Human C1orf101/CATSPERE Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA
C1orf101 (chromosome 1 open reading frame 101) also known as CATSPERE (Cation channel sperm-associated protein subunit epsilon) is a single-pass type I transmembrane protein. C1orf101 may be an auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization.
Gene Symbol: C1orf101; CATSPERE
NCBI Gene ID: 257044
Uniprot Entry: Q5SY80
Construct Details: Full length human C1orf101 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 2856 (bp)
Gene Insert Sequence:
ATGTCAGCCCGGGAAGTGGCCGTGCTGCTGCTGTGGCTGAGCTGCTATGGCTCCGCCCTTTGGAGGTATT
CCACTAACAGCCCAAACTATCGCATTTTTAGTACCAGAAGTACTATTAAGTTAGAGTATGAAGGAACATT
ATTTACTGAGTGGAGTGTGCCAGAAACTTGTTTTGTGCTAAATAAAAGCTCACCCACGACAGAATTGCGT
TGTTCCTCACCTGGTGTTCACGCTATAAAACCAATTGTTACTGGCCCAGATGAAGAAGAACGCTATTTAT
TTGTGGAAAGTTCTCATACTTGCTTTCTGTGGTACTATAGAGTTAGACATTTCTTTAACAACTTTACCCA
GCTTATCACTGTGTGGGCATATGATCCAGAAAGTGCAGATCCTGATGAGTTGCTGGGGAATGCAGAAGAA
CCTTCAATAAATTCCATAGTACTCAGCACACAGATGGCCACATTGGGACAGAAGCCTGTCATACATACAG
TTCTGAAGAGAAAAGTTTATTCTTCAAATGAGAAAATGAGAAGGGGGACCTGGCGTATTGTAGTACCAAT
GACAAAAGATGATGCACTAAAGGAGATTAGAGGAAACCAAGTTACTTTTCAGGATTGCTTTATTGCAGAT
TTTCTTATTCTGTTGACTTTTCCTTTGTTGACCATACCTGAAATTCCTGGTTATTTACCAATCTCCTCAC
CACGTGGTAGTCAATTAATGGCTTCCTGGGATGCTTGTGTAGTTGCATCTGCTGTTTTGGTGACAGATAT
GGAGACCTTTCACACAACTGATTCATTCAAATCTTGGACCAGAATCAGAGTGCCTCCAGACATTCTGAGT
GATGATGAAAGACGGAGTGTGGCTCATGTGATCTTATCGCGGGATGGAATCGTTTTTCTTATAAATGGTG
TTCTTTACATAAAGAGTTTTCGTGGATTTATAAGACTGGGAGGAATTGTAAATCTTCCTGATGGTGGAAT
TACTGGCATTTCATCAAGAAAATGGTGTTGGGTCAATTATTTATTAAAGGCTAAAGGAAGAAGAAGCACC
TTTGCAGTCTGGACAGAAAATGAAATTTACCTCGGATCCATTCTTCTTAAGTTTGCCAGATTAGTAACTA
CCACAGAACTGAAAAACATCCTAAGTCTATCGGTGACTGCTACTCTGACCATAGACAGGGTTGAGTATAC
AGGACACCCTCTGGAGATTGCTGTGTTTTTAAATTATTGCACTGTATGTAACGTCACCAAAAAGATTTTC
TTAGTGATATATAATGAAGATACAAAACAGTGGGTTTCCCAAGACTTTACATTAGATGCCCCTATTGACA
GTGTTACCATGCCACATTTTACATTTTCAGCACTGCCAGGATTACTGCTATGGAACAAGCATAGTATCTA
CTATTGTTACCATAATTTCACCTTTACTGGGATTTTACAGACACCTGCAGGACATGGAAATCTATCAATG
CTATCAAATGACAGCATTATTCATGAAGTTTTCATAGATTATTATGGAGATATTTTGGTAAAAATGGAAA
ATAATGTAATATTTTATTCCAAGATTAATACTAGAGATGCAGTAAAGCTGCATTTATGGACAAATTACAC
AACAAGAGCATTCATTTTCTTAAGTACATCTGGTCAAACATATTTCCTGTATGCTTTGGATGATGGCACA
ATACAAATACAGGACTATCCCTTACATCTGGAAGCACAAAGTATAGCTTTCACAACAAAAGACAAATGCC
CATACATGGCATTTCATAACAATGTTGCTCATGTTTTTTACTTTTTGGACAAGGGAGAGGCTCTGACAGT
TTGGACTCAGATCGTCTATCCAGAAAACACTGGTCTGTATGTTATTGTGGAATCTTATGGCCCAAAAATA
TTACAAGAGAGTCATGAGATTTCCTTTGAAGCTGCCTTTGGATACTGCACCAAAACTCTGACACTAACAT
TTTATCAGAATGTAGATTATGAGAGAATATCTGATTACTTTGAGACACAAGACAAGCACACGGGTCTTGT
GCTGGTTCAGTTTCGACCTAGTGAATATTCAAAAGCATGTCCAATAGCCCAAAAGGTGTTCCAAATAGCT
GTTGGCTGTGATGATAAAAAATTCATTGCAATTAAAGGATTTAGTAAAAAAGGATGTCATCACCATGATT
TTTCATACGTGATTGAAAAGTCATATCTGAGGCATCAGCCATCGAAAAACTTGAGAGTAAGGTATATTTG
GGGAGAATATGGCTGCCCTCTGAGGCTTGACTTCACAGAAAAGTTTCAACCTGTGGTTCAACTATTTGAT
GATAATGGCTATGTTAAAGACGTTGAAGCAAATTTCATAGTGTGGGAAATACACGGCAGGGATGACTATA
GCTTTAATAATACTATGGCACAGAGTGGTTGTTTACATGAAGCACAGACATGGAAGTCAATGATTGAACT
TAACAAGCACCTCCCACTAGAAGAAGTCTGGGGACCTGAGAACTATAAACACTGTTTTTCTTATGCTATT
GGAAAACCAGGAGACTTAAATCAACCATACGAGATTATCAACAGTTCTAATGGTAACCATATATTTTGGC
CCATGGGCCATTCTGGAATGTATGTATTTCGTGTGAAGATCCTGGATCCAAACTATAGTTTCTGTAACCT
AACAGCTATGTTTGCAATAGAGACATTTGGACTGATTCCCAGTCCAAGTGTCTACCTGGTAGCTTCTTTC
CTCTTCGTCCTGATGCTGCTCTTCTTCACTATTCTTGTTTTGAGCTACTTTCGGTACATGAGGATTTATA
GACGATATATTTATGAACCACTTCACAAACCTCAAAGAAAACGTAAGAAGAATTAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.