Human JAG2/hJ2/SER2/Jagged2 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

JAG2 (Jagged2) is a 1238 amino acid (aa) single pass type I transmembrane glycoprotein that is the human homolog of the Drosophilia jagged protein.  JAG2 contains an N-terminal signal peptide, a 1054-aa extracellular domain (ECD) with a DSL domain and 16 EGF-like domains, a transmembrane domain (TM), and a 137-aa cytoplasmic tail.   JAG2 is involved in the mediation of Notch signaling.  Human JAG2 is a putative ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor NOTCH.  The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development and cell fate decisions. JAG2 is one of several ligands that activate Notch and related receptors.  JAG2 may be involved in limb development.  

 

 

Gene Symbol: JAG2; hJ2; SER2; Jagged2

 

NCBI Gene ID: 3714

 

Uniprot Entry: Q9Y219

 

Construct Details: Full length human JAG2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3717 (bp)

 

Gene Insert Sequence:  

ATGCGGGCGCAGGGCCGGGGGCGCCTTCCCCGGCGGCTGCTGCTGCTGCTGGCGCTCTGGGTGCAGGCGG

CGCGGCCCATGGGCTATTTCGAGCTGCAGCTGAGCGCGCTGCGGAACGTGAACGGGGAGCTGCTGAGCGG

CGCCTGCTGTGACGGCGACGGCCGGACAACGCGCGCGGGGGGCTGCGGCCACGACGAGTGCGACACGTAC

GTGCGCGTGTGCCTTAAGGAGTACCAGGCCAAGGTGACGCCCACGGGGCCCTGCAGCTACGGCCACGGCG

CCACGCCCGTGCTGGGCGGCAACTCCTTCTACCTGCCGCCGGCGGGCGCTGCGGGGGACCGAGCGCGGGC

GCGGGCCCGGGCCGGCGGCGACCAGGACCCGGGCCTCGTCGTCATCCCCTTCCAGTTCGCCTGGCCGCGC

TCCTTTACCCTCATCGTGGAGGCCTGGGACTGGGACAACGATACCACCCCGAATGAGGAGCTGCTGATCG

AGCGAGTGTCGCATGCCGGCATGATCAACCCGGAGGACCGCTGGAAGAGCCTGCACTTCAGCGGCCACGT

GGCGCACCTGGAGCTGCAGATCCGCGTGCGCTGCGACGAGAACTACTACAGCGCCACTTGCAACAAGTTC

TGCCGGCCCCGCAACGACTTTTTCGGCCACTACACCTGCGACCAGTACGGCAACAAGGCCTGCATGGACG

GCTGGATGGGCAAGGAGTGCAAGGAAGCTGTGTGTAAACAAGGGTGTAATTTGCTCCACGGGGGATGCAC

CGTGCCTGGGGAGTGCAGGTGCAGCTACGGCTGGCAAGGGAGGTTCTGCGATGAGTGTGTCCCCTACCCC

GGCTGCGTGCATGGCAGTTGTGTGGAGCCCTGGCAGTGCAACTGTGAGACCAACTGGGGCGGCCTGCTCT

GTGACAAAGACCTGAACTACTGTGGCAGCCACCACCCCTGCACCAACGGAGGCACGTGCATCAACGCCGA

GCCTGACCAGTACCGCTGCACCTGCCCTGACGGCTACTCGGGCAGGAACTGTGAGAAGGCTGAGCACGCC

TGCACCTCCAACCCGTGTGCCAACGGGGGCTCTTGCCATGAGGTGCCGTCCGGCTTCGAATGCCACTGCC

CATCGGGCTGGAGCGGGCCCACCTGTGCCCTTGACATCGATGAGTGTGCTTCGAACCCGTGTGCGGCCGG

TGGCACCTGTGTGGACCAGGTGGACGGCTTTGAGTGCATCTGCCCCGAGCAGTGGGTGGGGGCCACCTGC

CAGCTGGACGCCAATGAGTGTGAAGGGAAGCCATGCCTTAACGCTTTTTCTTGCAAAAACCTGATTGGCG

GCTATTACTGTGATTGCATCCCGGGCTGGAAGGGCATCAACTGCCATATCAACGTCAACGACTGTCGCGG

GCAGTGTCAGCATGGGGGCACCTGCAAGGACCTGGTGAACGGGTACCAGTGTGTGTGCCCACGGGGCTTC

GGAGGCCGGCATTGCGAGCTGGAACGAGACGAGTGTGCCAGCAGCCCCTGCCACAGCGGCGGCCTCTGCG

AGGACCTGGCCGACGGCTTCCACTGCCACTGCCCCCAGGGCTTCTCCGGGCCTCTCTGTGAGGTGGATGT

CGACCTTTGTGAGCCAAGCCCCTGCCGGAACGGCGCTCGCTGCTATAACCTGGAGGGTGACTATTACTGC

GCCTGCCCTGATGACTTTGGTGGCAAGAACTGCTCCGTGCCCCGCGAGCCGTGCCCTGGCGGGGCCTGCA

GAGTGATCGATGGCTGCGGGTCAGACGCGGGGCCTGGGATGCCTGGCACAGCAGCCTCCGGCGTGTGTGG

CCCCCATGGACGCTGCGTCAGCCAGCCAGGGGGCAACTTTTCCTGCATCTGTGACAGTGGCTTTACTGGC

ACCTACTGCCATGAGAACATTGACGACTGCCTGGGCCAGCCCTGCCGCAATGGGGGCACATGCATCGATG

AGGTGGACGCCTTCCGCTGCTTCTGCCCCAGCGGCTGGGAGGGCGAGCTCTGCGACACCAATCCCAACGA

CTGCCTTCCCGATCCCTGCCACAGCCGCGGCCGCTGCTACGACCTGGTCAATGACTTCTACTGTGCGTGC

GACGACGGCTGGAAGGGCAAGACCTGCCACTCACGCGAGTTCCAGTGCGATGCCTACACCTGCAGCAACG

GTGGCACCTGCTACGACAGCGGCGACACCTTCCGCTGCGCCTGCCCCCCCGGCTGGAAGGGCAGCACCTG

CGCCGTCGCCAAGAACAGCAGCTGCCTGCCCAACCCCTGTGTGAATGGTGGCACCTGCGTGGGCAGCGGG

GCCTCCTTCTCCTGCATCTGCCGGGACGGCTGGGAGGGTCGTACTTGCACTCACAATACCAACGACTGCA

ACCCTCTGCCTTGCTACAATGGTGGCATCTGTGTTGACGGCGTCAACTGGTTCCGCTGCGAGTGTGCACC

TGGCTTCGCGGGGCCTGACTGCCGCATCAACATCGACGAGTGCCAGTCCTCGCCCTGTGCCTACGGGGCC

ACGTGTGTGGATGAGATCAACGGGTATCGCTGTAGCTGCCCACCCGGCCGAGCCGGCCCCCGGTGCCAGG

AAGTGATCGGGTTCGGGAGATCCTGCTGGTCCCGGGGCACTCCGTTCCCACACGGAAGCTCCTGGGTGGA

AGACTGCAACAGCTGCCGCTGCCTGGATGGCCGCCGTGACTGCAGCAAGGTGTGGTGCGGATGGAAGCCT

TGTCTGCTGGCCGGCCAGCCCGAGGCCCTGAGCGCCCAGTGCCCACTGGGGCAAAGGTGCCTGGAGAAGG

CCCCAGGCCAGTGTCTGCGACCACCCTGTGAGGCCTGGGGGGAGTGCGGCGCAGAAGAGCCACCGAGCAC

CCCCTGCCTGCCACGCTCCGGCCACCTGGACAATAACTGTGCCCGCCTCACCTTGCATTTCAACCGTGAC

CACGTGCCCCAGGGCACCACGGTGGGCGCCATTTGCTCCGGGATCCGCTCCCTGCCAGCCACAAGGGCTG

TGGCACGGGACCGCCTGCTGGTGTTGCTTTGCGACCGGGCGTCCTCGGGGGCCAGTGCTGTGGAGGTGGC

CGTGTCCTTCAGCCCTGCCAGGGACCTGCCTGACAGCAGCCTGATCCAGGGCGCGGCCCACGCCATCGTG

GCCGCCATCACCCAGCGGGGGAACAGCTCACTGCTCCTGGCTGTCACCGAGGTCAAGGTGGAGACGGTTG

TTACGGGCGGCTCTTCCACAGGTCTGCTGGTGCCTGTGCTGTGTGGTGCCTTCAGCGTGCTGTGGCTGGC

GTGCGTGGTCCTGTGCGTGTGGTGGACACGCAAGCGCAGGAAAGAGCGGGAGAGGAGCCGGCTGCCGCGG

GAGGAGAGCGCCAACAACCAGTGGGCCCCGCTCAACCCCATCCGCAACCCCATTGAGCGGCCGGGGGGCC

ACAAGGACGTGCTCTACCAGTGCAAGAACTTCACGCCGCCGCCGCGCAGGGCGGACGAGGCGCTGCCCGG

GCCGGCCGGCCACGCGGCCGTCAGGGAGGATGAGGAGGACGAGGATCTGGGCCGCGGTGAGGAGGACTCC

CTGGAGGCGGAGAAGTTCCTCTCACACAAATTCACCAAAGATCCTGGCCGCTCGCCGGGGAGGCCGGCCC

ACTGGGCCTCAGGCCCCAAAGTGGACAACCGCGCGGTCAGGAGCATCAATGAGGCCCGCTACGCCGGCAA

GGAGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Additional supporting documents, including product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.