Human CD26/DPP4/DPPIV/ADCP2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
CD26, also known as DPPIV/DPP4 (dipeptidyl peptidase-4) and ADCP2 (adenosine deaminase complexing protein 2), is a single pass type II transmembrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (ECD). The ECD of CD26 is a serine exopeptidase belonging to the S9B family that cleaves Xaa-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones such as glucagon. CD26 plays an important role in many biological and pathological processes. CD2 acts as a T cell-activating molecule and serves as a cofactor for entry of HIV in CD4+ cells. CD26 binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. CD26 exists as a homodimer on the cell surface and the soluble form is also detectable in human serum and other body fluids. The levels of soluble CD26 may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Therefore DPP4 inhibitors represent a new pharmacological class of drugs for treating Type 2 diabetes.
Gene Symbol: CD26; DPP4; ADABP; ADCP2; DPPIV; TP103
NCBI Gene ID: 1803
Uniprot Entry: P27487
Construct Details: The recombinant human CD26-Fc fusion is expressed as a 965 amino acid protein consisting of Asn29 - Pro766 region of CD26 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source: Human cells stably expressing human CD26-Fc and growing in chemical-defined media with no animal components or antibiotics
Deduced Amino Acid Sequence:
10 20 30 40 50 60
NKGTDDATAD SRKTYTLTDY LKNTYRLKLY SLRWISDHEY LYKQENNILV FNAEYGNSSV
70 80 90 100 110 120
FLENSTFDEF GHSINDYSIS PDGQFILLEY NYVKQWRHSY TASYDIYDLN KRQLITEERI
130 140 150 160 170 180
PNNTQWVTWS PVGHKLAYVW NNDIYVKIEP NLPSYRITWT GKEDIIYNGI TDWVYEEEVF
190 200 210 220 230 240
SAYSALWWSP NGTFLAYAQF NDTEVPLIEY SFYSDESLQY PKTVRVPYPK AGAVNPTVKF
250 260 270 280 290 300
FVVNTDSLSS VTNATSIQIT APASMLIGDH YLCDVTWATQ ERISLQWLRR IQNYSVMDIC
310 320 330 340 350 360
DYDESSGRWN CLVARQHIEM STTGWVGRFR PSEPHFTLDG NSFYKIISNE EGYRHICYFQ
370 380 390 400 410 420
IDKKDCTFIT KGTWEVIGIE ALTSDYLYYI SNEYKGMPGG RNLYKIQLSD YTKVTCLSCE
430 440 450 460 470 480
LNPERCQYYS VSFSKEAKYY QLRCSGPGLP LYTLHSSVND KGLRVLEDNS ALDKMLQNVQ
490 500 510 520 530 540
MPSKKLDFII LNETKFWYQM ILPPHFDKSK KYPLLLDVYA GPCSQKADTV FRLNWATYLA
550 560 570 580 590 600
STENIIVASF DGRGSGYQGD KIMHAINRRL GTFEVEDQIE AARQFSKMGF VDNKRIAIWG
610 620 630 640 650 660
WSYGGYVTSM VLGSGSGVFK CGIAVAPVSR WEYYDSVYTE RYMGLPTPED NLDHYRNSTV
670 680 690 700 710 720
MSRAENFKQV EYLLIHGTAD DNVHFQQSAQ ISKALVDVGV DFQAMWYTDE DHGIASSTAH
730 740 750 760 770 780
QHIYTHMSHF IKQCFSLPDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV
790 800 810 820 830 840
VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK
850 860 870 880 890 900
VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE
910 920 930 940 950 960
SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS
LSPGK
M.W.: Calculated molecular mass (kDa): 111; Estimated by SDS-PAGE under reducing condition (kDa): ~120
Calculated PI: 5.84
Calculated extinction coefficients (M-1 cm-1, at 280nm): 229975
Purity: >92% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation: Supplied at 0.25 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Bioactivity: Binds human CXCL12 and SDF1b proteins by ELISA and cleaves Xaa-proline dipeptides such as the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC)
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
1. J. Biol. Chem. 267:4824-4833(1992)
2. J. Immunol. 149:481-486(1992)
3. Eur. J. Biochem. 267:5608-5613(2000)
4. J. Exp. Med. 177:1135-1143(1993)
5. Science 261:466-469(1993)
6. Cancer Res. 66:4652-4661(2006)
Additional supporting documents, including PDS, COA and MSDS are available upon request.
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.
