Human ENPEP/CD249/APA/gp160 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ENPEP (glutamyl aminopeptidase), APA (aminopeptidase A) or CD249 is a single-pass type II membrane glycoprotein belonging to the peptidase M1 family.  ENPEP contains a 18-aa short N-terminal cytoplasmic tail, a transmembrane domain (TM) and a 918-aa extracellular domain (ECD) with 14 potential N-linked gylcosylation sites.  The large ECD contains a pentapeptide consensus sequence characteristic of members of the zinc ion-binding metalloproteinase superfamily.  ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It is also found in a variety of other tissues.  ENPEP may play a role in the catabolic pathway of the renin-angiotensin system and in the regulation of systemic arterial blood pressure. ENPEP may also plays a role in regulating glomerulus development and growth and differentiation of early B-lineage cells.

 

 

Gene Symbol: ENPEP; APA; CD249; gp160

 

NCBI Gene ID: 2028

 

Uniprot Entry: Q07075

 

Construct Details: Full length human ENPEP is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2874 (bp)

 

Gene Insert Sequence:  

ATGAACTTTGCGGAGAGAGAGGGCTCTAAGAGATACTGCATTCAAACGAAACATGTGGCCATTCTCTGTG

CGGTGGTGGTGGGTGTAGGATTAATAGTGGGACTTGCCGTGGGCTTGACCAGATCGTGTGACTCCAGCGG

GGACGGCGGGCCGGGCACTGCGCCAGCTCCTTCCCACCTGCCTTCTTCCACGGCCAGCCCCTCAGGTCCT

CCTGCCCAGGACCAGGACATCTGCCCGGCCAGTGAGGATGAGAGCGGACAGTGGAAAAACTTTCGACTGC

CGGACTTCGTCAACCCAGTCCACTACGACCTGCACGTGAAGCCCCTGTTGGAGGAGGACACCTACACGGG

CACCGTGAGCATCTCCATCAACCTGAGCGCTCCCACCCGGTACCTGTGGCTGCACCTCCGGGAGACCAGG

ATCACCCGGCTCCCGGAGCTGAAGAGGCCCTCTGGGGACCAGGTGCAAGTCCGGAGGTGTTTCGAGTACA

AAAAGCAGGAGTACGTGGTGGTCGAGGCGGAGGAAGAGCTTACCCCCAGCAGTGGAGATGGCCTGTATCT

CCTGACCATGGAGTTCGCCGGCTGGCTGAACGGCTCCCTCGTGGGATTTTATAGAACCACCTACACGGAG

AACGGACAAGTCAAGAGCATAGTGGCCACCGATCATGAACCAACAGATGCCAGGAAATCTTTTCCTTGTT

TTGATGAGCCCAACAAAAAGGCAACTTATACAATATCTATCACCCATCCCAAAGAATACGGAGCACTTTC

AAATATGCCAGTGGCGAAAGAAGAGTCAGTGGATGATAAATGGACTCGAACAACTTTTGAGAAGTCTGTC

CCCATGAGCACGTACCTGGTGTGCTTTGCTGTACATCAATTTGACTCTGTAAAGAGAATATCAAATAGTG

GAAAACCTCTTACAATTTATGTCCAGCCAGAGCAAAAGCACACAGCCGAATATGCTGCAAACATAACTAA

AAGTGTGTTTGATTATTTTGAAGAATACTTTGCTATGAATTATTCTCTTCCTAAATTAGATAAAATCGCT

ATTCCAGATTTTGGCACTGGTGCCATGGAGAACTGGGGACTCATCACGTACAGAGAAACGAACCTGCTTT

ATGACCCTAAGGAATCAGCCTCATCAAACCAACAGAGGGTGGCCACTGTGGTTGCCCATGAACTTGTGCA

TCAGTGGTTTGGAAATATTGTGACCATGGACTGGTGGGAAGACTTGTGGCTAAATGAAGGATTTGCTTCT

TTCTTTGAGTTTCTGGGAGTAAACCATGCAGAAACAGACTGGCAAATGCGTGACCAAATGTTACTTGAAG

ATGTATTACCTGTTCAAGAGGATGATTCTTTGATGTCTTCGCATCCAATTATTGTGACTGTGACAACCCC

TGATGAAATAACATCTGTTTTTGATGGAATATCCTATAGCAAGGGATCTTCTATTTTGAGAATGCTTGAA

GACTGGATAAAACCAGAGAATTTTCAAAAAGGATGTCAGATGTACTTGGAAAAATACCAATTCAAGAATG

CAAAAACTTCTGATTTTTGGGCAGCACTGGAAGAGGCAAGTAGGCTACCAGTGAAAGAAGTAATGGACAC

CTGGACCAGACAGATGGGTTATCCTGTGCTTAACGTGAACGGTGTCAAGAACATCACACAGAAACGCTTT

TTGTTGGACCCAAGAGCTAACCCTTCTCAGCCCCCTTCAGATCTTGGTTATACATGGAATATCCCAGTTA

AATGGACTGAAGATAATATAACAAGCAGTGTGTTATTTAATAGGTCAGAAAAAGAAGGAATCACTTTGAA

CTCCTCTAATCCTAGTGGAAATGCTTTTCTCAAAATAAACCCAGATCATATTGGGTTTTATCGTGTAAAT

TATGAAGTAGCAACTTGGGACTCGATAGCTACAGCGCTCTCCTTGAACCACAAGACATTTTCTTCAGCAG

ATCGTGCAAGTCTTATTGATGATGCTTTTGCCTTGGCAAGAGCTCAACTTCTAGATTATAAGGTGGCTTT

GAACTTGACCAAGTATCTCAAAAGGGAAGAGAATTTTTTACCATGGCAGAGAGTAATTTCAGCTGTAACC

TACATCATTAGCATGTTTGAAGATGATAAAGAGCTATATCCTATGATTGAGGAATACTTCCAAGGTCAAG

TGAAGCCTATTGCAGATTCTCTGGGATGGAATGATGCTGGAGACCATGTCACAAAGTTACTCCGTTCCTC

CGTGTTAGGGTTTGCGTGCAAGATGGGAGACAGAGAAGCCTTGAACAATGCTTCCTCGTTATTTGAGCAG

TGGCTAAATGGGACTGTAAGCCTTCCCGTAAATCTCAGGCTTCTGGTGTATCGGTATGGGATGCAGAACT

CTGGCAATGAGATTTCATGGAACTACACTCTTGAGCAATACCAGAAAACTTCATTAGCTCAAGAAAAAGA

AAAACTGCTGTATGGATTAGCATCAGTGAAGAACGTTACTCTTTTGTCAAGGTATTTGGATTTGCTCAAG

GACACGAACCTTATTAAAACTCAGGATGTGTTTACAGTCATTCGATATATCTCATATAACAGCTATGGGA

AGAACATGGCCTGGAATTGGATACAACTCAACTGGGACTATCTAGTCAACAGATATACACTCAATAACAG

AAACCTTGGCCGAATTGTCACAATAGCAGAGCCATTCAACACTGAACTGCAACTGTGGCAGATGGAGAGC

TTTTTTGCAAAATATCCACAAGCTGGAGCAGGAGAAAAACCTAGGGAACAAGTGCTGGAAACAGTGAAAA

ACAATATAGAGTGGCTAAAACAACATAGAAACACCATCAGAGAATGGTTTTTTAATTTACTTGAGAGTGG

TTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.