Human KLRG1/CLEC15A/MAFAL/2F1 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

KLRG1 belongs to the killer cell lectin-like receptor (KLR) or NKG2 family of the C-type lectin superfamily, which is a group of single pass type II transmembrane proteins preferentially expressed in natural killer (NK) cells.  NK cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells regulate specific humoral and cell-mediated immunity. The KLR/NKG2 family is characterized by the presence of a C-type lectin domain in the extracellular region at the C-terminus. KLRG1 forms a disulfide-bond linked  homodimer. KLRG1 is expressed specifically on natural killer (NK) cells and T-cells, mainly CD8 T-cell. KLRG1 contains 1 copy of a cytoplasmic motif referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM) involved in modulation of cellular responses. KLRG1 plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. KLRG1 may mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells. 

  

 

Gene Symbol: KLRG1; 2F1; MAFA; MAFA-L; CLEC15A; MAFA-2F1; MAFA-LIKE

 

NCBI Gene ID: 10219

 

Uniprot Entry: Q96E93

 

Construct Details: Full length human KLRG1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 588 (bp)

 

Gene Insert Sequence:  

ATGACTGACAGTGTTATTTATTCCATGTTAGAGTTGCCTACGGCAACCCAAGCCCAGAATGACTATGGACCACAGCAAAA

ATCTTCCTCTTCCAGGCCTTCTTGTTCTTGCCTTGTGGCAATAGCTTTGGGGCTTCTGACTGCAGTTCTTCTGAGTGTGC

TGCTATACCAGTGGATCCTGTGCCAGGGCTCCAACTACTCCACTTGTGCCAGCTGTCCTAGCTGCCCAGACCGCTGGATG

AAATATGGTAACCATTGTTATTATTTCTCAGTGGAGGAAAAGGACTGGAATTCTAGTCTGGAATTCTGCCTAGCCAGAGA

CTCACACCTCCTTGTGATAACGGACAATCAGGAAATGAGCCTGCTCCAAGTTTTCCTCAGTGAGGCCTTTTGCTGGATTG

GTCTGAGGAACAATTCTGGCTGGAGGTGGGAAGATGGATCACCTCTAAACTTCTCAAGGATTTCTTCTAATAGCTTTGTG

CAGACATGCGGTGCCATCAACAAAAATGGTCTTCAAGCCTCAAGCTGTGAAGTTCCTTTACACTGGGTGTGTAAGAAGTG

TCCCTTTGCAGATCAAGCTTTATTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.