Human EMG/EMBIGIN/GP70 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

EMB or Embigin is a 327 amino acid (aa) single pass type I transmembrane protein of the Basigin family of the Immunoglobulin superfamily (IgSF).  BEMB contains an N-terminal signal peptide, a 228-aa extracellular domain (ECD) with 2 Ig-like V-type domains and 9 potential N-linked glycosylation sites, a transmembrane domain (TM), and a 46-aa cytoplasmic tail.  EMB may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. EMB plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. EMB  may play a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane.

 

 

Gene Symbol: EMB; Embigin; GP70

 

NCBI Gene ID: 133418

 

Uniprot Entry: Q6PCB8

 

Construct Details: Full length human EMB gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotics selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 984 bp

 

Gene Insert Sequence:  

ATGCGCGCCCTCCCCGGCCTGCTGGAGGCCAGGGCGCGTACGCCCCGGCTGCTCCTCCTCCAGTGCCTTC

TCGCTGCCGCGCGCCCAAGCTCGGCGGACGGCAGTGCCCCAGATTCGCCTTTTACAAGTCCACCTCTCAG

AGAAGAAATAATGGCAAATAACTTTTCCTTGGAGAGTCATAACATATCACTGACTGAACATTCTAGTATG

CCAGTAGAAAAAAATATCACTTTAGAAAGGCCTTCTAATGTAAATCTCACATGCCAGTTCACAACATCTG

GGGATTTGAATGCAGTAAATGTGACTTGGAAAAAAGATGGTGAACAACTTGAGAATAATTATCTTGTCAG

TGCAACAGGAAGCACCTTGTATACCCAATACAGGTTCACCATCATTAATAGCAAACAAATGGGAAGTTAT

TCTTGTTTCTTTCGAGAGGAAAAGGAACAAAGGGGAACATTTAATTTCAAAGTCCCTGAACTTCATGGGA

AAAACAAGCCATTGATCTCTTACGTAGGGGATTCTACTGTCTTGACATGTAAATGTCAAAATTGTTTTCC

TTTAAATTGGACCTGGTACAGTAGTAATGGGAGTGTAAAGGTTCCTGTTGGTGTTCAAATGAATAAATAT

GTGATCAATGGAACATATGCTAACGAAACAAAGCTGAAGATAACACAACTTTTGGAGGAAGATGGGGAAT

CTTACTGGTGCCGTGCACTATTCCAATTAGGCGAGAGTGAAGAACACATTGAGCTTGTGGTGCTGAGCTA

TTTGGTGCCCCTCAAACCATTTCTTGTAATAGTGGCTGAGGTGATTCTTTTAGTGGCCACCATTCTGCTT

TGTGAAAAGTACACACAAAAGAAAAAGAAGCACTCAGATGAGGGGAAAGAATTTGAGCAGATTGAACAGC

TGAAATCAGATGATAGCAATGGTATAGAAAATAATGTCCCCAGGCATAGAAAAAATGAGTCTCTGGGCCA

GTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.