Human CD105/Endoglin/ENG/END/HHT1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
CD105 or Endoglin (ENG) is a single-pass type I transmembrane glycoprotein. It is a homodimer and a major glycoprotein of the vascular endothelium. CD105 is involved in the regulation of angiogenesis and plays a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 is a also component of the transforming growth factor beta receptor (TGFBR)complex and it binds to the beta1 and beta3 peptides with high affinity. CD105 acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. CD105 is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia (HHT1), also known as Osler-Rendu-Weber syndrome 1 (ORW1), an autosomal dominant multisystemic vascular dysplasia. The CD105 gene is also implicated in preeclampsia and several types of cancer.
Gene Symbol: CD105; Endoglin; ENG; END; HHT1; ORW1
NCBI Gene ID: 2022
Uniprot Entry: P17813
Construct Details: The recombinant human CD105-Fc fusion is expressed as a 789 amino acid protein consisting of Glu26 - Gly586 region of CD105 and a Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing human CD105-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
ETVHCDLQPV GPERGEVTYT TSQVSKGCVA QAPNAILEVH VLFLEFPTGP SQLELTLQAS
70 80 90 100 110 120
KQNGTWPREV LLVLSVNSSV FLHLQALGIP LHLAYNSSLV TFQEPPGVNT TELPSFPKTQ
130 140 150 160 170 180
ILEWAAERGP ITSAAELNDP QSILLRLGQA QGSLSFCMLE ASQDMGRTLE WRPRTPALVR
190 200 210 220 230 240
GCHLEGVAGH KEAHILRVLP GHSAGPRTVT VKVELSCAPG DLDAVLILQG PPYVSWLIDA
250 260 270 280 290 300
NHNMQIWTTG EYSFKIFPEK NIRGFKLPDT PQGLLGEARM LNASIVASFV ELPLASIVSL
310 320 330 340 350 360
HASSCGGRLQ TSPAPIQTTP PKDTCSPELL MSLIQTKCAD DAMTLVLKKE LVAHLKCTIT
370 380 390 400 410 420
GLTFWDPSCE AEDRGDKFVL RSAYSSCGMQ VSASMISNEA VVNILSSSSP QRKKVHCLNM
430 440 450 460 470 480
DSLSFQLGLY LSPHFLQASN TIEPGQQSFV QVRVSPSVSE FLLQLDSCHL DLGPEGGTVE
490 500 510 520 530 540
LIQGRAAKGN CVSLLSPSPE GDPRFSFLLH FYTVPIPKTG TLSCTVALRP KTGSQDQEVH
550 560 570 580 590 600
RTVFMRLNII SPDLSGCTSK GVDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE
610 620 630 640 650 660
VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE
670 680 690 700 710 720
YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA
730 740 750 760 770 780
VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ
KSLSLSPGK
M.W.: Calculated molecular mass (kDa): 86.4; Estimated by SDS-PAGE under reducing condition (kDa): 100-105 and 75-85
Calculated PI: 6.24
Calculated extinction coefficients (M-1 cm-1, at 280nm): 80215
Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Bioactivity: Binds to its ligands including TGFB1, TGFB3 and BMP9, and blocks the ligand-induced signaling activities.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.