Human CD13/ANPEP/APN/LAP1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

CD13, also known as ANPEP (alanyl aminopeptidase), APM (aminopeptidase M) and APN (aminopeptidase N), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a 935 amino acid (aa) extracellular domain (ECD). The large ECD contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is a broad specificity aminopeptidase belonging to the Peptidase M1 family. CD13 may be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines.  CD13 located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. CD13 cleaves antigen peptides bound to major histocompatibility complex class II molecules of antigen presenting cells and to degrade neurotransmitters at synaptic junctions. CD13 Is also implicated as a regulator of IL-8 bioavailability in the endometrium, contributing to angiogenesis.  CD13 is a receptor for human coronavirus that is a cause of upper respiratory tract infections. It also mediates human cytomegalovirus (HCMV) infection. Defects in the CD13 gene may be a cause of various types of leukemia or lymphoma. CD13 plays a critical role in the pathogenesis of cholesterol gallstone disease. 

 

 

Gene Symbol: ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

 

NCBI Gene ID: 290

 

Uniprot Entry: P15144

 

Construct Details: Full length human CD13 is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2904 (bp)

 

Gene Insert Sequence:  

ATGGCCAAGGGCTTCTATATTTCCAAGTCCCTGGGCATCCTGGGGATCCTCCTGGGCGTGGCAGCCGTGT

GCACAATCATCGCACTGTCAGTGGTGTACTCCCAGGAGAAGAACAAGAACGCCAACAGCTCCCCCGTGGC

CTCCACCACCCCGTCCGCCTCAGCCACCACCAACCCCGCCTCGGCCACCACCTTGGACCAAAGTAAAGCG

TGGAATCGTTACCGCCTCCCCAACACGCTGAAACCCGATTCCTACCGGGTGACGCTGAGACCGTACCTCA

CCCCCAATGACAGGGGCCTGTACGTTTTTAAGGGCTCCAGCACCGTCCGTTTCACCTGCAAGGAGGCCAC

TGACGTCATCATCATCCACAGCAAGAAGCTCAACTACACCCTCAGCCAGGGGCACAGGGTGGTCCTGCGT

GGTGTGGGAGGCTCCCAGCCCCCCGACATTGACAAGACTGAGCTGGTGGAGCCCACCGAGTACCTGGTGG

TGCACCTCAAGGGCTCCCTGGTGAAGGACAGCCAGTATGAGATGGACAGCGAGTTCGAGGGGGAGTTGGC

AGATGACCTGGCGGGCTTCTACCGCAGCGAGTACATGGAGGGCAATGTCAGAAAGGTGGTGGCCACTACA

CAGATGCAGGCTGCAGATGCCCGGAAGTCCTTCCCATGCTTCGATGAGCCGGCCATGAAGGCCGAGTTCA

ACATCACGCTTATCCACCCCAAGGACCTGACAGCCCTGTCCAACATGCTTCCCAAAGGTCCCAGCACCCC

ACTTCCAGAAGACCCCAACTGGAATGTCACTGAGTTCCACACCACGCCCAAGATGTCCACGTACTTGCTG

GCCTTCATTGTCAGTGAGTTCGACTACGTGGAGAAGCAGGCATCCAATGGTGTCTTGATCCGGATCTGGG

CCCGGCCCAGTGCCATTGCGGCGGGCCACGGCGATTATGCCCTGAACGTGACGGGCCCCATCCTTAACTT

CTTTGCTGGTCATTATGACACACCCTACCCACTCCCAAAATCAGACCAGATTGGCCTGCCAGACTTCAAC

GCCGGCGCCATGGAGAACTGGGGACTGGTGACCTACCGGGAGAACTCCCTGCTGTTCGACCCCCTGTCCT

CCTCCAGCAGCAACAAGGAGCGGGTGGTCACTGTGATTGCTCATGAGCTGGCCCACCAGTGGTTCGGGAA

CCTGGTGACCATAGAGTGGTGGAATGACCTGTGGCTGAACGAGGGCTTCGCCTCCTACGTGGAGTACCTG

GGTGCTGACTATGCGGAGCCCACCTGGAACTTGAAAGACCTCATGGTGCTGAATGATGTGTACCGCGTGA

TGGCAGTGGATGCACTGGCCTCCTCCCACCCGCTGTCCACACCCGCCTCGGAGATCAACACGCCGGCCCA

GATCAGTGAGCTGTTTGACGCCATCTCCTACAGCAAGGGCGCCTCAGTCCTCAGGATGCTCTCCAGCTTC

CTGTCCGAGGACGTATTCAAGCAGGGCCTGGCGTCCTACCTCCACACCTTTGCCTACCAGAACACCATCT

ACCTGAACCTGTGGGACCACCTGCAGGAGGCTGTGAACAACCGGTCCATCCAACTCCCCACCACCGTGCG

GGACATCATGAACCGCTGGACCCTGCAGATGGGCTTCCCGGTCATCACGGTGGATACCAGCACGGGGACC

CTTTCCCAGGAGCACTTCCTCCTTGACCCCGATTCCAATGTTACCCGCCCCTCAGAATTCAACTACGTGT

GGATTGTGCCCATCACATCCATCAGAGATGGCAGACAGCAGCAGGACTACTGGCTGATAGATGTAAGAGC

CCAGAACGATCTCTTCAGCACATCAGGCAATGAGTGGGTCCTGCTGAACCTCAATGTGACGGGCTATTAC

CGGGTGAACTACGACGAAGAGAACTGGAGGAAGATTCAGACTCAGCTGCAGAGAGACCACTCGGCCATCC

CTGTCATCAATCGGGCACAGATCATTAATGACGCCTTCAACCTGGCCAGTGCCCATAAGGTCCCTGTCAC

TCTGGCGCTGAACAACACCCTCTTCCTGATTGAAGAGAGACAGTACATGCCCTGGGAGGCCGCCCTGAGC

AGCCTGAGCTACTTCAAGCTCATGTTTGACCGCTCCGAGGTCTATGGCCCCATGAAGAACTACCTGAAGA

AGCAGGTCACACCCCTCTTCATTCACTTCAGAAATAATACCAACAACTGGAGGGAGATCCCAGAAAACCT

GATGGACCAGTACAGCGAGGTTAATGCCATCAGCACCGCCTGCTCCAACGGAGTTCCAGAGTGTGAGGAG

ATGGTCTCTGGCCTTTTCAAGCAGTGGATGGAGAACCCCAATAATAACCCGATCCACCCCAACCTGCGGT

CCACCGTCTACTGCAACGCTATCGCCCAGGGCGGGGAGGAGGAGTGGGACTTCGCCTGGGAGCAGTTCCG

AAATGCCACACTGGTCAATGAGGCTGACAAGCTCCGGGCAGCCCTGGCCTGCAGCAAAGAGTTGTGGATC

CTGAACAGGTACCTGAGCTACACCCTGAACCCGGACTTAATCCGGAAGCAGGACGCCACCTCTACCATCA

TCAGCATTACCAACAACGTCATTGGGCAAGGTCTGGTCTGGGACTTTGTCCAGAGCAACTGGAAGAAGCT

TTTTAACGATTATGGTGGTGGCTCGTTCTCCTTCTCCAACCTCATCCAGGCAGTGACACGACGATTCTCC

ACCGAGTATGAGCTGCAGCAGCTGGAGCAGTTCAAGAAGGACAACGAGGAAACAGGCTTCGGCTCAGGCA

CCCGGGCCCTGGAGCAAGCCCTGGAGAAGACGAAAGCCAACATCAAGTGGGTGAAGGAGAACAAGGAGGT

GGTGCTCCAGTGGTTCACAGAAAACAGCAAATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.