Human ULBP3/N2DL3/RAET1N/NKG2DL3 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

ULBP3 (UL16 binding protein 3) is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. ULBP3 is a GPI-anchored glycoprotein that belongs to the MHC-I family of Immunoglobulin superfamily (IgSF).  Binding of the ligand including ULBP1, ULBP2 and ULBP3, to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt, resulting in the production of cytokines and chemokines. ULBP3 does not bind to beta2-microglobulin but interacts with human cytomegalovirus/HHV-5 protein UL142. ULBP3 has lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.

 

 

Gene Symbol: ULBP3; N2DL-3; RAET1N; NKG2DL3; ALCAN-gamma

 

NCBI Gene ID: 79465

 

Uniprot Entry: Q9BZM4

 

Construct Details: Full length human ULBP3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 735 (bp)

 

Gene Insert Sequence:  

ATGGCAGCGGCCGCCAGCCCCGCGATCCTTCCGCGCCTCGCGATTCTTCCGTACCTGCTATTCGACTGGT

CCGGGACGGGGCGGGCCGACGCTCACTCTCTCTGGTATAACTTCACCATCATTCATTTGCCCAGACATGG

GCAACAGTGGTGTGAGGTCCAGAGCCAGGTGGATCAGAAGAATTTTCTCTCCTATGACTGTGGCAGTGAC

AAGGTCTTATCTATGGGTCACCTAGAAGAGCAGCTGTATGCCACAGATGCCTGGGGAAAACAACTGGAAA

TGCTGAGAGAGGTGGGGCAGAGGCTCAGACTGGAACTGGCTGACACTGAGCTGGAGGATTTCACACCCAG

TGGACCCCTCACGCTGCAGGTCAGGATGTCTTGTGAGTGTGAAGCCGATGGATACATCCGTGGATCTTGG

CAGTTCAGCTTCGATGGACGGAAGTTCCTCCTCTTTGACTCAAACAACAGAAAGTGGACAGTGGTTCACG

CTGGAGCCAGGCGGATGAAAGAGAAGTGGGAGAAGGATAGCGGACTGACCACCTTCTTCAAGATGGTCTC

AATGAGAGACTGCAAGAGCTGGCTTAGGGACTTCCTGATGCACAGGAAGAAGAGGCTGGAACCCACAGCA

CCACCCACCATGGCCCCAGGCTTAGCTCAACCCAAAGCCATAGCCACCACCCTCAGTCCCTGGAGCTTCC

TCATCATCCTCTGCTTCATCCTCCCTGGCATCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.