Human LILRA4/CD85g/ILT7 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

LILRA4 (Leukocyte immunoglobulin like receptor A4), also known as CD85g and ILT7 (Immunoglobulin-like transcript 7), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  LIR family members are known to have activating and inhibitory functions in leukocytes.  LILRA4 belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail.LILRA3 contains 4 Ig-like C2 type domains in the extracellular region. LILRA4 is expressed predominantly on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response. Expression of the LILRA4 gene is down-regulated by interleukin 3 (IL3).  LILRA4 may act as receptor for class I MHC antigens. Ligand binding leads to the activation of eosinophils and to the release of RNASE2, IL4 and leukotriene C4.

 

 

Gene Symbol: LILRA4; ILT7; CD85G; ILT-7

 

NCBI Gene ID: 23547

 

Uniprot Entry: P59901

 

Construct Details: Full length human LILRA4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1500 (bp)

 

Gene Insert Sequence:  

ATGACCCTCATTCTCACAAGCCTGCTCTTCTTTGGGCTGAGCCTGGGCCCCAGGACCCGGGTGCAGGCAG

AAAACCTACTCAAACCCATCCTGTGGGCCGAGCCAGGTCCCGTGATCACCTGGCATAACCCCGTGACCAT

CTGGTGTCAGGGCACCCTGGAGGCCCAGGGGTACCGTCTGGATAAAGAGGGAAACTCAATGTCGAGGCAC

ATATTAAAAACACTGGAGTCTGAAAACAAGGTCAAACTCTCCATCCCATCCATGATGTGGGAACATGCAG

GGCGATATCACTGTTACTATCAGAGCCCTGCAGGCTGGTCAGAGCCCAGCGACCCCCTGGAGCTGGTGGT

GACAGCCTACAGCAGACCCACCCTGTCCGCACTGCCAAGCCCTGTGGTGACCTCAGGAGTGAACGTGACC

CTCCGGTGTGCCTCACGGCTGGGACTGGGCAGGTTCACTCTGATTGAGGAAGGAGACCACAGGCTCTCCT

GGACCCTGAACTCACACCAACACAACCATGGAAAGTTCCAGGCCCTGTTCCCCATGGGCCCCCTGACCTT

CAGCAACAGGGGTACATTCAGATGCTACGGCTATGAAAACAACACCCCATACGTGTGGTCGGAACCCAGT

GACCCCCTGCAGCTACTGGTGTCAGGCGTGTCTAGGAAGCCCTCCCTCCTGACCCTGCAGGGCCCTGTCG

TGACCCCCGGAGAGAATCTGACCCTCCAGTGTGGCTCTGATGTCGGCTACATCAGATACACTCTGTACAA

GGAGGGGGCCGATGGCCTCCCCCAGCGCCCTGGCCGGCAGCCCCAGGCTGGGCTCTCCCAGGCCAACTTC

ACCCTGAGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTACGGCGCACACAACGTCTCCTCCG

AGTGGTCGGCCCCCAGTGACCCCCTGGATATCCTGATCGCAGGACAGATCTCTGACAGACCCTCCCTCTC

AGTGCAGCCGGGCCCCACGGTGACCTCAGGAGAGAAGGTGACCCTGCTGTGTCAGTCATGGGACCCGATG

TTCACTTTCCTTCTGACCAAGGAGGGGGCAGCCCATCCCCCGTTGCGTCTGAGATCAATGTACGGAGCTC

ATAAGTACCAGGCTGAATTCCCCATGAGTCCTGTGACCTCAGCCCACGCGGGGACCTACAGGTGCTACGG

CTCACGCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGAGCCCCTGGAGCTCGTGGTCTCAGGAGCA

ACTGAGACCCTCAATCCAGCACAAAAGAAGTCAGATTCCAAGACTGCCCCACACCTCCAGGATTACACAG

TGGAGAATCTCATCCGCATGGGTGTGGCTGGCTTGGTCCTGCTGTTCCTCGGGATTCTGTTATTTGAGGC

TCAGCACAGCCAGAGAAGCCCCCCAAGGTGCAGCCAGGAGGCAAACAGCAGAAAGGACAATGCACCCTTC

AGAGTGGTGGAGCCTTGGGAACAGATCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.