Human MRC1/CD206/MMR/CLEC13D Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

MRC1, also known as MMR (macrophage mannose receptor 1), CD206 and CLEC13D (C-type lectin domain family 13 member D), is a single-pass type I transmembrane protein and a member of the mannose receptor family of the C-type lectin superfamily.  MRC1 contains  8 C-type lectin domains, 1 fibronectin type-II domain and 1 ricin B-type lectin domain in the extracellular region. MRC1 mediates the endocytosis of glycoproteins by macrophages. MRC1 binds both sulfated and non-sulfated polysaccharide chains. MRC1 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. MRC1 acts as phagocytic receptor for bacteria, fungi and other pathogens. It also functions as a receptor for Dengue virus envelope protein E and interacts with hepatitis B virus envelope protein.

 

 

Gene Symbol: MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1

 

NCBI Gene ID: 4360

 

Uniprot Entry: P22897

 

Construct Details: Full length human CD206 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4371 (bp)

 

Gene Insert Sequence:  

ATGAGGCTACCCCTGCTCCTGGTTTTTGCCTCTGTCATTCCGGGTGCTGTTCTCCTACTGGACACCAGGC

AATTTTTAATCTATAATGAAGATCACAAGCGCTGCGTGGATGCAGTGAGTCCCAGTGCCGTCCAAACCGC

AGCTTGCAACCAGGATGCCGAATCACAGAAATTCCGATGGGTGTCCGAATCTCAGATTATGAGTGTTGCA

TTTAAATTATGCCTGGGAGTGCCATCAAAAACGGACTGGGTTGCTATCACTCTCTATGCCTGTGACTCAA

AAAGTGAATTTCAGAAATGGGAGTGCAAAAATGACACACTTTTGGGGATCAAAGGAGAAGATTTATTTTT

TAACTACGGCAACAGACAAGAAAAGAATATTATGCTCTACAAGGGATCGGGTTTATGGAGCAGGTGGAAG

ATCTATGGAACCACAGACAATCTGTGCTCCAGAGGTTATGAAGCCATGTATACGCTACTAGGCAATGCCA

ATGGAGCAACCTGTGCATTCCCGTTCAAGTTTGAAAACAAGTGGTACGCAGATTGCACGAGTGCTGGGCG

GTCGGATGGATGGCTCTGGTGCGGAACCACTACTGACTATGACACAGACAAGCTATTTGGATATTGTCCA

TTGAAATTTGAGGGCAGTGAAAGCTTATGGAATAAAGACCCGCTGACCAGCGTTTCCTACCAGATAAACT

CCAAATCCGCTTTAACGTGGCACCAGGCGAGGAAAAGCTGCCAACAACAGAACGCTGAGCTCCTGAGCAT

CACAGAGATTCATGAGCAAACATACCTGACAGGATTAACCAGTTCCTTGACCTCAGGACTCTGGATTGGA

CTTAACAGTCTGAGCTTCAACAGCGGTTGGCAGTGGAGTGACCGCAGTCCTTTCCGATATTTGAACTGGT

TACCAGGAAGTCCATCAGCTGAACCTGGAAAAAGCTGTGTGTCACTAAATCCTGGAAAAAATGCTAAATG

GGAAAATCTGGAATGTGTTCAGAAACTGGGCTATATTTGCAAAAAGGGCAACACCACTTTAAATTCTTTT

GTTATTCCCTCAGAAAGTGATGTGCCTACTCACTGTCCTAGTCAGTGGTGGCCGTATGCCGGTCACTGTT

ACAAGATTCACAGAGATGAGAAAAAAATCCAGAGGGATGCTCTGACCACCTGCAGGAAGGAAGGCGGTGA

CCTCACAAGTATCCACACCATCGAGGAATTGGACTTTATTATCTCCCAGCTAGGATATGAGCCAAATGAC

GAATTGTGGATCGGCTTAAATGACATTAAGATTCAAATGTACTTTGAGTGGAGTGATGGGACCCCTGTAA

CGTTTACCAAATGGCTTCGTGGAGAACCAAGCCATGAAAACAACAGACAGGAGGATTGTGTGGTGATGAA

AGGCAAGGATGGGTACTGGGCAGATCGGGGCTGTGAGTGGCCTCTTGGCTACATCTGCAAGATGAAATCA

CGAAGCCAAGGTCCAGAAATAGTGGAAGTCGAAAAAGGCTGCAGGAAAGGCTGGAAAAAACATCACTTTT

ACTGCTATATGATTGGACATACGCTTTCAACATTTGCAGAAGCAAACCAAACCTGTAATAATGAGAATGC

TTATTTAACAACTATTGAAGACAGATATGAACAAGCCTTCCTGACTAGTTTCGTTGGCTTAAGGCCTGAA

AAATATTTCTGGACAGGACTTTCAGATATACAAACCAAAGGGACTTTTCAGTGGACCATCGAGGAAGAGG

TTCGGTTCACCCACTGGAATTCAGATATGCCAGGGCGAAAGCCAGGGTGTGTTGCCATGAGAACCGGGAT

TGCAGGGGGCTTATGGGATGTTTTGAAATGTGATGAAAAGGCAAAATTTGTGTGCAAGCACTGGGCAGAA

GGAGTAACCCACCCACCGAAGCCCACGACGACTCCCGAACCCAAATGTCCGGAGGATTGGGGCGCCAGCA

GTAGAACAAGCTTGTGTTTCAAGCTGTATGCAAAAGGAAAACATGAGAAGAAAACGTGGTTTGAATCTCG

AGATTTTTGTCGAGCTCTGGGTGGAGACTTAGCTAGCATCAATAACAAAGAGGAACAGCAAACAATATGG

CGATTAATAACAGCTAGTGGAAGCTACCACAAACTGTTTTGGTTGGGATTGACATATGGAAGCCCTTCAG

AAGGTTTTACTTGGAGTGATGGTTCTCCTGTTTCATATGAAAACTGGGCTTATGGAGAACCTAATAATTA

TCAAAATGTTGAATACTGTGGTGAGCTGAAAGGTGACCCTACTATGTCTTGGAATGATATTAATTGTGAA

CACCTTAACAACTGGATTTGCCAGATACAAAAAGGACAAACACCAAAACCTGAGCCAACACCAGCTCCTC

AAGACAATCCACCAGTTACTGAAGATGGGTGGGTTATTTACAAAGACTACCAGTATTATTTCAGCAAAGA

GAAGGAAACCATGGACAATGCGCGAGCGTTTTGCAAGAGGAATTTTGGTGATCTTGTTTCTATTCAAAGT

GAAAGTGAAAAGAAGTTTCTATGGAAATATGTAAACAGAAATGATGCACAGTCTGCATATTTTATTGGTT

TATTGATCAGCTTGGATAAAAAGTTTGCTTGGATGGATGGAAGCAAAGTGGATTACGTGTCTTGGGCCAC

AGGTGAACCCAATTTTGCAAATGAAGATGAAAACTGTGTGACCATGTATTCAAATTCAGGGTTTTGGAAT

GACATTAACTGTGGCTATCCAAACGCCTTCATTTGCCAGCGACATAACAGTAGTATCAATGCTACCACAG

TTATGCCTACCATGCCCTCGGTCCCATCAGGGTGCAAGGAAGGTTGGAATTTCTACAGCAACAAGTGTTT

CAAAATCTTTGGATTTATGGAAGAAGAAAGAAAAAATTGGCAAGAGGCACGAAAAGCTTGTATAGGCTTT

GGAGGGAATCTGGTCTCCATACAAAATGAAAAAGAGCAAGCATTTCTTACCTATCACATGAAGGACTCCA

CTTTCAGTGCCTGGACTGGGCTGAATGATGTCAATTCAGAACACACGTTCCTTTGGACGGATGGACGAGG

AGTCCATTACACAAACTGGGGGAAAGGTTACCCTGGTGGAAGAAGAAGCAGTCTTTCTTATGAAGATGCT

GACTGTGTTGTTATTATTGGAGGTGCATCAAATGAAGCAGGAAAATGGATGGATGATACCTGCGACAGTA

AACGAGGCTACATATGCCAGACACGATCCGACCCTTCCTTGACTAATCCTCCAGCAACGATTCAAACAGA

TGGCTTTGTTAAATATGGCAAAAGCAGCTATTCACTCATGAGACAAAAATTTCAATGGCATGAAGCGGAG

ACATACTGCAAGCTTCACAATTCCCTTATAGCCAGCATTCTGGATCCCTACAGTAATGCATTTGCGTGGC

TGCAGATGGAAACATCTAATGAACGTGTGTGGATCGCCCTGAACAGTAACTTGACTGATAATCAATACAC

TTGGACTGATAAGTGGAGGGTGAGGTACACTAACTGGGCTGCTGATGAGCCCAAATTGAAATCAGCATGT

GTTTATCTGGATCTTGATGGCTACTGGAAGACAGCACATTGCAATGAAAGTTTTTACTTTCTCTGTAAAA

GATCAGATGAAATCCCTGCTACTGAACCCCCACAACTGCCTGGCAGATGCCCGGAGTCAGATCACACAGC

ATGGATTCCTTTCCATGGTCACTGTTACTATATTGAGTCCTCATATACAAGAAACTGGGGCCAAGCTTCT

CTGGAATGTCTTCGAATGGGTTCCTCTCTGGTTTCCATTGAAAGTGCTGCAGAATCCAGTTTTCTGTCAT

ATCGGGTTGAGCCACTTAAAAGTAAAACCAATTTTTGGATAGGATTGTTCAGAAATGTTGAAGGGACGTG

GCTGTGGATAAATAACAGTCCGGTCTCCTTTGTCAACTGGAACACAGGAGATCCCTCTGGTGAACGGAAT

GATTGTGTAGCTTTACATGCGTCTTCTGGGTTTTGGAGTAATATTCACTGTTCATCCTACAAAGGATATA

TTTGTAAAAGACCAAAAATTATTGATGCTAAACCTACTCATGAATTACTTACAACAAAAGCTGACACAAG

GAAGATGGACCCTTCTAAACCGTCTTCCAACGTGGCCGGAGTAGTCATCATTGTGATCCTCCTGATTTTA

ACGGGTGCTGGCCTTGCCGCCTATTTCTTTTATAAGAAAAGACGTGTGCACCTACCTCAAGAGGGCGCCT

TTGAAAACACTCTGTATTTTAACAGTCAGTCAAGCCCAGGAACTAGTGATATGAAAGATCTCGTGGGCAA

TATTGAACAGAATGAACACTCGGTCATCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.