Human CEACAM4/CGM7/NCA Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CEACAM4 (carcinoembryonic antigen (CEA)-related cell adhesion molecule 4) and Carcinoembryonic antigen CGM7, is a single pass type I transmembrane protein of the CEA family of the immunoglobulin superfamily (IgSF). CEA family members are either transmembrane or GPI­-anchored within two subgroups: the CEACAMs and the pregnancy-specific glycoproteins that are used by several bacterial pathogens to bind and invade host cells.  Members of the CEA family consist of a single N domain, with structural similarity to the Ig variable (IgV) domains, followed by a variable number of Ig constant-like 1 (IgC1) and/or 2 (IgC2) domains.  In human, 7 CEACAMs have been identified including 3 transmembrane proteins (CEACAM1, 3, 4) and 4 GPI-anchored molecules (CEACAM5-8). Human CEACAM4 contains 1 Ig-like V-type (IgV) motif in the N-terminal 120 aa extracellular domain (ECD), followed by a transmembrane domain (TM) and a 68 aa cytoplasmic tail with the ITAM-like sequence. The ITAM becomes tyrosine phosphorylated by SRC family PTKs upon ligand-mediated receptor clustering and allows to initiate phagocytosis of bound ligand.  CEACAM4 is a Granulocyte orphan receptor that acts as an trigger efficient phagocytosis of attached particles. 

 

 

Gene Symbol: CEACAM4; NCA; CGM7; W236

 

NCBI Gene ID: 1089

 

Uniprot Entry: O75871

 

Construct Details: Full length human CEACAM4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 735 (bp)

 

Gene Insert Sequence:  

ATGGGCCCCCCCTCAGCCGCTCCCCGTGGAGGGCACAGGCCCTGGCAGGGGCTCCTGATCACAGCCTCAC

TTTTAACCTTCTGGCACCCGCCCACCACTGTCCAGTTCACTATTGAAGCCCTGCCGTCCAGTGCTGCAGA

GGGAAAGGATGTTCTTCTACTGGCCTGCAATATTTCAGAAACTATTCAAGCCTATTATTGGCACAAGGGG

AAAACGGCAGAAGGGAGCCCTCTCATTGCTGGTTATATAACAGACATTCAAGCAAATATCCCAGGGGCCG

CATACAGTGGTCGAGAGACAGTATACCCCAATGGATCCCTGCTGTTCCAAAACATCACCCTGGAGGACGC

AGGATCCTACACCCTACGAACCATAAATGCCAGTTACGACTCTGACCAAGCAACTGGCCAGCTCCACGTA

CACCAAAACAACGTCCCAGGCCTTCCTGTGGGGGCCGTCGCTGGCATCGTGACTGGGGTCCTGGTTGGGG

TGGCTCTGGTGGCCGCCCTGGTGTGTTTTCTGCTTCTCTCCAGGACTGGAAGGGCCAGCATCCAGCGTGA

CCTCAGGGAGCAGCCGCCCCCAGCCTCCACCCCTGGCCATGGTCCCTCTCACAGATCCACCTTCTCGGCC

CCTCTACCCAGCCCCAGAACAGCCACTCCCATCTATGAGGAATTGCTATACTCTGATGCAAACATTTACT

GCCAGATCGACCACAAAGCAGATGTGGTCTCTTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.