Human IFNAR1/IFNBR/CRF2-1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

IFNAR1 (interferon alpha/beta receptor subunit 1) is a single-pass type I transmembrane protein belonging to the type II cytokine receptor family. IFNAR1 is a component of the receptor complex for type I interferons, including interferons alpha, IFNB1 and IFNW1. IFNAR1 (alpha chain) functions in general as a heterodimer with IFNAR2 (beta chain). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT1/2 proteins and the IFNAR1 and IFNAR2 themselves. IFNAR1 can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. IFNAR1 also functions as an antiviral factor. 

 

 

Gene Symbol: IFNAR1; AVP; CRF2-1; IFRC; IFNAR; IFNBR; IFN-alpha-REC

 

NCBI Gene ID: 3454

 

Uniprot Entry: P17181

 

Construct Details: Full length human IFNAR1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1674 (bp)

 

Gene Insert Sequence:  

ATGATGGTCGTCCTCCTGGGCGCGACGACCCTAGTGCTCGTCGCCGTGGCGCCATGGGTGTTGTCCGCAG

CCGCAGGTGGAAAAAATCTAAAATCTCCTCAAAAAGTAGAGGTCGACATCATAGATGACAACTTTATCCT

GAGGTGGAACAGGAGCGATGAGTCTGTCGGGAATGTGACTTTTTCATTCGATTATCAAAAAACTGGGATG

GATAATTGGATAAAATTGTCTGGGTGTCAGAATATTACTAGTACCAAATGCAACTTTTCTTCACTCAAGC

TGAATGTTTATGAAGAAATTAAATTGCGTATAAGAGCAGAAAAAGAAAACACTTCTTCATGGTATGAGGT

TGACTCATTTACACCATTTCGCAAAGCTCAGATTGGTCCTCCAGAAGTACATTTAGAAGCTGAAGATAAG

GCAATAGTGATACACATCTCTCCTGGAACAAAAGATAGTGTTATGTGGGCTTTGGATGGTTTAAGCTTTA

CATATAGCTTAGTTATCTGGAAAAACTCTTCAGGTGTAGAAGAAAGGATTGAAAATATTTATTCCAGACA

TAAAATTTATAAACTCTCACCAGAGACTACTTATTGTCTAAAAGTTAAAGCAGCACTACTTACGTCATGG

AAAATTGGTGTCTATAGTCCAGTACATTGTATAAAGACCACAGTTGAAAATGAACTACCTCCACCAGAAA

ATATAGAAGTCAGTGTCCAAAATCAGAACTATGTTCTTAAATGGGATTATACATATGCAAACATGACCTT

TCAAGTTCAGTGGCTCCACGCCTTTTTAAAAAGGAATCCTGGAAACCATTTGTATAAATGGAAACAAATA

CCTGACTGTGAAAATGTCAAAACTACCCAGTGTGTCTTTCCTCAAAACGTTTTCCAAAAAGGAATTTACC

TTCTCCGCGTACAAGCATCTGATGGAAATAACACATCTTTTTGGTCTGAAGAGATAAAGTTTGATACTGA

AATACAAGCTTTCCTACTTCCTCCAGTCTTTAACATTAGATCCCTTAGTGATTCATTCCATATCTATATC

GGTGCTCCAAAACAGTCTGGAAACACGCCTGTGATCCAGGATTATCCACTGATTTATGAAATTATTTTTT

GGGAAAACACTTCAAATGCTGAGAGAAAAATTATCGAGAAAAAAACTGATGTTACAGTTCCTAATTTGAA

ACCACTGACTGTATATTGTGTGAAAGCCAGAGCACACACCATGGATGAAAAGCTGAATAAAAGCAGTGTT

TTTAGTGACGCTGTATGTGAGAAAACAAAACCAGGAAATACCTCTAAAATTTGGCTTATAGTTGGAATTT

GTATTGCATTATTTGCTCTCCCGTTTGTCATTTATGCTGCGAAAGTCTTCTTGAGATGCATCAATTATGT

CTTCTTTCCATCACTTAAACCTTCTTCCAGTATAGATGAGTATTTCTCTGAACAGCCATTGAAGAATCTT

CTGCTTTCAACTTCTGAGGAACAAATCGAAAAATGTTTCATAATTGAAAATATAAGCACAATTGCTACAG

TAGAAGAAACTAATCAAACTGATGAAGATCATAAAAAATACAGTTCCCAAACTAGCCAAGATTCAGGAAA

TTATTCTAATGAAGATGAAAGCGAAAGTAAAACAAGTGAAGAACTACAGCAGGACTTTGTATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.