Human JAM4/IgSF5 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

JAM4 (Junctional adhesion molecule 4) or IgSF5 is a 407 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the Immunoglobulin superfamily (IgSF).  JAM4 contains an N-terminal signal peptide, a 266-aa extracellular domain (ECD) with 2 Ig-like V-type domains and 4 potential glycosylation sites, a transmembrane domain (TM), and a 122-aa cytoplasmic tail. JAM4 provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. JAM4 mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. JAM4may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells.

 

 

Gene Symbol: JAM4; IgSF5; GSF5; JAM-4

 

NCBI Gene ID: 150084

 

Uniprot Entry: Q9NSI5

 

Construct Details: Full length human JAM4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1224 (bp)

 

Gene Insert Sequence:  

ATGGGTCAGAAGGAAAGGAGCACAGCAGATACTCTGCCGGATCTGGAGGAATGGAAGTCAGCGGCGGGTC

TGCGATGGTGGCAAACAGCAGTGGTGGACGGTTCTGGGTCTGGTAATGAAGTCATAGAAGGCCCCCAAAA

TGCAAGAGTCCTGAAGGGCTCCCAGGCTCGCTTCAACTGCACCGTCTCCCAGGGCTGGAAGCTCATCATG

TGGGCTCTCAGTGACATGGTGGTGCTAAGCGTCAGGCCCATGGAGCCCATCATCACCAATGACCGCTTCA

CCTCTCAGAGGTACGACCAGGGCGGGAACTTCACCTCGGAGATGATCATCCACAATGTGGAGCCCAGTGA

TTCGGGGAACATCAGATGCAGCCTCCAGAACAGTCGCCTGCATGGATCTGCTTACCTTACCGTCCAAGTT

ATGGGAGAGCTGTTCATTCCCAGTGTTAATCTTGTAGTCGCTGAGAATGAACCTTGTGAAGTTACTTGTC

TACCCTCACACTGGACCCGGCTCCCGGATATTTCCTGGGAGCTCGGTCTCCTGGTCAGCCATTCAAGCTA

TTATTTTGTTCCGGAGCCCAGCGACCTTCAAAGTGCAGTGAGCATCCTGGCTCTGACCCCACAGAGCAAT

GGGACTTTGACTTGCGTGGCTACCTGGAAGAGCCTGAAGGCCCGCAAGTCTGCAACTGTAAATCTCACTG

TGATTCGGTGTCCCCAAGACACTGGAGGTGGTATTAATATTCCAGGTGTATTATCAAGTTTACCGAGTTT

AGGTTTTTCATTGCCTACTTGGGGCAAAGTTGGACTTGGACTAGCAGGCACCATGCTTCTGACGCCGACG

TGTACTCTTACAATACGCTGCTGCTGCTGCCGCCGTCGTTGTTGTGGCTGCAACTGCTGCTGCCGTTGTT

GTTTCTGCTGTAGAAGAAAAAGAGGATTTCGTATTCAATTTCAAAAGAAATCTGAAAAAGAGAAGACAAA

CAAAGAAACTGAGACAGAAAGTGGAAATGAAAACTCCGGCTACAATTCAGATGAACAAAAGACCACAGAC

ACCGCTTCTCTCCCTCCCAAATCCTGTGAATCCAGTGATCCTGAACAAAGAAACAGTAGCTGTGGCCCTC

CTCACCAGCGGGCTGATCAACGTCCACCCAGGCCAGCAAGTCATCCACAGGCTTCTTTTAATCTGGCCAG

TCCTGAGAAGGTCAGTAATACAACTGTAGTATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.