Human GPRC6A Gene, Full-length cDNA Clone in Lentivector, Endotoxin-free DNA

Description

GPRC6A (G-protein coupled receptor family C group 6 member A) is a 7 transmembrane (7TM) receptor belonging to the family 3 (also known as family C) of the G-protein-coupled receptors (GPCRs). Structurally they are composed of 4 elements; an N-terminal signal peptide; a large hydrophilic extracellular agonist-binding region; a region containing 7TM domains; and a C-terminal cytoplasmic domain of variable length. Family 3 members include the metabotropic glutamate receptors (mGluRs), the extracellular calcium-sensing receptors (CASRs), the gamma-amino-butyric acid (GABA) type B receptors, and the vomeronasal type-2 receptors.  These GPCRs regulate many important physiological processes and many of them are promising targets for drug development. GPRC6A may be a receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. It can be activated by extracellular calcium and osteocalin. GPRC6A may also mediate the non-genomic effects of androgens in multiple tissue. It may coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.

 

 

Gene Symbol: GPRC6A

 

NCBI Gene ID: 222545

 

Uniprot Entry: Q5T6X5

 

Construct Details: Full length human GPRC6A gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2781 (bp)

 

Gene Insert Sequence:  

ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACCC

CTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAAAA

AATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCAGTT

TTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTACCTGGAGTCA

AACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGGTTTCT

TTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCAAGAGTT

AAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTACAGCTCA

TGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCATTTTTACG

GACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGTTGGAACTGG

ATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATTCAGGCTGAAG

CAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACCATTGAAGTCAG

AATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGGTTAATGTCATTGTGGTATTTCTGAGGCAA

TTCCATGTTTTTGATCTCTTCAATAAAGCCATTGAAATGAATATAAATAAGATGTGGATTGCTAGTGATA

ATTGGTCAACTGCCACCAAGATTACCACCATTCCTAATGTTAAAAAGATTGGCAAAGTTGTAGGGTTTGC

CTTTAGAAGAGGGAATATATCCTCTTTCCATTCCTTTCTTCAAAATCTGCACTTGCTTCCCAGTGACAGT

CACAAACTCTTACATGAATATGCCATGCATTTATCTGCCTGCGCATATGTCAAGGACACTGATTTGAGTC

AATGCATATTCAATCATTCTCAAAGGACTTTGGCCTACAAGGCTAACAAGGCTATAGAAAGGAACTTCGT

CATGAGAAATGACTTCCTCTGGGACTATGCTGAGCCAGGACTCATTCATAGTATTCAGCTTGCAGTGTTT

GCCCTTGGTTATGCCATTCGGGATCTGTGTCAAGCTCGTGACTGTCAGAACCCCAACGCCTTTCAACCAT

GGGAGTTACTTGGTGTGCTAAAAAATGTGACATTCACTGATGGATGGAATTCATTTCATTTTGATGCTCA

CGGGGATTTAAATACTGGATATGATGTTGTGCTCTGGAAGGAGATCAATGGACACATGACTGTCACTAAG

ATGGCAGAATATGACCTACAGAATGATGTCTTCATCATCCCAGATCAGGAAACAAAAAATGAGTTCAGGA

ATCTTAAGCAAATTCAATCTAAATGCTCCAAGGAATGCAGTCCTGGGCAAATGAAGAAAACTACAAGAAG

TCAACACATCTGTTGCTATGAATGTCAGAACTGTCCTGAAAATCATTACACTAATCAGACAGATATGCCT

CACTGCCTTTTATGCAACAACAAAACTCACTGGGCCCCTGTTAGGAGCACTATGTGCTTTGAAAAGGAAG

TGGAATATCTCAACTGGAATGACTCCTTGGCCATCCTACTCCTGATTCTCTCCCTACTGGGAATCATATT

TGTTCTGGTTGTTGGCATAATATTTACAAGAAACCTGAACACACCTGTTGTGAAATCATCCGGGGGATTA

AGAGTCTGCTATGTGATCCTTCTCTGTCATTTCCTCAATTTTGCCAGCACGAGCTTTTTCATTGGAGAAC

CACAAGACTTCACATGTAAAACCAGGCAGACAATGTTTGGAGTGAGCTTTACTCTTTGCATCTCCTGCAT

TTTGACGAAGTCTCTGAAAATTTTGCTAGCCTTCAGCTTTGATCCCAAATTACAGAAATTTCTGAAGTGC

CTCTATAGACCGATCCTTATTATCTTCACTTGCACGGGCATCCAGGTTGTCATTTGCACACTCTGGCTAA

TCTTTGCAGCACCTACTGTAGAGGTGAATGTCTCCTTGCCCAGAGTCATCATCCTGGAGTGTGAGGAGGG

ATCCATACTTGCATTTGGCACCATGCTGGGCTACATTGCCATCCTGGCCTTCATTTGCTTCATATTTGCT

TTCAAAGGCAAATATGAGAATTACAATGAAGCCAAATTCATTACATTTGGCATGCTCATTTACTTCATAG

CTTGGATCACATTCATCCCTATCTATGCTACCACATTTGGCAAATATGTACCAGCTGTGGAGATTATTGT

CATATTAATATCTAACTATGGAATCCTGTATTGCACATTCATCCCCAAATGCTATGTTATTATTTGTAAG

CAAGAGATTAACACAAAGTCTGCCTTTCTCAAGATGATCTACAGTTATTCTTCCCATAGTGTGAGCAGCA

TTGCCCTGAGTCCTGCTTCACTGGACTCCATGAGCGGCAATGTCACAATGACCAATCCCAGCTCTAGTGG

CAAGTCTGCAACCTGGCAGAAAAGCAAAGATCTTCAGGCACAAGCATTTGCACACATATGCAGGGAAAAT

GCCACAAGTGTATCTAAAACTTTGCCTCGAAAAAGAATGTCAAGTATATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.