Human ATP4B Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ATP4B (ATPase H+/K+ transporting beta subunit) is a single-pass type II transmembrane glycoprotein beloning to the family of Na+/K+ and H+/K+ ATPases beta chain proteins. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This ATPase is composed of three subunits: a large catalytic subunit alpha (catalytic), a smaller glycoprotein subunit beta and gamma. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. ATP4B is the beta subunit of the gastric H+, K+-ATPase.  The gastric H+, K+-ATPase is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.  ATP4B may be required for stabilization and maturation of the catalytic proton pump alpha subunit.  The C-terminal lobe of ATP4B folds into an immunoglobulin-like domain and may mediate cell adhesion and establish epithelial cell polarity.

 

 

Gene Symbol: ATP4B

 

NCBI Gene ID: 496

 

Uniprot Entry: P51164

 

Construct Details: Full length human ATP4B is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 876 (bp)

 

Gene Insert Sequence:  

ATGGCGGCTCTGCAGGAGAAGAAGACGTGTGGCCAGCGCATGGAGGAGTTCCAGCGTTACTGCTGGAACC

CGGACACGGGGCAGATGCTGGGCCGCACCCTGTCCCGGTGGGTGTGGATCAGCCTGTACTACGTGGCCTT

CTACGTGGTGATGACTGGGCTCTTCGCCCTGTGCCTCTATGTGCTGATGCAGACAGTGGACCCGTACACA

CCGGACTACCAAGACCAGCTACGGTCACCAGGGGTAACCTTAAGGCCGGATGTTTACGGGGAGAAAGGCC

TGGAAATTGTCTACAACGTCTCTGATAACAGAACCTGGGCAGACCTCACACAGACTCTCCACGCCTTCCT

AGCAGGCTACTCTCCAGCAGCCCAGGAGGACAGCATCAACTGCACCTCCGAGCAGTACTTCTTCCAGGAG

AGTTTCCGCGCTCCCAACCACACCAAGTTCTCCTGCAAGTTCACGGCAGATATGCTGCAGAACTGCTCAG

GCCTGGCGGATCCCAACTTCGGCTTTGAAGAAGGAAAGCCATGTTTTATTATTAAAATGAACAGGATCGT

CAAGTTCCTCCCCAGCAACGGCTCGGCCCCCAGAGTGGACTGCGCCTTCCTGGACCAGCCCCGCGAGCTC

GGCCAGCCGCTGCAGGTCAAGTACTACCCTCCCAACGGCACCTTCAGTCTGCACTACTTCCCTTATTACG

GGAAGAAAGCCCAGCCCCACTACAGCAACCCCCTGGTGGCAGCGAAGCTCCTCAACATCCCCAGGAACGC

TGAGGTCGCCATCGTGTGCAAGGTCATGGCGGAGCACGTGACCTTCAACAATCCCCACGACCCGTATGAA

GGGAAAGTGGAGTTCAAACTCAAGATTGAGAAGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.