Human C7orf15/PVRIG/CD112R Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

C7orf15 (chromosome 7 open reading frame 15) also know as PVRIG (Poliovirus receptor-related immunoglobulin domain-containing protein) and CD112R may encode a 3-pass transmembrane protein. It was named PVRIG for the homology observed between its second exon and the variable immunoglobulin (Ig) domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes.  C7orf15 is expressed in some types of immune cells. It is expressed at low levels on the surface of freshly isolated T-cells and natural killer (NK) cells but not detected in B-cells, naive or helper T-cells, monocytes, nor neutrophils. Its expression in T cells is up-regulated by activation.  C7orf15 is the cell surface receptor for NECTIN2 and competes with CD226 for NECTIN2-binding.  It may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, C7orf15 inhibits T-cell proliferation.

 

 

Gene Symbol: C7orf15; PVRIG; CD112R

 

NCBI Gene ID: 79037

 

Uniprot Entry: Q6DKI7

 

Construct Details: Full length human PVRIG gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 981 (bp)

 

Gene Insert Sequence:  

ATGAGAACAGAGGCACAGGTGCCGGCCCTGCAGCCCCCAGAACCTGGACTGGAGGGGGCCATGGGGCACC

GGACCCTGGTCCTGCCCTGGGTGCTGCTGACCTTGTGTGTCACTGCGGGGACCCCGGAGGTGTGGGTTCA

AGTTCGGATGGAGGCCACCGAGCTCTCGTCCTTCACCATCCGTTGTGGGTTCCTGGGGTCTGGCTCCATC

TCCCTGGTGACTGTGAGCTGGGGGGGCCCCAACGGTGCTGGGGGGACCACGCTGGCTGTGTTGCACCCAG

AACGTGGCATCCGGCAATGGGCCCCTGCTCGCCAGGCCCGCTGGGAAACCCAGAGCAGCATCTCTCTCAT

CCTGGAAGGCTCTGGGGCCAGCAGCCCCTGCGCCAACACCACCTTCTGCTGCAAGTTTGCGTCCTTCCCT

GAGGGCTCCTGGGAGGCCTGTGGGAGCCTCCCGCCCAGCTCAGACCCAGGGCTCTCTGCCCCGCCGACTC

CTGCCCCCATTCTGCGGGCAGACCTGGCCGGGATCTTGGGGGTCTCAGGAGTCCTCCTCTTTGGCTGTGT

CTACCTCCTTCATCTGCTGCGCCGACATAAGCACCGCCCTGCCCCTAGGCTCCAGCCGTCCCGCACCAGC

CCCCAGGCACCGAGAGCACGAGCATGGGCACCAAGCCAGGCCTCCCAGGCTGCTCTTCACGTCCCTTATG

CCACTATCAACACCAGCTGCCGCCCAGCTACTTTGGACACAGCTCACCCCCATGGGGGGCCGTCCTGGTG

GGCGTCACTCCCCACCCACGCTGCACACCGGCCCCAGGGCCCTGCCGCCTGGGCCTCCACACCCATCCCT

GCACGTGGCAGCTTTGTCTCTGTTGAGAATGGACTCTACGCTCAGGCAGGGGAGAGGCCTCCTCACACTG

GTCCCGGCCTCACTCTTTTCCCTGACCCTCGGGGGCCCAGGGCCATGGAAGGACCCTTAGGAGTTCGATG

A

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.