Human ABCG5/STSL Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ABCG5 (ATP-binding cassette sub-family G member 5) is a six-pass type II transmembrane glycoprotein beloning to the ABC transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White/ABCG). ABCG4 is a member of the White/ABCG subfamily. ABCG1 plays an important role in cellular cholesterol homeostasis. ABCG5 functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in the ABCG5 gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia (STSL), an autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. ABCG5 may form heterodimers with ABCG8 or be tightly coupled to ABCG8 along a pathway regulating diatery-sterol absorption and excretion.

 

 

Gene Symbol: ABCG5; STSL

 

NCBI Gene ID: 64240

 

Uniprot Entry: Q9H222

 

Construct Details: Full length human ABCG5 is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1956 (bp)

 

Gene Insert Sequence:  

ATGGGTGACCTCTCATCTTTGACCCCCGGAGGGTCCATGGGTCTCCAAGTAAACAGAGGCTCCCAGAGCT

CCCTGGAGGGGGCTCCTGCCACCGCCCCGGAGCCTCACAGCCTGGGCATCCTCCATGCCTCCTACAGCGT

CAGCCACCGCGTGAGGCCCTGGTGGGACATCACATCTTGCCGGCAGCAGTGGACCAGGCAGATCCTCAAA

GATGTCTCCTTGTACGTGGAGAGCGGGCAGATCATGTGCATCCTAGGAAGCTCAGGCTCCGGGAAAACCA

CGCTGCTGGACGCCATGTCCGGGAGGCTGGGGCGCGCGGGGACCTTCCTGGGGGAGGTGTATGTGAACGG

CCGGGCGCTGCGCCGGGAGCAGTTCCAGGACTGCTTCTCCTACGTCCTGCAGAGCGACACCCTGCTGAGC

AGCCTCACCGTGCGCGAGACGCTGCACTACACCGCGCTGCTGGCCATCCGCCGCGGCAATCCCGGCTCCT

TCCAGAAGAAGGTGGAGGCCGTCATGGCAGAGCTGAGTCTGAGCCATGTGGCAGACCGACTGATTGGCAA

CTACAGCTTGGGGGGCATTTCCACGGGTGAGCGGCGCCGGGTCTCCATCGCAGCCCAGCTGCTCCAGGAT

CCTAAGGTCATGCTGTTTGATGAGCCAACCACAGGCCTGGACTGCATGACTGCTAATCAGATTGTCGTCC

TCCTGGTGGAACTGGCTCGCAGGAACCGAATTGTGGTTCTCACCATTCACCAGCCCCGTTCTGAGCTTTT

TCAGCTCTTTGACAAAATTGCCATCCTGAGCTTCGGAGAGCTGATTTTCTGTGGCACGCCAGCGGAAATG

CTTGATTTCTTCAATGACTGCGGTTACCCTTGTCCTGAACATTCAAACCCTTTTGACTTCTATATGGACC

TGACGTCAGTGGATACCCAAAGCAAGGAACGGGAAATAGAAACCTCCAAGAGAGTCCAGATGATAGAATC

TGCCTACAAGAAATCAGCAATTTGTCATAAAACTTTGAAGAATATTGAAAGAATGAAACACCTGAAAACG

TTACCAATGGTTCCTTTCAAAACCAAAGATTCTCCTGGAGTTTTCTCTAAACTGGGTGTTCTCCTGAGGA

GAGTGACAAGAAACTTGGTGAGAAATAAGCTGGCAGTGATTACGCGTCTCCTTCAGAATCTGATCATGGG

TTTGTTCCTCCTTTTCTTCGTTCTGCGGGTCCGAAGCAATGTGCTAAAGGGTGCTATCCAGGACCGCGTA

GGTCTCCTTTACCAGTTTGTGGGCGCCACCCCGTACACAGGCATGCTGAACGCTGTGAATCTGTTTCCCG

TGCTGCGAGCTGTCAGCGACCAGGAGAGTCAGGACGGCCTCTACCAGAAGTGGCAGATGATGCTGGCCTA

TGCACTGCACGTCCTCCCCTTCAGCGTTGTTGCCACCATGATTTTCAGCAGTGTGTGCTACTGGACGCTG

GGCTTACATCCTGAGGTTGCCCGATTTGGATATTTTTCTGCTGCTCTCTTGGCCCCCCACTTAATTGGTG

AATTTCTAACTCTTGTGCTACTTGGTATCGTCCAAAATCCAAATATAGTCAACAGTGTAGTGGCTCTGCT

GTCCATTGCGGGGGTGCTTGTTGGATCTGGATTCCTCAGAAACATACAAGAAATGCCCATTCCTTTTAAA

ATCATCAGTTATTTTACATTCCAAAAATATTGCAGTGAGATTCTTGTAGTCAATGAGTTCTACGGACTGA

ATTTCACTTGTGGCAGCTCAAATGTTTCTGTGACAACTAATCCAATGTGTGCCTTCACTCAAGGAATTCA

ATTCATTGAGAAAACCTGCCCAGGTGCAACATCTAGATTCACAATGAACTTTCTGATTTTGTATTCATTT

ATTCCAGCTCTTGTCATCCTAGGAATAGTTGTTTTCAAAATAAGGGATCATCTCATTAGCAGGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.