCTCAGTGCCACATTCCTGAATGTGGGGGCCGAGATCTCTA

TCACCCTGGAGCCTGCCCAGCCGAGCGAAGGGGACAACGTCACGCTGGTCGTCCATGGGCTTTCGGGGGA

ACTGCTCGCCTACAGCTGGTATGCGGGGCCCACACTCAGCGTGTCATACCTGGTGGCCAGCTACATCGTG

AGCACAGGCGATGAGACTCCTGGCCCGGCCCACACGGGGCGGGAGGCTGTGCGCCCCGATGGCAGCCTGG

ACATCCAGGGCATCCTGCCCCGGCACTCAGGCACCTACATCCTGCAGACCTTCAACAGGCAGTTGCAGAC

CGAGGTGGGCTACGGACACGTGCAGGTCCATGAGATCCTGGCCCAGCCCACAGTCTTGGCCAACAGCACA

GCGCTGGTGGAACGTCGAGACACCCTGCGCCTTATGTGCAGCAGCCCCAGCCCCACCGCCGAGGTCCGCT

GGTTCTTCAACGGTGGGGCCCTGCCCGTCGCTCTCCGCCTGGGCCTGTCCCCTGACGGCCGGGTGCTGGC

CAGGCATGGCATCCGCCGGGAGGAGGCCGGCGCCTATCAGTGTGAGGTGTGGAACCCGGTCAGTGTCAGC

CGCAGCGAGCCCATCAACCTGACCGTGTACTTTGGCCCAGAGCGTGTGGCCATCCTCCAGGATTCCACCA

CCCGCACAGGCTGCACCATCAAAGTTGACTTCAACACGTCCCTCACCCTGTGGTGCGTGTCCAGGTCCTG

CCCAGAGCCCGAGTATGTGTGGACCTTCAACGGGCAGGCCCTAAAGAACGGCCAAGACCACCTCAACATC

AGCAGCATGACAGCCGCCCAGGAGGGGACGTACACATGTATTGCGAAGAACACCAAGACCCTGCTATCTG

GATCTGCCTCAGTCGTGGTCAAGCTCTCTGCTGCAGCAGTTGCCACGATGATCGTGCCCGTGCCCACCAA

GCCAACGGAGGGCCAGGACGTAACACTGACCGTGCAGGGCTACCCCAAGGACCTGCTGGTCTACGCCTGG

TACCGCGGGCCTGCCTCCGAGCCCAACCGGCTGCTCAGCCAGCTGCCGTCAGGAACCTGGATTGCAGGCC

CCGCGCACACAGGCCGGGAGGTGGGCTTCCCCAACTGCTCGCTGTTGGTGCAGAAGCTGAACCTCACAGA

CACTGGCCGCTACACACTCAAGACTGTCACAGTGCAGGGCAAGACTGAGACACTGGAAGTGGAGCTGCAG

GTGGCCCCCCTGGGGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.