Human SIGLEC1/SN/CD169 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

SIGLEC1,SN (Sialoadhesin) or CD169 is a single-pass type I transmembrane protein belonging to the sialic acid binding Ig-like lectin (SIGLEC) family in the immunoglobulin superfamily (IgSF). SIGLECs are characterized by an N-terminal Ig-like V-type (IgV)domain that mediates sialic acid binding, followed by varying numbers (2 to 17) of Ig-like C2-type (IgC2) domains in the extracellular region, a transmembrane region, and a cytoplasmic tail with intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM). They can be classified into two subgroups: Siglecs-1, -2, and -4 subgroup, and a Siglec-3/CD33-related subgroup (Siglecs-3, and -5 through -13), defined by sequence similarity and clustered gene localization. SIGLECs are widely expressed on hematopoietic cells, often in a cell-type-specific manner.  SIGLEC1 contains 16 IgC2 domains and acts as an endocytic receptor mediating clathrin dependent endocytosis.  SIGLEC1 is a macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells.  SIGLEC1 preferentially binds to alpha-2,3-linked sialic acid and  SPN/CD43 on T-cells. SIGLEC1 may also play a role in hemopoiesis.  Alternative splicing produces a transcript variant encoding a soluble isoform of SIGLEC1.

 

 

Gene Symbol: SIGLEC1; SN; CD169; SIGLEC-1; Sialoadhesin; dJ1009E24.1

 

NCBI Gene ID: 6614

 

Uniprot Entry: Q9BZZ2

 

Construct Details: Full length human SIGLEC1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotics selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 5130 bp  

 

Gene Insert Sequence:  

ATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCATTCTTCCCAGCAGGCCAGGCCTCATGGGGCGTCT

CCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGC

CGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTG

AGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCG

AGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTT

CGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGG

GTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCT

ACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAA

CAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGAC

CACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAG

TGAAGTATGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCT

GGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGG

GTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACA

CCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCATGGCTGA

GGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAAT

GAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATA

CCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGG

CAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCAACCACCCGCCTCTCACTCCAGTCCTGACAGCCTTC

CTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGG

TGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGG

TACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAG

TGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGCCGCCCGTCTCC

TCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCC

CACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTC

CTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCA

GTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTACCCCCCTCGACAACCAACATTCACCACCAGGCT

GGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCC

CCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCA

GCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCA

CAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACC

TCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAG

AAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAA

TGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCC

CTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCT

ATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTG

CACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTG

GGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCC

GAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACAC

CTCACTCTTCTTCCAGGTCCGAGGAGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAG

GCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATG

GCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGC

CTATCATTGCCAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGCACCCATCAGCCTCCACGTG

TCCTATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCC

TGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCAC

CCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTG

CGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGG

GCCAGGCCTCGGCCTCAGCTGACTTCGACGCTCAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGT

GCGGGAGGGGCAGCTGGTGAACCTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACA

TGGTACCAGGATGGGCAGCAGCGCCTGGATGCCCACTCCATCCCCCTGCCCAACGTCACAGTCAGGGATG

CCACCTCCTACCGCTGCGGTGTGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGA

CGTCCTCTACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTG

GTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCT

CCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGG

TTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGGT

GTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACC

CTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGC

CTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAG

GGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTATGCCCCTCAGGACGCTGTCCTGTCCTCCT

TCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCT

GGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGC

CATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATG

TTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGCACGCGT

GGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGC

CCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGC

CCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCAC

TGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTC

TTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCC

TGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACAT

CCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAA

TACATCTGTTCTGCCTCAAATGTCCTGGGCTCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGC

ACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTT

GGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATTCGGTG

GAGATGGCTTTTCAGAAAGAGACCACGCAGCTCATTGATCCTGATGCAGCCACATGTGAGACCTCAACCT

GTGCCCCACCCCTGGGCTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.