Human SLC39A7/KE4/HKE4/ZIP7/RING5 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

SLC39A7 (solute carrier family 39 member 7), also known as Zinc transporter ZIP7, is a six-pass transmembrane protein belonging to the ZIP transporter (TC 2.A.5) family. SLC39A7 shows structural characteristics of zinc transporters and may act as a zinc-influx transporter.  Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A7 transports Zn2+ from the endoplasmic reticulum/Golgi apparatus to the cytosol. Transport is stimulated by growth factors, such as EGF, and Ca2+, as well as by exogenous Zn2+.  This transport may be important for activation of tyrosine kinases, some of which could be involved in cancer progression. Modulation of SLC39A7 could be useful as a therapeutic agent against cancer. 

 

 

Gene Symbol: SLC39A7; KE4; HKE4; ZIP7; hZIP7; ZIP-7; RING5; H2-KE4; D6S115E; D6S2244E

 

NCBI Gene ID: 7922

 

Uniprot Entry: O92504

 

Construct Details: Full length human SLC39A7 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1410(bp)

 

Gene Insert Sequence:  

ATGGCCAGAGGCCTGGGGGCCCCCCACTGGGTGGCCGTGGGACTGCTGACCTGGGCGACCTTGGGGCTTC

TGGTGGCTGGACTCGGGGGTCATGACGACCTGCACGACGATCTGCAAGAGGACTTCCATGGCCACAGCCA

CAGGCACTCACATGAAGATTTCCACCATGGCCACAGCCATGCCCATGGCCATGGCCACACTCACGAGAGC

ATCTGGCATGGACATACCCACGATCACGACCATGGACATTCACATGAGGATTTACACCATGGCCATAGCC

ATGGCTACTCCCATGAGAGCCTCTACCACAGAGGACATGGACATGACCATGAGCATAGCCATGGAGGCTA

TGGGGAGTCTGGGGCTCCAGGCATCAAGCAGGACCTGGATGCTGTCACTCTCTGGGCTTATGCACTGGGG

GCCACAGTGCTGATCTCAGCAGCTCCATTTTTTGTCCTCTTCCTTATCCCCGTGGAGTCGAACTCTCCCC

GGCATCGCTCTCTACTTCAGATCTTGCTCAGTTTTGCTTCCGGTGGGCTCCTGGGAGATGCTTTCCTGCA

CCTCATTCCTCATGCTCTTGAACCTCATTCTCACCACACTCTGGAGCAACCCGGACATGGACACTCCCAC

AGTGGCCAGGGCCCCATTCTGTCTGTGGGACTGTGGGTTCTCAGTGGAATTGTTGCCTTTCTTGTCGTGG

AGAAATTTGTGAGACATGTGAAAGGAGGACATGGTCACAGTCATGGACATGGACACGCTCACAGTCATAC

ACGTGGAAGTCATGGACATGGAAGACAAGAGCGTTCTACCAAGGAGAAGCAGAGCTCAGAGGAAGAAGAA

AAGGAAACAAGAGGGGTTCAGAAGAGGCGAGGAGGGAGCACAGTACCCAAAGATGGGCCAGTGAGACCTC

AGAACGCTGAAGAAGAAAAAAGAGGCTTAGACCTGCGTGTGTCGGGGTACCTGAATCTGGCTGCTGACTT

GGCACACAACTTCACTGATGGTCTGGCCATTGGGGCTTCCTTTCGAGGGGGCCGGGGACTAGGGATCCTG

ACCACAATGACTGTCCTGCTACATGAAGTGCCCCACGAGGTCGGAGACTTTGCCATCTTGGTCCAGTCTG

GCTGCAGCAAAAAGCAGGCGATGCGTCTGCAACTACTGACAGCAGTAGGGGCACTGGCAGGCACAGCCTG

TGCCCTTCTCACTGAAGGAGGAGCAGTGGGCAGTGAAATTGCAGGTGGTGCAGGTCCTGGCTGGGTCCTG

CCATTTACTGCAGGTGGCTTTATCTACGTAGCAACAGTGTCTGTGTTGCCCGAGCTGCTGAGGGAGGCAT

CACCATTGCAATCACTTCTGGAGGTGCTGGGGCTGCTGGGGGGAGTTATCATGATGGTGCTGATTGCCCA

CCTTGAGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.