Human SLAMF1 Full-length Gene in Lentivector, Endotoxin-free DNA

Description

SLAMF1 (signaling lymphocytic activation molecule family member1), also known as SLAM and CD150, is a single-pass type I transmembrane glycoprotein that belongs to the SLAM subfamily of the CD2 protein family of the Ig (immunoglobulin) superfamily. Most SLAM family members function as adhesion molecules and co-receptors for lymphocyte activation and mediate tyrosine phosphorylation signals. SLAMF1 contains 1 Ig-like V-type and 1 Ig-like C2-type domains in the extracellular region and 3 ITSM (immunoreceptor tyrosine switch motifs) in the cytoplasmic region. SLAMF1 is expressed on lymphocytes, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells.  Its expression is up­regulated on lymphocytes cells after activation. As a self-ligand, SLAMF1 plays an important role in bidirectional T-cell to B-cell stimulation.  It performs diverse immunologic functions including T/B-cell costimulation, induction of interferon-γ in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. SLAMF1 ligation also promotes an allergen­induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages. In human, SLAMF1 also functions as a cellular entry receptor for measles virus.

 

 

Gene Symbol: SLAMF1; CD150; SLAM; CDw150; IPO-3; SLAMF-1

 

NCBI Gene ID: 6504

 

Uniprot Entry: Q13291

 

Construct Details: Full length human SLAMF1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1008 (bp)

 

Gene Insert Sequence:  

ATGGATCCCAAGGGGCTCCTCTCCTTGACCTTCGTGCTGTTTCTCTCCCTGGCTTTTGGGGCAAGCTACGGAACAGGTGG

GCGCATGATGAACTGCCCAAAGATTCTCCGGCAGTTGGGAAGCAAAGTGCTGCTGCCCCTGACATATGAAAGGATAAATA

AGAGCATGAACAAAAGCATCCACATTGTCGTCACAATGGCAAAATCACTGGAGAACAGTGTCGAGAACAAAATAGTGTCT

CTTGATCCATCCGAAGCAGGCCCTCCACGTTATCTAGGAGATCGCTACAAGTTTTATCTGGAGAATCTCACCCTGGGGAT

ACGGGAAAGCAGGAAGGAGGATGAGGGATGGTACCTTATGACCCTGGAGAAAAATGTTTCAGTTCAGCGCTTTTGCCTGC

AGTTGAGGCTTTATGAGCAGGTCTCCACTCCAGAAATTAAAGTTTTAAACAAGACCCAGGAGAACGGGACCTGCACCTTG

ATACTGGGCTGCACAGTGGAGAAGGGGGACCATGTGGCTTACAGCTGGAGTGAAAAGGCGGGCACCCACCCACTGAACCC

AGCCAACAGCTCCCACCTCCTGTCCCTCACCCTCGGCCCCCAGCATGCTGACAATATCTACATCTGCACCGTGAGCAACC

CTATCAGCAACAATTCCCAGACCTTCAGCCCGTGGCCCGGATGCAGGACAGACCCCTCAGAAACAAAACCATGGGCAGTG

TATGCTGGGCTGTTAGGGGGTGTCATCATGATTCTCATCATGGTGGTAATACTACAGTTGAGAAGAAGAGGTAAAACGAA

CCATTACCAGACAACAGTGGAAAAAAAAAGCCTTACGATCTATGCCCAAGTCCAGAAACCAGGTCCTCTTCAGAAGAAAC

TTGACTCCTTCCCAGCTCAGGACCCTTGCACCACCATATATGTTGCTGCCACAGAGCCTGTCCCAGAGTCTGTCCAGGAA

ACAAATTCCATCACAGTCTATGCTAGTGTGACACTTCCAGAGAGCTGA

  

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. Nature 376: 260-263 (1995).

2. Nature 406: 893-897 (2000).

3. Nature Immunol. 4: 19-24 (2003).

4. J. Exp. Med. 185:993 (1997).  

5. J. Immunol. 158:4036 (1997).  

 

 

 

Product datasheet (pdf) can be downloaded here: LTP0465-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.