Human MAG/GMA/SIGLEC4A/SPG75 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

MAG (myelin associated glycoprotein) or SIGLEC4A is a single-pass type I transmembrane protein belonging to the sialic acid binding Ig-like lectin (SIGLEC) family in the immunoglobulin superfamily (IgSF). SIGLECs are characterized by an N-terminal Ig-like V-type (IgV)domain that mediates sialic acid binding, followed by varying numbers (2 to 17) of Ig-like C2-type (IgC2) domains in the extracellular region, a transmembrane region, and a cytoplasmic tail with intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM). They can be classified into two subgroups: Siglecs-1, -2, and -4 subgroup, and a Siglec-3/CD33-related subgroup (Siglecs-3, and -5 through -13), defined by sequence similarity and clustered gene localization. MAG contains 4 IgC2 domains and binds to sialylated gangliosides to mediate certain myelin-neuron cell-cell interactions.  MAG peferentially binds to alpha-2,3-linked sialic acid and ganglioside Gt1b.  MAG protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. MAG inhibits axon outgrowth by binding to RTN4R.  MAG is required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane.  Mutations affecting the MAG gene are associated with Spastic paraplegia 75 (SPG75),  an autosomal recessive, neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs..

 

 

Gene Symbol: MAG; GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A

 

NCBI Gene ID: 4099

 

Uniprot Entry: P20916

 

Construct Details: Full length human MAG gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotics selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1881 bp

 

Gene Insert Sequence:  

ATGATATTCCTCACGGCACTGCCTCTGTTCTGGATTATGATTTCAGCCTCCCGAGGGGGTCACTGGGGTG

CCTGGATGCCCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATCCCCTGCCGCTTTGACTTCCC

GGATGAGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTACTTCAATAGCCCCTACCCCAAGAACTACCCC

CCGGTGGTCTTCAAGTCGCGCACCCAAGTAGTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGG

ACCTGGGCCTGCGAAACTGCACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTACTT

CCGTGGGGACCTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGCGTCCTGGATATCGTCAACACC

CCCAACATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGAGGTCAGCTGCATGGTGCCGGACA

ACTGCCCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGGCCACGAGGGGCTGGGGGAGCCCGCTGTGCTGGG

CCGGCTGCGGGAGGACGAGGGCACCTGGGTGCAGGTGTCACTGCTGCACTTCGTGCCCACGAGGGAGGCC

AACGGCCACAGGCTGGGCTGCCAGGCCTCCTTCCCCAACACCACCCTGCAGTTCGAGGGCTACGCCAGCA

TGGACGTCAAGTACCCCCCGGTGATTGTGGAGATGAACTCCTCGGTGGAGGCCATCGAGGGCTCCCACGT

GAGCCTGCTCTGTGGGGCTGACAGCAACCCCCCGCCGCTGCTGACCTGGATGCGGGACGGGACAGTCCTC

CGGGAGGCGGTGGCCGAGAGCCTGCTCCTGGAGCTGGAGGAGGTGACCCCCGCCGAAGACGGCGTCTATG

CCTGCCTGGCCGAGAATGCCTATGGCCAGGACAACCGCACCGTGGGGCTCAGTGTCATGTATGCACCCTG

GAAGCCAACAGTGAACGGGACAATGGTGGCCGTAGAGGGGGAGACGGTCTCTATCTTGTGCTCCACACAG

AGCAACCCGGACCCTATTCTCACCATCTTCAAGGAGAAGCAGATCCTGTCCACGGTCATCTACGAGAGCG

AGCTGCAGCTGGAGCTGCCGGCCGTGTCACCCGAGGATGATGGAGAGTACTGGTGTGTGGCTGAGAACCA

GTATGGCCAGAGGGCCACCGCCTTCAACCTGTCTGTGGAGTTCGCCCCTGTGCTCCTCCTGGAGTCCCAC

TGCGCGGCAGCCCGAGACACGGTGCAGTGCCTGTGCGTGGTGAAGTCCAACCCGGAGCCGTCCGTGGCCT

TTGAGCTGCCATCGCGCAATGTGACCGTGAACGAGAGCGAGCGGGAGTTCGTGTACTCGGAGCGCAGCGG

CCTCGTGCTCACCAGCATCCTCACGCTGCGGGGGCAGGCCCAGGCCCCGCCCCGCGTCATCTGCACCGCG

AGGAACCTCTATGGCGCCAAGAGCCTGGAGCTGCCCTTCCAGGGAGCCCATCGACTGATGTGGGCCAAGA

TCGGGCCTGTGGGCGCCGTGGTCGCCTTTGCCATCCTGATTGCCATCGTCTGCTACATTACCCAGACACG

CAGGAAAAAGAACGTGACAGAGAGCCCCAGCTTCTCGGCAGGGGACAACCCTCCCGTCCTGTTCAGCAGC

GACTTCCGCATCTCTGGGGCACCAGAGAAGTACGAGAGCGAGAGGCGCCTGGGATCTGAGAGGAGGCTGC

TGGGCCTTCGGGGTGAGCCCCCAGAGCTGGACCTGAGCTATTCTCACTCGGACCTGGGGAAACGGCCCAC

CAAGGACAGCTACACGCTGACGGAGGAGCTAGCTGAGTATGCTGAAATCCGGGTCAAGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.