Human GPR179 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

GPR179 (Probable G-protein coupled receptor 179) is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length. Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors. These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. GPR179 is an orphan receptor whose function remains unknown. It may be involved in vision for signal transduction through retinal depolarizing bipolar cells. GPR179 has an EGF-like calcium binding domain and a 7-TM domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E.

 

 

Gene Symbol: GPR179; CSNB1E; GPR158L, GPR158L1

 

NCBI Gene ID: 440435

 

Uniprot Entry: Q6PRD1

 

Construct Details: Full length human GPR179 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 7104 (bp)

 

Gene Insert Sequence:  

ATGGGCACCAGGGGAGCGGTCATGCCCCCTCCTATGTGGGGGCTGCTGGGCTGCTGTTTTGTCTGTGCCT

GGGCTCTGGGGGGTCCACGGCCCATCCGCTCTCTGCCCCCTCTGTCTTCCCAAGTCAAGCCAGGATCTGT

ACCCATGCAGGTGCCCCTAGAGGGGGCCGAGGCCGCCCTCGCTTATCTCTACTCTGGAGATGCCCAGCAG

CTATCACAGGTGAATTGCAGTGAGCGCTATGAAGCGCGTGGGGCAGGAGCCATGCCAGGGCTCCCCCCAA

GCCTACAGGGGGCAGCGGGCACCCTTGCCCAGGCCGCCAATTTTCTCAACATGCTGCTGCAAGCCAACGA

CATCCGTGAGTCCAGTGTGGAGGAGGATGTGGAATGGTACCAGGCACTGGTCCGCAGCGTGGCCGAGGGG

GACCCAAGAGTGTACAGGGCTTTGCTGACCTTTAACCCTCCACCAGGGGCCAGCCACCTACAGCTGGCCC

TGCAGGCCACCCGGACTGGGGAGGAAACCATCCTGCAGGACTTGTCTGGGAACTGGGTGCAGGAGGAGAA

CCCTCCTGGGGACCTGGACACCCCTGCCCTGAAGAAGCGAGTGTTGACCAATGACCTAGGGAGCCTCGGC

AGCCCCAAGTGGCCGCAGGCAGATGGATATGTGGGGGACACGCAGCAGGTGAGGCTGTCTCCTCCTTTCC

TGGAATGCCAGGAGGGACGGCTCCGACCTGGATGGCTGATCACACTCTCTGCCACCTTCTATGGACTCAA

GCCAGACCTCAGCCCAGAAGTCAGGGGGCAGGTGCAGATGGACGTAGATCTCCAGAGTGTGGACATCAAT

CAGTGTGCAAGTGGCCCAGGCTGGTACTCTAACACACACCTGTGTGATCTCAACAGCACCCAGTGTGTCC

CCCTGGAGAGTCAGGGCTTTGTTCTTGGCCGCTACCTCTGCCGCTGCCGACCTGGATTCTACGGGGCAAG

CCCCTCTGGGGGGTTAGAGGAGAGTGACTTCCAGACTACCGGGCAATTCGGGTTCCCAGAAGGCAGATCT

GGGAGACTGCTGCAGTGTCTGCCATGTCCTGAGGGCTGCACCAGCTGCATGGATGCCACACCGTGCCTGG

TGGAAGAGGCCGCGGTGCTGCGGGCCGCTGTGCTGGCCTGCCAGGCCTGCTGCATGCTGGCCATCTTCCT

GAGCATGCTGGTCTCCTACCGCTGCCGCCGGAACAAGAGGATCTGGGCATCTGGAGTGGTCCTGCTGGAA

ACTGTCCTTTTTGGATTCCTGCTGCTTTACTTTCCTGTCTTCATCCTATACTTCAAGCCCAGTGTATTCC

GCTGCATCGCTCTTCGCTGGGTGCGGCTGCTGGGTTTTGCCATCGTCTACGGCACCATCATACTCAAGCT

TTACAGAGTGCTGCAGCTGTTTCTGTCTCGAACGGCCCAGCGGAGTGCCCTTCTGAGCAGCGGGCGGCTG

CTGCGGCACCTGGGGCTGCTCCTGCTACCTGTGCTGGGCTTCCTGGCTGTGTGGACCGTGGGCGCCCTGG

AGCGAGGCATCCAGCACGCACCTCTGGTGATCCGAGGCCACACTCCCAGTGGCCGCCATTTCTACCTCTG

TCACCACGACCGCTGGGACTACATCATGGTTGTGGCTGAGCTGCTGCTGCTGTGCTGGGGCAGCTTCCTC

TGCTACGCCACACGGGCTGTGCTCTCGGCCTTCCATGAGCCACGCTACATGGGCATCGCCCTGCACAATG

AGCTACTGCTTTCCGCTGCCTTCCACACAGCCAGGTTTGTGCTGGTTCCCTCTCTGCACCCGGACTGGAC

CCTCCTCCTCTTCTTCTTCCACACCCACAGCACAGTCACCACCACGCTGGCTCTGATCTTCATCCCTAAG

TTCTGGAAGCTGGGGGCTCCTCCCCGGGAGGAGATGGTGGATGAGGTGTGTGAGGACGAGCTGGACCTGC

AGCACTCAGGCTCCTACCTTGGCAGCAGCATCGCCTCAGCCTGGAGTGAGCACAGCCTGGACCCTGGAGA

CATTCGGGACGAGCTGAAGAAGCTCTATGCCCAGCTAGAGGTCCACAAAACCAAGGAAATGGCCGCAAAC

AACCCCCACCTGCCCAAGAAGCGAGGCAGCTCATGCCAGGGACTGGGCCGCTCCTTCATGAGGTACCTGG

CGGAATTCCCCGAGGCCCTGGCCAGGCAGCACTCCCGGGACTCAGGATCCCCAGGCCACGGCAGCCTGCC

CGGCTCCTCCCGCCGCCGGCTCCTCAGCTCCAGCCTCCAGGAACCCGAGGGGACACCAGCTCTGCACAAG

TCCCGCAGCACCTATGACCAGCGCAGGGAGCAGGACCCGCCTCTTCTTGACTCACTGCTGAGGAGGAAGC

TGGCCAAGAAGGCCTCTCGAACAGAGAGCCGGGAGTCGGTGGAGGGGCCCCCTGCCCTGGGCTTCAGGTC

AGCCAGCGCCCACAACCTGACGGTGGGAGAGAGGCTACCCAGAGCCCGGCCCGCCTCTCTGCAGAAGTCG

CTCAGTGTGGCCAGCTCCAGGGAAAAGGCCTTGCTCATGGCCAGCCAGGCCTACCTGGAGGAGACCTACC

GGCAAGCAAAGGAGCGGGAGGAGCGGAAGAAGGCCAAGGCAGCCATGGCCAGCCTGGTGCGGAGGCCATC

AGCCAGGAGGCTGGAGCGGCCTCGAGGGGCCCCCCTGTCAGCTCCACCTTCCCCTGCCAAGAGCAGCAGC

GTGGACAGCTCTCACACCTCTGGGAGGCTTCATGAGGAGGCTAGGAGAAGGCTGCCTCATCCACCCATCA

GGCACCAGGTTTCTACCCCCATCTTGGCCCTGTCTGGGGGCCTGGGAGAGCCAAGGATGCTATCTCCCAC

CTCCACCTTGGCTCCAGCTCTGCTGCCAGCTCTAGCTCCAACCCCAGCCCCTGCCCTGGCACCAGTCCCA

GTATCCCCACAAAGCCCCAACTTACTCACCTACATCTGCCCCTGGGAGAACGCAGAACTGCCAGCCAAGC

AAGAAAATGTGCCCCAGGAAGGCCCCTCAGGGCCAGAGCGAGGCCACCACTCCCCTGCCCCAGCTCGAGC

CAGGCTCTGGAGGGCCCTCTCTGTTGCAGTAGAGAAAAGCAGGGCTGGGGAGAATGAGATGGACGCAGAG

GATGCACATCACCAGAGGGAAGCTAATGATGTGGACGAAGACAGGCCCAAGATCTTCCCTAAATCCCACA

GCCTCAAGGCCCCTGTTCAGCAGGGTTCCATGCGCAGCCTGGGGCTGGCGATTAAAGCTCTGACCCGTTC

TCGGAGCACCTACAGAGAGAAGGAGAGTGTGGAGGAGAGTCCCGAGGGGCAGAACAGCGGGACTGCGGGA

GAGAGTATGGGGGCACCCTCCCGATCGCCCAGGCTAGGCCGGCCCAAGGCGGTGAGTAAGCAGGCCGCTC

TTATCCCCTCCGATGACAAGGAGTCCCTCCAGAACCAACAGAACGCTCACACCAGCAGGATGCTCCAAGT

CTGTCAACGGGAGGGCAGCAGGGAACAAGAAGACAGAGGCAGGAGGATGACCCAGGGTCTAGGGGAACGG

AAAGCTGAGAGAGCAGGTAAAACAGGGCTTGCCATGCTGAGGCAAGTTTCCAGGGACAAAAACATCAAGC

AATCAAAAGAAACCCCTGTCGGGTGGCAGGAACTGCCCAAAGCTGGCCTCCAGTCCCTGGGCAGCGCTGA

CCACAGGGTGGCAGAGGTATGCCCCTGGGAGGTCACTGAATCAGAAACGCGTCAGCCAGACAGTGGCAAC

AAGGCCGAAATCTGCCCCTGGGAGACGAGTGAAGGAGCCCCAGAGTCGAGGGCACTAAGACAAGACCCAG

GTGACTCCCAAAAAAAGAGAGGGGAGGCCCGGGGAAAATCAGAGCCCATAGATGTGGTTCCCATGATGCG

GAAAAAGCCAGAGAGGCTGGTGAGGGAGCAGGAAGCAGTGTGTCCCTGGGAGAGTGCCGATCGAGGAGGT

CTGTCCCCTGGGTCAGCTCCTCAGGACCCTGGCAGAATCAGAGACAAATCTGAGGCGGGGGACAGTGTGG

AGGCCAGGAAGGTGGAGAAGCCTGGGTGGGAAGCTGCTGGCCCAGAAGCTCATACCCCTGACATCACCAA

GGCAGAGCCGTGTCCCTGGGAGGCAAGTGAAGGAGGCGAGGATGGGAAACCAGCCCAAGAGGCAGTGAAG

GATCTCCCTCAGGAAAAGCAGAAAACCAGGAAAGCAACCTTTTGGAAAGAACAGAAACCGGGAGGAGACT

TGGAGTCTCTTTGTCCATGGGAGAGTACAGATTTCCGGGGCCCCTCAGCAGTCTCAATTCAGGCCCCAGG

AAGCTCAGAGTGTTCAGGGAGTTTGGGCAGTGGCATTGCTGAAGTGTGTCTGTGGGAGGCAGGAGATGCT

CCTGCTATCCAGAAAGCAGAGATCTGTCCCTGGGAGCTGGATGATAACGTGATGGGGCAGGAAATGCTGA

GTCTGGGGACAGGTAGAGAATCTCTTCAAGAAAAGGAAAAAGCCTCCAGAAAAGGAAGCTTTGGAGAGAT

GGGGGAACAAACTGTGAAAGCAGTGCAGAAATTAAGTCAACAGCAGGAGTCAGTTTGTCCCAGGGAGAGC

ACGGTCCCTGGGCACTCCAGCCCATGTCTAGACAATTCCTCATCCAAAGCTGGTAGCCAATTCCTATGCA

ATGGAGGAAGCAGAGCAACGCAGGTGTGTCCACAGGAAGATCTCAGGCCGGAGGCACAGGAAGCAACACC

TGCCAAAACAGAAATCTGTCCCTGGGAGGTAAATGAAAGAACAAGAGAGGAATGGACATCAGCACAGGTG

CCAAGAGGAGGAGAATCTCAAAAGGACAAGGAGAAAATGCCTGGAAAATCGGAAATCGAAGATGTCACAG

CTTGGGAAAAGCCTGAGGGGCAGATCCAAAAGCAAGAAGCGGTCGGCCCCTGGGAGAGTGTGGACCCTGG

CAGCTTCTCCCCACAACCACGTCCTCAAGACACAGAGAGACCCCAAACCCTTCTCCAGATGTCAGGCAGT

GTGGGAAGCAAAGCTGCCGACATTTGCCCTTTGGATGTGGAGGAAAACTTGACTGCTGGGAAGGCAGAAA

TCTGTCCCTGGGAGGTGGGTGCTGGAGCAGGGGAGGAAAGGGCTTTGGGAGCTGAGGCCATTAGGAAATC

TCCAAATGATACAGGCAAGGTTTCTGCAGATCTTGGACCCAGGGAGAGAGCTGTTACTGCTCCAGAGAAG

CCACAGAAGCCAACCCCAGAGTGGGAGGTGGCTTGTCCCTGGGGGAGTGTGGGTCCAGGGGCCTGTTCTC

AGCATCCAGGTACTCTAGATGCTGATGGACCAAAAGCTGGGTTCCAGGAACTGGATCATATGGGCTGCAG

GCCAGGTGAAGTGTGTCCCTGGGAAGCACAGGAAGCTGCTACCAGTGAAAAAGCCAAGATCTGTCCCTGG

GAGGTAAGTGAAGGAACTACTGGGAAGGGATTGGACCAAAAGGCAGGGAGTGAATCAGCAGAGCAGAGGG

AGAAAGCTCTAGAAAAGGGGAGACTCACTTCCCTGGGAGAAGACGTATCAAAAGGGATGGCAAAACTGTG

TCAACAACAGGAAACTATTTGTATTTGGGAGAACAAGGACTTGAGGGAATCCCCTGCTCAGGCCCCCAAG

ATCTCAGACTTGCCCAGCAGCATGAGTAGTGAAGTGGCAGAGGGACATTCCTTGGAAGCAACAGAGAAGG

GGGACCTGAGACAAGACCCAAAGACAGGTTCCTTCCCAGAACACATAACCCAAGAAAAAGCTCCAGCTGC

AGACACAGAAGAATTCACTACTGAAGATGGGGAAAAAACAAGCCATGAGCTACAATCCGTCTGTCCATGG

GAGACCACTGCCCCAGCAGATTCCGTCTCTCACCTAGACAGACAGCGCCCTGACCAACCTAAAGCTAGCT

CCCAGAGACTGGTCAGCACTGGGGGCAGGGCCGCTGACGTGTGCCCATGGGATGTTCCTGATGCAGGTGT

GTATAAATCTGACAGCAGTGCCAAGGCTGAGACCTGTCCCTGGGAAGTGACTGAAAGAATCCCTGTCAAA

GGGGTGTCAAGGCAGGATGGAAAAGGGGACTCTCAAGAAGAGAAAGGCAGAGCCCCAGAAAAATCAGAGC

CAAAAGGTGTGCCAGTTCAGAAAAAGCCAGAGATGGCAGACTTCAGGCAGCAGGAGGCTGTGTGTCCCTG

GGAGAGTCAAGATGGCAAGGGTCTGTCCCCACAGCCAGCCCCAGATGCTTCTGACAGAAGCAGAGGCAGT

TCTGAGGCAGCAGGCAGTGTGGAGACCAGGGTAGCGGAAGTGTGTCTGTGGGAAGTGGTAGAGGCTCCCT

CTGCCAAGAAAGCAGAGATCTGCCCTTGGGAGGCGGGTGGAGGAGCAGCAGAGGAAGGGGAACAGGAAAG

AGAATCACAAGGGCAAGGAGAGATGTTCCTTCAGAAGGCAGGACCTGGAGGGACGGAAGAACACTTCTCA

AAAGCAGCAGCAAAGCCCAGAGAGCAGGAGGCAGTCTGCCCTGGGGAAGGCACAGGCTCAGGAGGGCTCT

TGCCCCAGTCAGGTGCCCTGGACCCAGAACTCAAAGTCAGCCCCAAGGAAGCAGGCAGCATGGGAAGCAG

GATGGCAGAGCTGTGCCAATGGGAAATCACAGATCCAGAAGGAAATAAAATAAAGGGTACCATGGCAGAC

ATCTGTCCTGGGGAGGAAACTGGAGTCCCATCTGAGGAATCTGGCCTCCTGGCTTTAACAGCAACTCGGA

GAGAATTTTTCCCCACAGCTCCTGAAAAACCACTATGCCTTTTAGTCCATGGGCCTCTGGATCACTTCTT

TCCAGAAAGCAAAATCCCCTGCCCCAAGGTAAGCAGGCCAGCCAGTACTTTCACTCTAGAAGGTGTCAGA

GAACTACAAGGACCTTCAGGGCTTGAGCCAAGGACCAGCTTAGCCCCAGAGCCAAGTCTCCAGGAAGCTG

AGTCTCAGTCTTCGTCCTTAACTGAAGACTCAGGCCAAGTGGCTTTTGAAGCTCAGTATGAAGAATTCAC

CCCTCCCACTGTCTATCCTTGGGATTGGGAGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’