Human SLAMF3 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

SLAMF3 (signaling lymphocytic activation molecule family member 3), also known as Ly9 and CD229, is a single-pass type I transmembrane glycoprotein that belongs to the SLAM subfamily of the CD2 protein family of the Ig (immunoglobulin) superfamily. Most SLAM family members function as adhesion molecules and co-receptors for lymphocyte activation and mediate tyrosine phosphorylation signals. SLAMF3 contains 2 Ig-like V-type and 2 Ig-like C2-type domains in the extracellular region and 2 ITSM (immunoreceptor tyrosine switch motifs) in the cytoplasmic region.  SLAMF3 is expressed on lymphocytes, thymocytes, and more weakly on NK cells. SLAMF3 cell surface expression on lymphocytes surface is differentially regulated within the SLAM family members. SLAMF3 is a pan-lymphocyte marker and antibodies against it are able to down-regulate T-cell activation. Homophilic interactions between SLAMF3 are mediated through its Ig­like domains and involved in the immunological synapse. Antigen stimulation of lymphocytes induces SLAMF3 clustering to the sites of T cell and B cell contact. Two tyrosines in the cytoplasmic domain are required for association with adaptor proteins and internalization. It has been reported that human and mouse SLAMF3 show cross­species binding.

 

 

Gene Symbol: SLAMF3; CD229; Ly9; hly9; mLY9; Ly-9; SLAMF-3; CDABP0070

 

NCBI Gene ID: 4063

 

Uniprot Entry: Q9HBG7

 

Construct Details: Full length human SLAMF3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1968 (bp)

 

Gene Insert Sequence:  

ATGGTGGCACCAAAGAGTCACACAGATGACTGGGCTCCTGGGCCTTTCTCCAGTAAGCCACAGAGGAGTCAGCTGCAAAT ATTCTCTTCTGTTCTACAGACCTCTCTCCTCTTCCTGCTCATGGGACTAAGAGCCTCTGGAAAGGACTCAGCCCCAACAG TGGTGTCAGGGATCCTAGGGGGTTCCGTGACTCTCCCCCTAAACATCTCAGTAGACACAGAGATTGAGAACGTCATCTGG ATTGGTCCCAAAAATGCTCTTGCTTTCGCACGTCCCAAAGAAAATGTAACCATTATGGTCAAAAGCTACCTGGGCCGACT AGACATCACCAAGTGGAGTTACTCCCTGTGCATCAGCAATCTGACTCTGAATGATGCAGGATCCTACAAAGCCCAGATAA ACCAAAGGAATTTTGAAGTCACCACTGAGGAGGAATTCACCCTGTTCGTCTATGAGCAGCTGCAGGAGCCCCAAGTCACC ATGAAGTCTGTGAAGGTGTCTGAGAACTTCTCCTGTAACATCACTCTAATGTGCTCCGTGAAGGGGGCAGAGAAAAGTGT TCTGTACAGCTGGACCCCAAGGGAACCCCATGCTTCTGAGTCCAATGGAGGCTCCATTCTTACCGTCTCCGGAACACCAT GTGACCCAGACCTGCCATACATCTGCACAGCCCAGAACCCCGTCAGCCAGAGAAGCTCCCTCCCTGTCCATGTTGGGCAG TTCTGTACAGATCCAGGAGCCTCCAGAGGAGGAACAACGGGGGAGACTGTGGTAGGGGTCCTGGGAGAGCCAGTCACCCT GCCACTTGCACTCCCAGCCTGCCGGGACACAGAGAAGGTTGTCTGGTTGTTTAACACATCCATCATTAGCAAAGAGAGGG AAGAAGCAGCAACGGCAGATCCACTCATTAAATCCAGGGATCCTTACAAGAACAGGGTGTGGGTCTCCAGCCAGGACTGC TCCCTGAAGATCAGCCAGCTGAAGATAGAGGACGCCGGCCCCTACCATGCCTACGTGTGCTCAGAGGCCTCCAGCGTCAC CAGCATGACACATGTCACCCTGCTCATCTACCGCAGGCTGAGGAAGCCCAAAATCACGTGGAGCCTCAGGCACAGTGAGG ATGGCATCTGCAGGATCAGCCTGACCTGCTCCGTGGAGGACGGGGGAAACACTGTCATGTACACATGGACCCCGCTGCAG AAGGAAGCTGTTGTGTCCCAAGGGGAATCACACCTCAATGTCTCATGGAGAAGCAGTGAAAATCACCCCAACCTCACATG CACAGCCAGCAACCCTGTCAGCAGGAGTTCCCACCAGTTTCTTTCTGAGAACATCTGTTCAGGACCTGAGAGAAACACAA AGCTTTGGATTGGGTTGTTCCTGATGGTTTGCCTTCTGTGCGTTGGGATCTTCAGCTGGTGCATTTGGAAGCGAAAAGGA CGGTGTTCAGTCCCAGCCTTCTGTTCCAGCCAAGCTGAGGCCCCAGCGGATACACCAGAACCCACAGCTGGCCACACGCT ATACTCTGTGCTCTCCCAAGGATATGAGAAGCTGGACACTCCCCTCAGGCCTGCCAGGCAACAGCCTACACCCACCTCAG ACAGCAGCTCTGACAGCAACCTCACAACTGAGGAGGATGAGGACAGGCCTGAGGTGCACAAGCCCATCAGTGGAAGATAT GAGGTGTTTGACCAGGTCACTCAGGAGGGCGCTGGACATGACCCAGCCCCTGAGGGCCAAGCAGACTATGATCCCGTCAC TCCATATGTCACGGAAGTTGAGTCTGTGGTTGGAGAGAACACCGTGTATGCACAAGTGTTCAACTTACAGGGAAAGACCC CAGTTTCTCAGAAGGAAGAGAGCTCAGCCACAATCTACTGCTCCATACGGAAACCTCAGGTGGTGCCACCACCACAACAG AATGATCTTGAGATTCCTGAAAGTCCTACCTATGAAAATTTCACCTGA

  

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Immunogenetics 11:65 (1980).   

2. J. Immunol. 125:2127 (1980).  

3. J. Immunol. 149:1636 (1992).  

4. Blood 97:3513 (2001).   

5. J Immunol. 174: 5977-86 (2005).

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0455-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.