Human ABCG8/GBD4 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ABCG8 (ATP-binding cassette sub-family G member 8) is a six-pass type II transmembrane glycoprotein beloning to the ABC transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White/ABCG). ABCG8 is a member of the White/ABCG subfamily. ABCG1 plays an important role in cellular cholesterol homeostasis. ABCG8  appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. ABCG8 functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. ABCG8 is expressed in a tissue-specific manner in the liver, intestine, and gallbladder.  ABCG8 may form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion. 

 

 

Gene Symbol: ABCG8; GBD4; STSL

 

NCBI Gene ID: 64241

 

Uniprot Entry: Q9H221

 

Construct Details: Full length human ABCG8 is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2022 (bp)

 

Gene Insert Sequence:  

ATGGCCGGGAAGGCGGCAGAGGAGAGAGGGCTGCCGAAAGGGGCCACTCCCCAGGATACCTCGGGCCTCC

AGGATAGATTGTTCTCCTCTGAAAGTGACAACAGCCTGTACTTCACCTACAGTGGCCAGCCCAACACCCT

GGAGGTCAGAGACCTCAACTACCAGGTGGACCTGGCCTCTCAGGTCCCTTGGTTTGAGCAGCTGGCTCAG

TTCAAGATGCCCTGGACATCTCCCAGCTGCCAGAATTCTTGTGAGCTGGGCATCCAGAACCTAAGCTTCA

AAGTGAGAAGTGGGCAGATGCTGGCCATCATAGGGAGCTCAGGTTGTGGGAGAGCCTCCTTGCTAGATGT

GATCACTGGCCGAGGTCACGGCGGCAAGATCAAGTCAGGCCAGATCTGGATCAATGGGCAGCCCAGCTCG

CCTCAGCTGGTGAGGAAGTGTGTGGCCCACGTGCGCCAGCACAACCAGCTGCTCCCCAACTTGACTGTGC

GAGAGACCTTGGCCTTCATTGCCCAGATGCGGCTGCCCAGAACCTTCTCCCAGGCCCAGCGTGACAAAAG

GGTGGAGGACGTGATCGCGGAGCTGCGGCTTAGGCAGTGCGCTGACACCCGCGTGGGCAACATGTACGTG

CGGGGGTTGTCGGGGGGTGAGCGCAGGAGAGTCAGCATTGGGGTGCAGCTCCTGTGGAACCCAGGAATCC

TTATTCTCGACGAACCCACCTCTGGGCTCGACAGCTTCACAGCCCACAACCTGGTGAAGACCTTGTCCAG

GCTGGCCAAAGGCAACCGGCTGGTGCTCATCTCCCTCCACCAGCCTCGCTCTGACATCTTCAGGCTGTTT

GATCTGGTCCTCCTGATGACGTCTGGCACCCCCATCTACTTAGGGGCGGCCCAGCACATGGTCCAGTATT

TCACAGCCATCGGCTACCCCTGTCCTCGCTACAGCAATCCTGCTGACTTCTATGTGGACCTGACCAGCAT

TGACAGGCGCAGCAGAGAGCAGGAATTGGCCACCAGGGAGAAGGCTCAGTCACTCGCAGCCCTGTTTCTA

GAAAAAGTGCGTGACTTAGATGACTTTCTATGGAAAGCAGAGACGAAGGATCTTGACGAGGACACCTGTG

TGGAAAGCAGCGTGACCCCACTAGACACCAACTGCCTCCCGAGTCCTACGAAGATGCCTGGGGCGGTGCA

GCAGTTTACGACGCTGATCCGTCGTCAGATTTCCAACGACTTCCGAGACCTGCCCACCCTCCTCATCCAT

GGGGCGGAGGCCTGTCTGATGTCAATGACCATCGGCTTCCTCTATTTTGGCCATGGGAGCATCCAGCTCT

CCTTCATGGATACAGCCGCCCTCTTGTTCATGATCGGTGCTCTCATCCCTTTCAACGTCATTCTGGATGT

CATCTCCAAATGTTACTCAGAGAGGGCAATGCTTTACTATGAACTGGAAGACGGGCTGTACACCACTGGT

CCATATTTCTTTGCCAAGATCCTCGGGGAGCTTCCGGAGCACTGTGCCTACATCATCATCTACGGGATGC

CCACCTACTGGCTGGCCAACCTGAGGCCAGGCCTCCAGCCCTTCCTGCTGCACTTCCTGCTGGTGTGGCT

GGTGGTCTTCTGTTGCAGGATTATGGCCCTGGCCGCCGCGGCCCTGCTCCCCACCTTCCACATGGCCTCC

TTCTTCAGCAATGCCCTCTACAACTCCTTCTACCTCGCCGGGGGCTTCATGATAAACTTGAGCAGCCTGT

GGACAGTGCCCGCGTGGATTTCCAAAGTGTCCTTCCTGCGGTGGTGTTTTGAAGGGCTGATGAAGATTCA

GTTCAGCAGAAGAACTTATAAAATGCCTCTCGGGAACCTCACCATCGCGGTCTCAGGAGATAAAATCCTC

AGTGTCATGGAGCTGGACTCGTACCCTCTCTACGCCATCTACCTCATCGTCATTGGCCTCAGCGGTGGCT

TCATGGTCCTGTACTACGTGTCCTTAAGGTTCATCAAACAGAAACCAAGTCAAGACTGGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.