Human PTGDR2/CD294/DP2/CRTH2 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

PTGDR2 (prostaglandin D2 receptor 2), CRTH2 (chemoattractant receptor-homologous molecule expressed on TH2 cells) or CD294,  is a 7-transmembrane protein of the G protein-coupled receptor 1 (GPCR1) family.  PTGDR2 is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. PTGDR2 is a prostaglandin D2 (PGD2) receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. PTGDR2 signaling is coupled to the G(i)-protein. Its activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induces PTGDR2 internalization. that can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. PTGDR2 signaling activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.

 

 

Gene Symbol: PTGDR2; CD294; DP2; DL1R; CRTH2; GPR44

 

NCBI Gene ID: 11251

 

Uniprot Entry: Q9Y5Y4

 

Construct Details: Full length human PTGDR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1188 (bp)

 

Gene Insert Sequence:  

ATGTCGGCCAACGCCACACTGAAGCCACTCTGCCCCATCCTGGAGCAGATGAGCCGTCTCCAGAGCCACA

GCAACACCAGCATCCGCTACATCGACCACGCGGCCGTGCTGCTGCACGGGCTGGCCTCGCTGCTGGGCCT

GGTGGAGAATGGAGTCATCCTCTTCGTGGTGGGCTGCCGCATGCGCCAGACCGTGGTCACCACCTGGGTG

CTGCACCTGGCGCTGTCCGACCTGTTGGCCTCTGCTTCCCTGCCCTTCTTCACCTACTTCTTGGCCGTGG

GCCACTCGTGGGAGCTGGGCACCACCTTCTGCAAACTGCACTCCTCCATCTTCTTTCTCAACATGTTCGC

CAGCGGCTTCCTGCTCAGCGCCATCAGCCTGGACCGCTGCCTGCAGGTGGTGCGGCCGGTGTGGGCGCAG

AACCACCGCACCGTGGCCGCGGCGCACAAAGTCTGCCTGGTGCTTTGGGCACTAGCGGTGCTCAACACGG

TGCCCTATTTCGTGTTCCGGGACACCATCTCGCGGCTGGACGGGCGCATTATGTGCTACTACAATGTGCT

GCTCCTGAACCCGGGGCCTGACCGCGATGCCACGTGCAACTCGCGGCAGGTGGCCCTGGCCGTCAGCAAG

TTCCTGCTGGCCTTCCTGGTGCCGCTGGCGATCATCGCCTCGAGCCACGCGGCCGTGAGCCTGCGGTTGC

AGCACCGCGGCCGCCGGCGGCCAGGCCGCTTCGTGCGCCTGGTGGCGGCCGTCGTGGCCGCCTTCGCGCT

CTGCTGGGGGCCCTACCACGTGTTCAGCCTGCTGGAGGCGCGGGCGCACGCAAACCCGGGGCTGCGGCCG

CTCGTGTGGCGCGGGCTGCCCTTCGTCACCAGCCTGGCCTTCTTCAACAGCGTGGCCAACCCGGTGCTCT

ACGTGCTCACCTGCCCCGACATGCTGCGCAAGCTGCGGCGCTCGCTGCGCACGGTGCTGGAGAGCGTGCT

GGTGGACGACAGCGAGCTGGGTGGCGCGGGAAGCAGCCGCCGCCGCCGCACCTCCTCCACCGCCCGCTCG

GCCTCCCCTTTAGCTCTCTGCAGCCGCCCGGAGGAACCGCGGGGCCCCGCGCGTCTCCTCGGCTGGCTGC

TGGGCAGCTGCGCAGCGTCCCCGCAGACGGGCCCCCTGAACCGGGCGCTGAGCAGCACCTCGAGTTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.