Human CD105/Endoglin/ENG/END/HHT1 Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA
CD105 or Endoglin (ENG) is a single-pass type I transmembrane glycoprotein. It is a homodimer and a major glycoprotein of the vascular endothelium. CD105 is involved in the regulation of angiogenesis and plays a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 is a also component of the transforming growth factor beta receptor (TGFBR)complex and it binds to the beta1 and beta3 peptides with high affinity. CD105 acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. CD105 is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia (HHT1), also known as Osler-Rendu-Weber syndrome 1 (ORW1), an autosomal dominant multisystemic vascular dysplasia. The CD105 gene is also implicated in preeclampsia and several types of cancer.
Gene Symbol: CD105; Endoglin; ENG; END; HHT1; ORW1
NCBI Gene ID: 2022
Uniprot Entry: P17813
Construct Details: Full length human CD105 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.
Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)
Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)
Gene Insert Size: 1977 (bp)
Gene Insert Sequence:
ATGGACCGCGGCACGCTCCCTCTGGCTGTTGCCCTGCTGCTGGCCAGCTGCAGCCTCAGCCCCACAAGTC
TTGCAGAAACAGTCCATTGTGACCTTCAGCCTGTGGGCCCCGAGAGGGGCGAGGTGACATATACCACTAG
CCAGGTCTCGAAGGGCTGCGTGGCTCAGGCCCCCAATGCCATCCTTGAAGTCCATGTCCTCTTCCTGGAG
TTCCCAACGGGCCCGTCACAGCTGGAGCTGACTCTCCAGGCATCCAAGCAAAATGGCACCTGGCCCCGAG
AGGTGCTTCTGGTCCTCAGTGTAAACAGCAGTGTCTTCCTGCATCTCCAGGCCCTGGGAATCCCACTGCA
CTTGGCCTACAATTCCAGCCTGGTCACCTTCCAAGAGCCCCCGGGGGTCAACACCACAGAGCTGCCATCC
TTCCCCAAGACCCAGATCCTTGAGTGGGCAGCTGAGAGGGGCCCCATCACCTCTGCTGCTGAGCTGAATG
ACCCCCAGAGCATCCTCCTCCGACTGGGCCAAGCCCAGGGGTCACTGTCCTTCTGCATGCTGGAAGCCAG
CCAGGACATGGGCCGCACGCTCGAGTGGCGGCCGCGTACTCCAGCCTTGGTCCGGGGCTGCCACTTGGAA
GGCGTGGCCGGCCACAAGGAGGCGCACATCCTGAGGGTCCTGCCGGGCCACTCGGCCGGGCCCCGGACGG
TGACGGTGAAGGTGGAACTGAGCTGCGCACCCGGGGATCTCGATGCCGTCCTCATCCTGCAGGGTCCCCC
CTACGTGTCCTGGCTCATCGACGCCAACCACAACATGCAGATCTGGACCACTGGAGAATACTCCTTCAAG
ATCTTTCCAGAGAAAAACATTCGTGGCTTCAAGCTCCCAGACACACCTCAAGGCCTCCTGGGGGAGGCCC
GGATGCTCAATGCCAGCATTGTGGCATCCTTCGTGGAGCTACCGCTGGCCAGCATTGTCTCACTTCATGC
CTCCAGCTGCGGTGGTAGGCTGCAGACCTCACCCGCACCGATCCAGACCACTCCTCCCAAGGACACTTGT
AGCCCGGAGCTGCTCATGTCCTTGATCCAGACAAAGTGTGCCGACGACGCCATGACCCTGGTACTAAAGA
AAGAGCTTGTTGCGCATTTGAAGTGCACCATCACGGGCCTGACCTTCTGGGACCCCAGCTGTGAGGCAGA
GGACAGGGGTGACAAGTTTGTCTTGCGCAGTGCTTACTCCAGCTGTGGCATGCAGGTGTCAGCAAGTATG
ATCAGCAATGAGGCGGTGGTCAATATCCTGTCGAGCTCATCACCACAGCGGAAAAAGGTGCACTGCCTCA
ACATGGACAGCCTCTCTTTCCAGCTGGGCCTCTACCTCAGCCCACACTTCCTCCAGGCCTCCAACACCAT
CGAGCCGGGGCAGCAGAGCTTTGTGCAGGTCAGAGTGTCCCCATCCGTCTCCGAGTTCCTGCTCCAGTTA
GACAGCTGCCACCTGGACTTGGGGCCTGAGGGAGGCACCGTGGAACTCATCCAGGGCCGGGCGGCCAAGG
GCAACTGTGTGAGCCTGCTGTCCCCAAGCCCCGAGGGTGACCCGCGCTTCAGCTTCCTCCTCCACTTCTA
CACAGTACCCATACCCAAAACCGGCACCCTCAGCTGCACGGTAGCCCTGCGTCCCAAGACCGGGTCTCAA
GACCAGGAAGTCCATAGGACTGTCTTCATGCGCTTGAACATCATCAGCCCTGACCTGTCTGGTTGCACAA
GCAAAGGCCTCGTCCTGCCCGCCGTGCTGGGCATCACCTTTGGTGCCTTCCTCATCGGGGCCCTGCTCAC
TGCTGCACTCTGGTACATCTACTCGCACACGCGTTCCCCCAGCAAGCGGGAGCCCGTGGTGGCGGTGGCT
GCCCCGGCCTCCTCGGAGAGCAGCAGCACCAACCACAGCATCGGGAGCACCCAGAGCACCCCCTGCTCCA
CCAGCAGCATGGCATAG
Primers: Gene insert coding sequence can be confirmed by following primers:
(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;
(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’
Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.