Human MR1 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

MR1 is a major histocompatibility complex class I-related gene protein.  It is a single pass type I transmembrane glycoprotein that belongs to the MHC-I family of Immunoglobulin superfamily (IgSF). MR1 contains 1 Ig-like C1-type motifs (1 IgC1) in the extracellular domain (ECD) in addition to a transmembrane (TM) domain and a cytoplasmic tail. MR1 heterodimerizes with B2M, this interaction is required for surface expression.  MR1 is an antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. It is involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT).  MAIT lymphocytes represent a small population of T-cells primarily found in the gut. The presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. 

 

 

Gene Symbol: MR1; MR-1; HLALS

 

NCBI Gene ID: 3140

 

Uniprot Entry: Q95460

 

Construct Details: Full length human MR1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1026 (bp)

 

Gene Insert Sequence:  

ATGGGGGAACTGATGGCGTTCCTGTTACCTCTCATCATTGTGTTAATGGTGAAGCACAGCGATTCCCGGA

CGCACTCTCTGAGATATTTTCGCCTGGGCGTTTCGGATCCCATCCATGGGGTCCCTGAATTTATTTCGGT

TGGGTACGTGGACTCGCACCCTATCACCACATATGACAGTGTCACTCGGCAGAAGGAGCCACGGGCCCCA

TGGATGGCAGAGAACCTCGCGCCTGATCACTGGGAGAGGTACACTCAGCTGCTGAGGGGCTGGCAGCAGA

TGTTCAAGGTGGAACTGAAGCGCCTACAGAGGCACTACAATCACTCAGGGTCTCACACTTACCAGAGAAT

GATTGGCTGTGAGCTGCTGGAGGATGGAAGCACCACAGGATTTCTGCAGTATGCATATGACGGGCAGGAT

TTCCTGATCTTCAATAAAGACACCCTCTCCTGGCTGGCTGTAGATAATGTGGCTCACACCATCAAGCAGG

CATGGGAGGCCAATCAGCATGAGTTGCTGTATCAAAAGAATTGGCTGGAAGAAGAATGTATTGCCTGGCT

AAAGAGATTCCTGGAGTATGGGAAAGACACCCTACAAAGAACAGAGCCCCCACTGGTCAGAGTAAATCGC

AAAGAAACTTTTCCAGGGGTTACAGCTCTCTTCTGCAAAGCTCATGGCTTTTACCCCCCAGAAATTTACA

TGACATGGATGAAAAACGGGGAAGAAATTGTCCAAGAAATTGATTATGGAGACATTCTTCCCAGTGGGGA

TGGAACCTATCAGGCGTGGGCATCAATTGAGCTTGATCCTCAGAGCAGCAACCTTTACTCCTGTCATGTG

GAGCACTGCGGTGTCCACATGGTTCTTCAGGTCCCCCAGGAATCAGAAACTATCCCTCTTGTGATGAAAG

CTGTCTCTGGGTCCATTGTCCTTGTCATTGTGCTGGCTGGAGTTGGTGTTCTAGTCTGGAGAAGAAGGCC

CCGAGAGCAAAATGGAGCCATCTACCTTCCAACACCAGATCGATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.