Human GPR158 Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

GPR158 (Probable G-protein coupled receptor 158) is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length. Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors.  These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. GPR158 is an orphan receptor whose function remains unknown.

 

 

Gene Symbol: GPR158; KIAA1136

 

NCBI Gene ID: 57512

 

Uniprot Entry: Q5T848

 

Construct Details: Full length human GPR158 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3648 (bp)

 

Gene Insert Sequence:  

ATGGGAGCCATGGCTTACCCCTTACTCCTCTGCCTCCTGCTTGCTCAGCTGGGATTGGGAGCTGTTGGCG

CCAGCCGCGACCCCCAAGGACGGCCGGATTCCCCTCGAGAGAGGACCCCGAAGGGGAAGCCGCACGCCCA

GCAGCCGGGTCGAGCCTCTGCCTCGGACTCCTCGGCTCCCTGGAGCCGCTCCACCGATGGCACCATCTTG

GCGCAGAAACTCGCCGAGGAGGTGCCCATGGACGTGGCCTCTTACCTCTACACCGGGGACTCCCACCAGC

TGAAGCGAGCCAACTGCTCCGGCCGCTACGAGTTGGCGGGCCTGCCGGGGAAGTGGCCAGCCCTGGCCAG

CGCGCACCCCTCCTTGCACCGGGCGCTGGACACACTGACACACGCCACCAACTTCCTCAACGTGATGCTG

CAGAGCAATAAGTCGCGGGAGCAGAACTTGCAGGACGACCTGGATTGGTACCAGGCGCTGGTGTGGAGCC

TTCTGGAGGGCGAGCCCAGCATCTCCCGGGCGGCCATCACCTTCAGCACCGATTCGCTGTCCGCACCGGC

CCCACAGGTCTTCCTCCAGGCCACGCGCGAGGAGAGCCGCATCCTGCTCCAAGACCTGTCCTCCTCCGCA

CCCCACCTGGCCAACGCCACTCTGGAGACCGAGTGGTTCCACGGCCTCCGGCGCAAGTGGAGGCCCCACT

TACACCGCCGCGGCCCCAATCAGGGGCCCCGGGGCCTGGGCCACAGCTGGCGGCGCAAGGACGGGCTCGG

CGGGGACAAGAGCCACTTCAAGTGGTCTCCGCCTTATCTGGAGTGCGAGAACGGGAGTTACAAGCCCGGG

TGGCTGGTTACTCTTTCCTCTGCCATCTACGGGTTGCAGCCTAACCTGGTCCCGGAATTCAGGGGTGTCA

TGAAAGTTGACATAAATCTTCAGAAAGTGGACATTGACCAATGCTCAAGTGATGGCTGGTTTTCAGGAAC

TCATAAATGCCACCTCAACAATTCAGAGTGTATGCCAATTAAAGGCCTAGGATTCGTTCTTGGAGCCTAT

GAGTGCATTTGCAAAGCAGGATTCTATCATCCTGGAGTCTTACCAGTGAACAACTTTCGGAGAAGGGGTC

CGGATCAGCATATTTCAGGAAGTACAAAAGATGTGTCAGAAGAAGCCTATGTCTGCCTACCTTGCAGGGA

GGGCTGCCCCTTCTGTGCTGATGACAGCCCATGCTTCGTCCAGGAAGATAAGTATTTACGACTTGCCATC

ATCTCCTTCCAAGCCCTGTGTATGCTGCTCGACTTCGTTAGCATGCTGGTGGTCTACCACTTTCGCAAAG

CAAAGAGCATCCGGGCATCGGGCCTTATCCTGTTGGAAACGATCCTTTTTGGATCTCTGCTCCTATACTT

TCCAGTTGTTATTTTGTACTTTGAGCCAAGCACATTTCGCTGTATTCTCCTAAGATGGGCTCGTCTTCTC

GGTTTTGCTACTGTTTACGGAACTGTCACTCTCAAACTTCACAGGGTTTTGAAGGTGTTTCTTTCACGAA

CGGCTCAACGAATTCCATATATGACTGGCGGACGGGTCATGAGGATGCTGGCAGTAATACTCTTGGTAGT

GTTTTGGTTTCTCATTGGCTGGACTTCATCTGTGTGCCAGAATTTGGAGAAACAGATTTCACTTATTGGC

CAGGGGAAAACATCCGATCACCTCATCTTCAATATGTGCCTCATTGACCGCTGGGACTACATGACAGCAG

TTGCTGAATTTTTATTCCTCTTGTGGGGTGTTTATCTCTGCTATGCAGTGCGGACAGTCCCATCGGCATT

CCATGAGCCCCGCTATATGGCTGTTGCAGTTCACAATGAGCTCATCATCTCTGCTATATTCCATACAATT

AGATTTGTTCTTGCCTCAAGACTTCAGTCTGATTGGATGTTGATGCTGTATTTTGCACATACTCATTTGA

CTGTGACAGTCACCATTGGGTTGCTTTTGATTCCAAAGTTTTCACATTCAAGCAATAACCCACGAGATGA

TATTGCTACAGAAGCATATGAGGATGAGCTAGACATGGGCCGATCTGGATCCTACCTGAACAGCAGTATC

AATTCAGCCTGGAGTGAGCACAGCTTGGATCCAGAGGACATTCGGGACGAGCTGAAAAAACTCTATGCCC

AACTGGAAATATATAAAAGAAAGAAGATGATCACAAACAACCCCCACCTCCAGAAAAAGCGGTGCTCGAA

GAAGGGCCTAGGTCGTTCCATCATGAGACGCATTACGGAGATCCCAGAGACAGTCAGCCGGCAGTGCTCT

AAAGAGGACAAGGAGGGCGCCGACCATGGCACAGCCAAAGGCACTGCCCTCATCAGGAAGAACCCCCCAG

AGTCTTCAGGGAACACAGGGAAATCCAAGGAGGAGACCCTGAAAAACCGAGTCTTCTCACTCAAGAAATC

CCACAGCACTTATGACCACGTGAGAGACCAAACGGAAGAGTCCAGTAGCCTACCCACAGAAAGCCAAGAG

GAGGAGACAACAGAAAATTCCACACTGGAATCCCTGTCGGGTAAAAAACTAACACAAAAACTAAAAGAAG

ACAGCGAGGCTGAGTCCACGGAGTCGGTGCCGTTGGTGTGCAAGTCAGCAAGCGCTCACAACCTCAGCTC

AGAGAAGAAAACTGGGCACCCACGAACATCGATGTTACAGAAGTCTCTCAGTGTCATAGCAAGCGCCAAG

GAGAAGACTCTTGGATTAGCTGGGAAAACCCAAACAGCAGGTGTGGAAGAACGCACTAAATCCCAGAAAC

CTTTGCCAAAAGATAAAGAGACAAACAGAAATCACTCAAATTCTGATAACACAGAGACTAAAGATCCTGC

CCCCCAAAACTCAAATCCTGCGGAGGAGCCAAGAAAGCCTCAGAAATCTGGGATTATGAAACAACAAAGG

GTCAACCCCACCACTGCCAATTCTGACCTGAACCCAGGCACCACCCAGATGAAGGACAACTTTGACATTG

GGGAGGTGTGTCCTTGGGAGGTTTATGACCTGACCCCTGGTCCTGTGCCTTCAGAATCAAAAGTTCAAAA

GCACGTATCTATTGTGGCTTCTGAAATGGAGAAAAACCCCACTTTTTCCTTAAAGGAGAAATCTCACCAC

AAGCCTAAGGCAGCTGAGGTTTGTCAGCAATCCAATCAGAAGCGCATAGATAAGGCTGAAGTATGCCTTT

GGGAGAGCCAAGGCCAGTCCATTTTGGAAGATGAGAAGCTTTTGATTTCCAAGACTCCAGTTCTCCCAGA

GAGGGCAAAAGAGGAGAACGGAGGTCAGCCTCGTGCAGCCAATGTGTGTGCTGGGCAGAGCGAAGAACTG

CCCCCCAAAGCTGTAGCATCAAAAACAGAGAATGAAAATCTCAACCAAATAGGACACCAGGAAAAAAAGA

CATCTTCTTCTGAGGAGAATGTGCGTGGCTCCTATAACTCAAGTAATAACTTCCAGCAACCTTTAACATC

ACGAGCAGAGGTTTGTCCTTGGGAGTTTGAGACCCCAGCTCAACCAAATGCTGGAAGAAGTGTAGCTTTA

CCTGCCTCTTCTGCTCTAAGTGCAAATAAGATAGCAGGGCCTAGGAAAGAAGAGATCTGGGATAGTTTTA

AAGTGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.