Human IFNGR2/IFNGT1/AF1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

IFNGR2 (interferon gamma receptor 2) is a single-pass type I transmembrane protein belonging to the type II cytokine receptor family. IFNGR2 is the non-ligand-binding (beta) chain and heterodimerizes with IFNGR1, the ligand-binding chain (alpha) to form the IFN-gamma receptor complex, which activates the JAK/STAT signaling pathway after ligand-binding. IFNGR2 is required for signal transduction in contrast to other IFNGR1 subunit responsible for ligand binding. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as immunodeficiency 28 (IMD28) and familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. 

 

 

Gene Symbol: IFNGR2; AF1; IFGR2; IMD28; IFNGT1; AF-1

 

NCBI Gene ID: 3460

 

Uniprot Entry: P38484

 

Construct Details: Full length human IFNGR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1014 (bp)

 

Gene Insert Sequence:  

ATGCGACCGACGCTGCTGTGGTCGCTGCTGCTGCTGCTCGGAGTCTTCGCCGCCGCCGCCGCGGCCCCGC

CAGACCCTCTTTCCCAGCTGCCCGCTCCTCAGCACCCGAAGATTCGCCTGTACAACGCAGAGCAGGTCCT

GAGTTGGGAGCCAGTGGCCCTGAGCAATAGCACGAGGCCTGTTGTCTACCAAGTGCAGTTTAAATACACC

GACAGTAAATGGTTCACGGCCGACATCATGTCCATAGGGGTGAATTGTACACAGATCACAGCAACAGAGT

GTGACTTCACTGCCGCCAGTCCCTCAGCAGGCTTCCCAATGGATTTCAATGTCACTCTACGCCTTCGAGC

TGAGCTGGGAGCACTCCATTCTGCCTGGGTGACAATGCCTTGGTTTCAACACTATCGGAATGTGACTGTC

GGGCCTCCAGAAAACATTGAGGTGACCCCAGGAGAAGGCTCCCTCATCATCAGGTTCTCCTCTCCCTTTG

ACATCGCTGATACCTCCACGGCCTTTTTTTGTTATTATGTCCATTACTGGGAAAAAGGAGGAATCCAACA

GGTCAAAGGCCCTTTCAGAAGCAACTCCATTTCATTGGATAACTTAAAACCCTCCAGAGTGTACTGTTTA

CAAGTCCAGGCACAACTGCTTTGGAACAAAAGTAACATCTTTAGAGTCGGGCATTTAAGCAACATATCTT

GCTACGAAACAATGGCAGATGCCTCCACTGAGCTTCAGCAAGTCATCCTGATCTCCGTGGGAACATTTTC

GTTGCTGTCGGTGCTGGCAGGAGCCTGTTTCTTCCTGGTCCTGAAATATAGAGGCCTGATTAAATACTGG

TTTCACACTCCACCAAGCATCCCATTACAGATAGAAGAGTATTTAAAAGACCCAACTCAGCCCATCTTAG

AGGCCTTGGACAAGGACAGCTCACCAAAGGATGACGTCTGGGACTCTGTGTCCATTATCTCGTTTCCGGA

AAAGGAGCAAGAAGATGTTCTCCAAACGCTTTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.