Human TPBG/5T4/WAIF1/M6P1/5T4AG Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Description

TPBG (trophoblast glycoprotein), also known as oncofetal antigen 5T4 and WAIF1 (wnt-activated inhibitory factor 1), is a single-pass type I transmembrane glycoprotein of the LRR (leucine-rich repeat) protein family.  TPBG is an oncofetal antigen that is specific to trophoblast cells except for amniotic epithelium. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.  In adults its expression is limited to a few epithelial cell types but highly abundant in many types of  tumor cells. TPBG’s expression is associated with poor clinical outcome in numerous cancers.  TPBG contains 1 LRRNT (LRR N-terminal cap), 7 LRR (LRR1-7), and 1 LRRCT (LRR C-terminal cap) domains in the extracellular region. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.  The C-terminal part of LRRNT and LRR1 are essential for the inhibition of the Wnt signaling pathway.  TPBG may also be involved in cell adhesion and motility.

 

 

Gene Symbol: TPBG; 5T4; M6P1; 5T4AG; WAIF1; 5T4M6P1

 

NCBI Gene ID: 7162

 

Uniprot Entry: Q13641

 

Construct Details: Full length human TPBG/5T4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1263 (bp)

 

Gene Insert Sequence:  

ATGCCTGGGGGGTGCTCCCGGGGCCCCGCCGCCGGGGACGGGCGTCTGCGGCTGGCGCGACTAGCGCTGG

TACTCCTGGGCTGGGTCTCCTCGTCTTCTCCCACCTCCTCGGCATCCTCCTTCTCCTCCTCGGCGCCGTT

CCTGGCTTCCGCCGTGTCCGCCCAGCCCCCGCTGCCGGACCAGTGCCCCGCGCTGTGCGAGTGCTCCGAG

GCAGCGCGCACAGTCAAGTGCGTTAACCGCAATCTGACCGAGGTGCCCACGGACCTGCCCGCCTACGTGC

GCAACCTCTTCCTTACCGGCAACCAGCTGGCCGTGCTCCCTGCCGGCGCCTTCGCCCGCCGGCCGCCGCT

GGCGGAGCTGGCCGCGCTCAACCTCAGCGGCAGCCGCCTGGACGAGGTGCGCGCGGGCGCCTTCGAGCAT

CTGCCCAGCCTGCGCCAGCTCGACCTCAGCCACAACCCACTGGCCGACCTCAGTCCCTTCGCTTTCTCGG

GCAGCAATGCCAGCGTCTCGGCCCCCAGTCCCCTTGTGGAACTGATCCTGAACCACATCGTGCCCCCTGA

AGATGAGCGGCAGAACCGGAGCTTCGAGGGCATGGTGGTGGCGGCCCTGCTGGCGGGCCGTGCACTGCAG

GGGCTCCGCCGCTTGGAGCTGGCCAGCAACCACTTCCTTTACCTGCCGCGGGATGTGCTGGCCCAACTGC

CCAGCCTCAGGCACCTGGACTTAAGTAATAATTCGCTGGTGAGCCTGACCTACGTGTCCTTCCGCAACCT

GACACATCTAGAAAGCCTCCACCTGGAGGACAATGCCCTCAAGGTCCTTCACAATGGCACCCTGGCTGAG

TTGCAAGGTCTACCCCACATTAGGGTTTTCCTGGACAACAATCCCTGGGTCTGCGACTGCCACATGGCAG

ACATGGTGACCTGGCTCAAGGAAACAGAGGTAGTGCAGGGCAAAGACCGGCTCACCTGTGCATATCCGGA

AAAAATGAGGAATCGGGTCCTCTTGGAACTCAACAGTGCTGACCTGGACTGTGACCCGATTCTTCCCCCA

TCCCTGCAAACCTCTTATGTCTTCCTGGGTATTGTTTTAGCCCTGATAGGCGCTATTTTCCTCCTGGTTT

TGTATTTGAACCGCAAGGGGATAAAAAAGTGGATGCATAACATCAGAGATGCCTGCAGGGATCACATGGA

AGGGTATCATTACAGATATGAAATCAATGCGGACCCCAGATTAACGAACCTCAGTTCTAACTCGGATGTC

TGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

1. J. Biol. Chem. 269:9319-9324(1994)

2. Mol. Cancer Ther. 1:1129-1137(2002)

3. Dev. Cell 21:1129-1143(2011)

4. Structure 22:612-620(2014) 

5. Semin Cancer Biol. 29:13-20 (2014).

 

 

Additional supporting documents, including product data sheet (PDS), COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.