Human IL22RA1/IL22R/CRF2-9 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

IL22RA1 (interleukin-22 receptor subunit alpha-1) is a single-pass type I transmembrane protein belonging to the type II cytokine receptor family.  IL22RA1 can form a heterodimer with IL10RB and with IL20RB. IL22 receptor is a protein complex that consists of IL22RA1 and IL10RB, a subunit also shared by the receptor complex for IL10. IL22 binding to the IL22RA:IL10RB heterodimer is greater than binding to IL22RA1 alone. The IL22RA1 and IL10RB dimer mediates IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. The IL22RA1 and IL20RB dimer is the receptor for IL20 and IL24 which  also signal through JAK/STAT activation. IL22RA1 mediates IL24 anti-angiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.

 

 

Gene Symbol: IL22RA1; IL22R; CRF2-9; IL22R1; ZcytoR11

 

NCBI Gene ID: 58985

 

Uniprot Entry: Q8N6P7

 

Construct Details: Full length human IL22RA1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1725 (bp)

 

Gene Insert Sequence:  

ATGAGGACGCTGCTGACCATCTTGACTGTGGGATCCCTGGCTGCTCACGCCCCTGAGGACCCCTCGGATC

TGCTCCAGCACGTGAAATTCCAGTCCAGCAACTTTGAAAACATCCTGACGTGGGACAGCGGGCCGGAGGG

CACCCCAGACACGGTCTACAGCATCGAGTATAAGACGTACGGAGAGAGGGACTGGGTGGCAAAGAAGGGC

TGTCAGCGGATCACCCGGAAGTCCTGCAACCTGACGGTGGAGACGGGCAACCTCACGGAGCTCTACTATG

CCAGGGTCACCGCTGTCAGTGCGGGAGGCCGGTCAGCCACCAAGATGACTGACAGGTTCAGCTCTCTGCA

GCACACTACCCTCAAGCCACCTGATGTGACCTGTATCTCCAAAGTGAGATCGATTCAGATGATTGTTCAT

CCTACCCCCACGCCAATCCGTGCAGGCGATGGCCACCGGCTAACCCTGGAAGACATCTTCCATGACCTGT

TCTACCACTTAGAGCTCCAGGTCAACCGCACCTACCAAATGCACCTTGGAGGGAAGCAGAGAGAATATGA

GTTCTTCGGCCTGACCCCTGACACAGAGTTCCTTGGCACCATCATGATTTGCGTTCCCACCTGGGCCAAG

GAGAGTGCCCCCTACATGTGCCGAGTGAAGACACTGCCAGACCGGACATGGACCTACTCCTTCTCCGGAG

CCTTCCTGTTCTCCATGGGCTTCCTCGTCGCAGTACTCTGCTACCTGAGCTACAGATATGTCACCAAGCC

GCCTGCACCTCCCAACTCCCTGAACGTCCAGCGAGTCCTGACTTTCCAGCCGCTGCGCTTCATCCAGGAG

CACGTCCTGATCCCTGTCTTTGACCTCAGCGGCCCCAGCAGTCTGGCCCAGCCTGTCCAGTACTCCCAGA

TCAGGGTGTCTGGACCCAGGGAGCCCGCAGGAGCTCCACAGCGGCATAGCCTGTCCGAGATCACCTACTT

AGGGCAGCCAGACATCTCCATCCTCCAGCCCTCCAACGTGCCACCTCCCCAGATCCTCTCCCCACTGTCC

TATGCCCCAAACGCTGCCCCTGAGGTCGGGCCCCCATCCTATGCACCTCAGGTGACCCCCGAAGCTCAAT

TCCCATTCTACGCCCCACAGGCCATCTCTAAGGTCCAGCCTTCCTCCTATGCCCCTCAAGCCACTCCGGA

CAGCTGGCCTCCCTCCTATGGGGTATGCATGGAAGGTTCTGGCAAAGACTCCCCCACTGGGACACTTTCT

AGTCCTAAACACCTTAGGCCTAAAGGTCAGCTTCAGAAAGAGCCACCAGCTGGAAGCTGCATGTTAGGTG

GCCTTTCTCTGCAGGAGGTGACCTCCTTGGCTATGGAGGAATCCCAAGAAGCAAAATCATTGCACCAGCC

CCTGGGGATTTGCACAGACAGAACATCTGACCCAAATGTGCTACACAGTGGGGAGGAAGGGACACCACAG

TACCTAAAGGGCCAGCTCCCCCTCCTCTCCTCAGTCCAGATCGAGGGCCACCCCATGTCCCTCCCTTTGC

AACCTCCTTCCCGTCCATGTTCCCCCTCGGACCAAGGTCCAAGTCCCTGGGGCCTGCTGGAGTCCCTTGT

GTGTCCCAAGGATGAAGCCAAGAGCCCAGCCCCTGAGACCTCAGACCTGGAGCAGCCCACAGAACTGGAT

TCTCTTTTCAGAGGCCTGGCCCTGACTGTGCAGTGGGAGTCCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.