Human PI16/CD359/CRISP9/PSPBP Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

MSMB is a member of the beta-microseminoprotein family and presumably an immunoglobulin binding factor (IGF). Members of the microseminoprotein protein family contain 10 conserved cysteine residues that form intra-molecular disulfide bonds. Specific receptors for MSMB are found on spermatozoa and in the prostate.  The expression of MSMB is found to be decreased in prostate cancer.  PI16 (peptidase inhibitor 16), CRSP9 or CD359 is a member of the CRSP (cysteine-rich secretory protein).  PI16  was identified as a secreted protein that interacts with MSMB/PSP94, thus also known as PSPBP.  PI16 may inhibit cardiomyocyte growth.  PI16 may serve as a marker following prostatectomy for prostate cancer. 

 

 

Gene Symbol: PI16; CD359; PSPBP; CRISP9; MSMBBP; CRSP-9; PI-16

 

NCBI Gene ID: 221476

 

Uniprot Entry: Q6UXB8

 

Construct Details: Full length human PI16 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1392 (bp)

 

Gene Insert Sequence:  

ATGCACGGCTCCTGCAGTTTCCTGATGCTTCTGCTGCCGCTACTGCTACTGCTGGTGGCCACCACAGGCC

CCGTTGGAGCCCTCACAGATGAGGAGAAACGTTTGATGGTGGAGCTGCACAACCTCTACCGGGCCCAGGT

ATCCCCGACGGCCTCAGACATGCTGCACATGAGATGGGACGAGGAGCTGGCCGCCTTCGCCAAGGCCTAC

GCACGGCAGTGCGTGTGGGGCCACAACAAGGAGCGCGGGCGCCGCGGCGAGAATCTGTTCGCCATCACAG

ACGAGGGCATGGACGTGCCGCTGGCCATGGAGGAGTGGCACCACGAGCGTGAGCACTACAACCTCAGCGC

CGCCACCTGCAGCCCAGGCCAGATGTGCGGCCACTACACGCAGGTGGTATGGGCCAAGACAGAGAGGATC

GGCTGTGGTTCCCACTTCTGTGAGAAGCTCCAGGGTGTTGAGGAGACCAACATCGAATTACTGGTGTGCA

ACTATGAGCCTCCGGGGAACGTGAAGGGGAAACGGCCCTACCAGGAGGGGACTCCGTGCTCCCAATGTCC

CTCTGGCTACCACTGCAAGAACTCCCTCTGTGAACCCATCGGAAGCCCGGAAGATGCTCAGGATTTGCCT

TACCTGGTAACTGAGGCCCCATCCTTCCGGGCGACTGAAGCATCAGACTCTAGGAAAATGGGTACTCCTT

CTTCCCTAGCAACGGGGATTCCGGCTTTCTTGGTAACAGAGGTCTCAGGCTCCCTGGCAACCAAGGCTCT

GCCTGCTGTGGAAACCCAGGCCCCAACTTCCTTAGCAACGAAAGACCCGCCCTCCATGGCAACAGAGGCT

CCACCTTGCGTAACAACTGAGGTCCCTTCCATTTTGGCAGCTCACAGCCTGCCCTCCTTGGATGAGGAGC

CAGTTACCTTCCCCAAATCGACCCATGTTCCTATCCCAAAATCAGCAGACAAAGTGACAGACAAAACAAA

AGTGCCCTCTAGGAGCCCAGAGAACTCTCTGGACCCCAAGATGTCCCTGACAGGGGCAAGGGAACTCCTA

CCCCATGCCCAGGAGGAGGCTGAGGCTGAGGCTGAGTTGCCTCCTTCCAGTGAGGTCTTGGCCTCAGTTT

TTCCAGCCCAGGACAAGCCAGGTGAGCTGCAGGCCACACTGGACCACACGGGGCACACCTCCTCCAAGTC

CCTGCCCAATTTCCCCAATACCTCTGCCACCGCTAATGCCACGGGTGGGCGTGCCCTGGCTCTGCAGTCG

TCCTTGCCAGGTGCAGAGGGCCCTGACAAGCCTAGCGTCGTGTCAGGGCTGAACTCGGGCCCTGGTCATG

TGTGGGGCCCTCTCCTGGGACTACTGCTCCTGCCTCCTCTGGTGTTGGCTGGAATCTTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.