Human LINGO1/LERN1/LRRN6A Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation. The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson's disease models.
Gene Symbol: LINGO1; LERN1; LRRN6A; UNQ201; UNQ201/PRO227
NCBI Gene ID: 84894
Uniprot Entry: Q96FE5
Construct Details: The recombinant human LINGO1-Fc is expressed as a 734-amino acid protein consisting of Cys42 - Thr548 region of LINGO1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing human LINGO1-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
CPPRCECSAQ DRAVLCHRKR FVAVPEGIPT ETRLLDLGKN RIKTLNQDEF ASFPHLEELE
70 80 90 100 110 120
LNENIVSAVE PGAFNNLFNL RTLGLRSNRL KLIPLGVFTG LSNLTKLDIS ENKIVILLDY
130 140 150 160 170 180
MFQDLYNLKS LEVGDNDLVY ISHRAFSGLN SLEQLTLEKC NLTSIPTEAL SHLHGLIVLR
190 200 210 220 230 240
LRHLNINAIR DYSFKRLYRL KVLEISHWPY LDTMTPNCLY GLNLTSLSIT HCNLTAVPYL
250 260 270 280 290 300
AVRHLVYLRF LNLSYNPIST IEGSMLHELL RLQEIQLVGG QLAVVEPYAF RGLNYLRVLN
310 320 330 340 350 360
VSGNQLTTLE ESVFHSVGNL ETLILDSNPL ACDCRLLWVF RRRWRLNFNR QQPTCATPEF
370 380 390 400 410 420
VQGKEFKDFP DVLLPNYFTC RRARIRDRKA QQVFVDEGHT VQFVCRADGD PPPAILWLSP
430 440 450 460 470 480
RKHLVSAKSN GRLTVFPDGT LEVRYAQVQD NGTYLCIAAN AGGNDSMPAH LHVRSYSPDW
490 500 510 520 530 540
PHQPNKTFAF ISNQPGEGEA NSTRATVDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI
550 560 570 580 590 600
SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW
610 620 630 640 650 660
LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY
670 680 690 700 710 720
PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH
730
NHYTQKSLSL SPGK
M.W.: Calculated molecular mass (kDa): 83.1; Estimated by SDS-PAGE under reducing condition (kDa): 85-90
Calculated PI: 8.02
Calculated extinction coefficients (M-1 cm-1, at 280nm): 87875
Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
1. Eur. J. Neurosci. 18:3167 (2003)
2. Nat. Neurosci. 7:221-228 (2004)
3. Nat. Neurosci. 8:745-751 (2005)
4. Neuron 45:345-351 (2005)
5. J. Biol. Chem. 281:36378 (2006)
6. Proc. Natl. Acad. Sci. 104:14430 (2007)
Additional supporting documents, including product datasheet, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
Human LINGO2/LERN3/LRRN6C Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA