Human LILRB5/CD85c/LIR8 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

LILRB5 (Leukocyte immunoglobulin like receptor B5), also known as CD85c, and LIR8 (Leukocyte immunoglobulin-like receptor 8),  is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  It belongs to the subfamily B class of LIR receptors (LILRBs), which are single-pass type I trnasmembrane proteins with 2 or 4 extracellular Ig-like domains, a transmembrane domain (TM), and 2-4 cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB5 has 4 Ig-like C2 type domains and 2 TIIMs. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases to modulate cellular responses.  LILRB5 is expressed predominantly on NK cells.  Like LILRB1 & LILRB, LILRB5 binds to MHC class I molecules on antigen-presenting cells (APCs) and transduces a negative signal that inhibits stimulation of an immune response. LILRB3 may control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. 

 

 

Gene Symbol: LILRB5; CD85c;  LIR-8; LIR8

 

NCBI Gene ID: 10990

 

Uniprot Entry: O75023

 

Construct Details: Full length human LILRB5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter and with or without a marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1773 (bp)

 

Gene Insert Sequence:  

ATGACCCTCACCCTCTCAGTCCTGATTTGCCTCGGGCTGAGTGTGGGCCCCAGGACCTGCGTGCAGGCAG

GCACCCTCCCCAAACCCACCCTCTGGGCTGAGCCAGCCTCTGTGATAGCTCGGGGGAAGCCCGTGACCCT

CTGGTGTCAGGGGCCCCTGGAGACTGAGGAGTACCGTCTGGATAAGGAGGGACTCCCATGGGCCCGGAAG

AGACAGAACCCACTGGAGCCTGGAGCCAAGGCCAAGTTCCACATTCCATCCACGGTGTATGACAGTGCAG

GGCGATACCGCTGCTACTATGAGACCCCTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAGCTGGTGGC

GACAGGATTCTATGCAGAACCCACTCTTTTAGCCCTGCCGAGTCCTGTGGTGGCCTCAGGAGGAAATGTG

ACCCTCCAGTGTGATACACTGGACGGACTTCTCACGTTTGTTCTTGTTGAGGAAGAACAGAAGCTCCCCA

GGACCCTGTACTCACAGAAGCTCCCCAAAGGGCCATCCCAGGCCCTGTTCCCTGTGGGTCCCGTGACCCC

CAGCTGCAGGTGGAGGTTCAGATGCTATTACTATTACAGGAAAAACCCTCAGGTGTGGTCGAACCCCAGT

GACCTCCTGGAGATTCTGGTCCCAGGCGTGTCTAGGAAGCCCTCCCTCCTGATCCCGCAGGGCTCTGTCG

TGGCCCGCGGAGGCAGCCTGACCCTGCAGTGTCGCTCTGATGTCGGCTATGACATATTCGTTCTGTACAA

GGAGGGGGAACATGACCTCGTCCAGGGCTCTGGCCAGCAGCCCCAGGCTGGGCTCTCCCAGGCCAACTTC

ACCCTGGGCCCTGTGAGCCGCTCCCACGGGGGCCAGTACAGATGCTACGGTGCACACAACCTCTCCCCTA

GGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGACTGATCCCTGACATACCCGCCCTCTC

GGTGCAGCCGGGCCCCAAGGTGGCCTCAGGAGAGAACGTGACCCTGCTGTGTCAGTCATGGCATCAGATA

GACACTTTCTTTTTGACCAAGGAGGGGGCAGCCCATCCCCCGCTGTGTCTAAAGTCAAAGTACCAGTCTT

ATAGACACCAGGCTGAATTCTCCATGAGTCCTGTGACCTCAGCCCAGGGTGGAACCTACCGATGCTACAG

CGCAATCAGGTCCTACCCCTACCTGCTGTCCAGCCCTAGTTACCCCCAGGAGCTCGTGGTCTCAGGACCC

TCTGGGGATCCCAGCCTCTCACCTACAGGCTCCACCCCCACACCTGGCCCTGAGGACCAGCCCCTCACCC

CCACGGGGTTGGATCCCCAGAGTGGTCTGGGAAGGCACCTGGGGGTTGTGACTGGGGTCTCAGTGGCCTT

CGTCCTGCTGCTGTTCCTCCTCCTCTTCCTCCTCCTCCGACATCGGCATCAGAGCAAACACAGGACATCG

GCCCATTTCTACCGTCCTGCAGGGGCTGCGGGGCCAGAGCCCAAGGACCAGGGCCTGCAGAAGAGGGCCA

GCCCAGTTGCTGACATCCAGGAGGAAATTCTCAATGCTGCCGTGAAGGACACACAGCCCAAGGACGGGGT

GGAGATGGATGCTCCGGCTGCTGCATCTGAAGCCCCCCAGGATGTGACCTACGCCCAGCTGCACAGCTTG

ACCCTCAGACGGGAGGCAACTGAGCCTCCTCCATCCCAGGAAAGGGAACCTCCAGCTGAACCCAGCATCT

ACGCCCCCCTGGCCATCCACTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

1. J. Exp. Med. 186:1809-1818(1997)

2. J. Immunol. 159:5192-5196(1997)

3. Eur. J. Immunol. 28:3423-3434(1998)

4. Immunity 11:603-613(1999) 

5. J. Immunol. 168:3351-3359(2002)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.