Human FZD8/Fz8/Frizzled8 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

Members of the Frizzled (FZD) gene family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site (Wnt) family of signaling proteins. Most FZD receptors are coupled to the beta-catenin signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt-target genes. A second signaling pathway involving PKC and calcium fluxes has been described for some family members, but it remains unclear if it represents a distinct pathway as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. In addition both pathways seem to involve interactions with G-proteins. The FZD protein typically contains a signal peptide, a cysteine-rich FZ (frizzled) domain in the N-terminal extracellular region, 7 transmembrane (7TM) domains, and a C-terminal cytoplasmic Lys-Thr-X-X-X-Trp motif that interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.  The FZ domain is involved in binding with Wnt ligands. FZD8 acts as a co-receptor along with RYK for Wnt proteins, such as WNT1.  FZD8 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The FZD8 gene is highly expressed in human cancer cell lines. 

 

 

Gene Symbol: FZD8; FZD-8; hFz8; Fz-8

 

NCBI Gene ID: 8325

 

Uniprot Entry: Q9H461

 

Construct Details: Full length human FZD8 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2085 (bp)

 

Gene Insert Sequence:  

ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTGCTGGCCGCCTTGGCGCTGCTGCAGCGCTCTAGCG

GCGCTGCGGCCGCCTCGGCCAAGGAGCTGGCATGCCAAGAGATCACCGTGCCGCTGTGTAAGGGCATCGG

CTACAACTACACCTACATGCCCAATCAGTTCAACCACGACACGCAAGACGAGGCGGGCCTGGAGGTGCAC

CAGTTCTGGCCGCTGGTGGAGATCCAGTGCTCGCCCGATCTCAAGTTCTTCCTGTGCAGCATGTACACGC

CCATCTGCCTAGAGGACTACAAGAAGCCGCTGCCGCCCTGCCGCTCGGTGTGCGAGCGCGCCAAGGCCGG

CTGCGCGCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGACCGCATGCGCTGCGACCGGCTGCCCGAG

CAAGGCAACCCTGACACGCTGTGCATGGACTACAACCGCACCGACCTAACCACCGCCGCGCCCAGCCCGC

CGCGCCGCCTGCCGCCGCCGCCGCCCGGCGAGCAGCCGCCTTCGGGCAGCGGCCACGGCCGCCCGCCGGG

GGCCAGGCCCCCGCACCGCGGCGGCGGCAGGGGCGGTGGCGGCGGGGACGCGGCGGCGCCCCCAGCTCGC

GGCGGCGGCGGTGGCGGGAAGGCGCGGCCCCCTGGCGGCGGCGCGGCTCCCTGCGAGCCCGGGTGCCAGT

GCCGCGCGCCTATGGTGAGCGTGTCCAGCGAGCGCCACCCGCTCTACAACCGCGTCAAGACAGGCCAGAT

CGCTAACTGCGCGCTGCCCTGCCACAACCCCTTTTTCAGCCAGGACGAGCGCGCCTTCACCGTCTTCTGG

ATCGGCCTGTGGTCGGTGCTCTGCTTCGTGTCCACCTTCGCCACCGTCTCCACCTTCCTTATCGACATGG

AGCGCTTCAAGTACCCGGAGCGGCCCATTATCTTCCTCTCGGCCTGCTACCTCTTCGTGTCGGTGGGCTA

CCTAGTGCGCCTGGTGGCGGGCCACGAGAAGGTGGCGTGCAGCGGTGGCGCGCCGGGCGCGGGGGGCGCT

GGGGGCGCGGGCGGCGCGGCGGCGGGCGCGGGCGCGGCGGGCGCGGGCGCGGGCGGCCCGGGCGGGCGCG

GCGAGTACGAGGAGCTGGGCGCGGTGGAGCAGCACGTGCGCTACGAGACCACCGGCCCCGCGCTGTGCAC

CGTGGTCTTCTTGCTGGTCTACTTCTTCGGCATGGCCAGCTCCATCTGGTGGGTGATCTTGTCGCTCACA

TGGTTCCTGGCGGCCGGTATGAAGTGGGGCAACGAAGCCATCGCCGGCTACTCGCAGTACTTCCACCTGG

CCGCGTGGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGCTCAGCTCGGTGGACGGCGACCCGGT

GGCGGGCATCTGCTACGTGGGCAACCAGAGCCTGGACAACCTGCGCGGCTTCGTGCTGGCGCCGCTGGTC

ATCTACCTCTTCATCGGCACCATGTTCCTGCTGGCCGGCTTCGTGTCCCTCTTCCGCATCCGCTCGGTCA

TCAAGCAACAGGACGGCCCCACCAAGACGCACAAGCTGGAGAAGCTGATGATCCGCCTGGGCCTGTTCAC

CGTGCTCTACACCGTGCCCGCCGCGGTGGTGGTCGCCTGCCTCTTCTACGAGCAGCACAACCGCCCGCGC

TGGGAGGCCACGCACAACTGCCCGTGCCTGCGGGACCTGCAGCCCGACCAGGCACGCAGGCCCGACTACG

CCGTCTTCATGCTCAAGTACTTCATGTGCCTAGTGGTGGGCATCACCTCGGGCGTGTGGGTCTGGTCCGG

CAAGACGCTGGAGTCCTGGCGCTCCCTGTGCACCCGCTGCTGCTGGGCCAGCAAGGGCGCCGCGGTGGGC

GGGGGCGCGGGCGCCACGGCCGCGGGGGGTGGCGGCGGGCCGGGGGGCGGCGGCGGCGGGGGACCCGGCG

GCGGCGGGGGGCCGGGCGGCGGCGGGGGCTCCCTCTACAGCGACGTCAGCACTGGCCTGACGTGGCGGTC

GGGCACGGCGAGCTCCGTGTCTTATCCAAAGCAGATGCCATTGTCCCAGGTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

  

 

Additional supporting documents, including product data sheet PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.